Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	70,581	C→T	V65V (GTC→GTT)	araC →	DNA‑binding transcriptional dual regulator AraC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	70,581	0	C	T	100.0%	61.2 / NA	21	V65V (GTC→GTT)	araC	DNA‑binding transcriptional dual regulator AraC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/10);  total (11/10)

CAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGC  >  NC_000913/70339‑70792                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                     cAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAAGCAGGGACGAGAATTTGTTTG‑CCGAcc                                                                                                                                                                                                             <  1:237768/251‑1 (MQ=255)     attgACAATTGGGTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAAGCAGGGACGAGAATTTGTTTG‑CCGACCGGGt                                                                                                                                                                                                         <  1:138462/248‑1 (MQ=255)              tGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGATTAATGCCCATCTGGTGGCGGGTTTAAAGCCGATTTAGGCCAACGGTTATCTCGATTTTTTTATCGACCGCACGCTGGGGATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTGAGGGGGGGGGGAAAAATCAGGGAAGAGAATTTGTTTGGCCGAC‑GGGGGATAttttt                                                                                                                                                                                                >  2:227752/1‑250 (MQ=255)                 tttCTTCTCTGACTGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGGTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGGTATATTCTCAATCTCACCCTTCGCGGTCAGGGGGTGGGGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGGGATAtttttctt                                                                                                                                                                                             >  1:193398/1‑247 (MQ=255)                               ggcgggAGTATGAAAATTATGGCTGAAGCGCAAAATGATCCCCGGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGTTTTAACGCCGATTGAGGCCAACGGTTATCGCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTG‑CGGACCGGGTGATATTTGGCTTTTCCCGCCAGgaga                                                                                                                                                                               <  2:78765/248‑1 (MQ=255)                               ggcgggAGTATGAAAATGATGGCTGAAGCGCAAAAGGATCCCCTGCTGCCGGGATACGCTTTTAATGCCCATCTGGTGGCGGGTTTACCGCCGATTGAGGCCAACGGTTATCTCGTTTTTTTTAGCGACCGACCGCTGGGAATGAAAGGCTATATTCTCAATCTCCCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGCCGAGAATTTGTTTG‑CCTACCGGGTGATATTTTGCTTCTCCGGCCAGgaga                                                                                                                                                                               <  2:109244/248‑1 (MQ=255)                                            aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTAc                                                                                                                                                                  <  1:108553/251‑1 (MQ=255)                                                              aaaaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGcc                                                                                                                                                >  2:65854/1‑250 (MQ=255)                                                                              gcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCAcc                                                                                                                                >  1:169641/1‑251 (MQ=255)                                                                                                   ccATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAAGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATCCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTG‑CCGAACGGGTGATCTTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTAATTTCCTCcgc                                                                                                           >  1:20948/1‑251 (MQ=255)                                                                                                                                      aCGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCa                                                                        <  1:58966/251‑1 (MQ=255)                                                                                                                                        ggTTATCTAGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGCGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAat                                                                      >  1:230202/1‑251 (MQ=255)                                                                                                                                                    ttttttATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAATTCAGGGACGAGAATTTGTTTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGGCGTCAATATTTGCCAATa                                                           <  2:252255/250‑1 (MQ=255)                                                                                                                                                       tttATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGAGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACggg                                                       >  2:207117/1‑251 (MQ=255)                                                                                                                                                          ctCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCTCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAGTACggggtt                                                    >  1:754/2‑248 (MQ=255)                                                                                                                                                                      cTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCgg                                         >  2:210485/1‑250 (MQ=255)                                                                                                                                                                                              aaTCTCACCATTCGCGGTCAGGGGGTGGTAAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGTTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAg                <  1:136515/251‑1 (MQ=255)                                                                                                                                                                                                tctcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGTGATATTTAGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTTGTCCGCGCGCCTACTGGCATGAACGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCg              >  2:212424/1‑251 (MQ=255)                                                                                                                                                                                                       aTTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt       <  1:35304/251‑1 (MQ=255)                                                                                                                                                                                                         tCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt      <  1:65854/250‑1 (MQ=255)                                                                                                                                                                                                             ggTCAGGGGGTGGTGAAAAATCAGGGACGCGAATTTGTTTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATCCGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGc  >  2:264121/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                     CAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGC  >  NC_000913/70339‑70792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑