Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	70,434	C→T	N16N (AAC→AAT)	araC →	DNA‑binding transcriptional dual regulator AraC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	70,434	0	C	T	100.0%	79.5 / NA	26	N16N (AAC→AAT)	araC	DNA‑binding transcriptional dual regulator AraC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/15);  total (11/15)

TCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTTGGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACC  >  NC_000913/70194‑70666                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            tCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGTGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATTCCCATCtg                                                                                                                                                                                                                                    <  1:71763/250‑1 (MQ=255)                              gcgTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGCTTTAGGCCaa                                                                                                                                                                                                       >  1:177091/1‑250 (MQ=255)                                                           tGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATcgaccgac                                                                                                                                                                         <  2:38755/250‑1 (MQ=255)                                                                          ttAATAAGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGTGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATGGACCGACCGCTGGGAAT‑GAAAg                                                                                                                                                         <  1:67063/250‑1 (MQ=255)                                                                           taataaGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAgg                                                                                                                                                        <  1:184454/250‑1 (MQ=255)                                                                               aaGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTTGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCTATTTTTTTATCGACCTACCGCTGGGAAT‑GAAAGGTtat                                                                                                                                                    <  1:214938/250‑1 (MQ=255)                                                                                 gCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCTATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATAtt                                                                                                                                                 <  1:6210/251‑1 (MQ=255)                                                                                                 ggTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCAtt                                                                                                                                  <  1:163510/250‑1 (MQ=255)                                                                                                          gaagaGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGGGTATGAAAAGTATGGGTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGGTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTTTCAATCTCACCATTCGCGGTCAgg                                                                                                                        >  1:132369/1‑251 (MQ=255)                                                                                                           aagaGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATGGACCTACCTCTGGGAAG‑GAAAGGTTATATTCTCAATCTCACCATGCGCTGTCAggg                                                                                                                       <  2:265537/251‑1 (MQ=255)                                                                                                                      aaaGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGGTACTCGTTTAATGCCCATCTGGGGGCGGGGTTTACGCCCATTGAGGCCAAC‑GGTTATCTTGATTTTTTTTTCGCCCGACCGCTGGGAAT‑GAAAGGTTTTATTTTCAATTTCACCATTCGCGGGCCGGGGGGGGTGaaa                                                                                                             >  2:263758/1‑250 (MQ=255)                                                                                                                        aGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAAt                                                                                                          >  1:41571/1‑251 (MQ=255)                                                                                                                            gCAGGGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGGAAAGTATGGCTGAAGCGCAAAATGAGCCCCTGCTGCCCGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTTACGTCGTTTGAGGCCAACCGGTT‑TCTCGATTTTTTTATCGACCGACCGCTGGGACTCGAAAGGT‑GTATTTTCAATATCACCCATAGCGGTCCggggggggggacaaatta                                                                                                        >  2:206835/1‑236 (MQ=255)                                                                                                                                   cGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGGTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGGCCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGGGGGGAAAAATCAGGGACg                                                                                                  >  1:62649/1‑248 (MQ=255)                                                                                                                                    ggCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGAATGAAAAGTATGGCTGAAGCGAAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATTGACCGACCGCTGGGCAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACgaga                                                                                               >  1:162181/1‑250 (MQ=255)                                                                                                                                          gTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGt                                                                                         <  2:210747/250‑1 (MQ=255)                                                                                                                                              gATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGGTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATGCGCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGtttg                                                                                      <  1:14504/249‑4 (MQ=255)                                                                                                                                                  cAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAAGCAGGGACGAGAATTTGTTTG‑CCGAcc                                                                               <  1:237768/251‑1 (MQ=255)                                                                                                                                                      attgACAATTGGGTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAAGCAGGGACGAGAATTTGTTTG‑CCGACCGGGt                                                                           <  1:138462/248‑1 (MQ=255)                                                                                                                                                               tGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGATTAATGCCCATCTGGTGGCGGGTTTAAAGCCGATTTAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGCACGCTGGGGAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTGAGGGGGGGGGGAAAAATCAGGGAAGAGAATTTGTTTGGCCGAC‑GGGGGATAttttt                                                                  >  2:227752/1‑250 (MQ=255)                                                                                                                                                                  tttCTTCTCTGACTGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGGTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGGTATATTCTCAATCTCACCCTTCGCGGTCAGGGGGTGGGGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGGGATAtttttctt                                                               >  1:193398/1‑247 (MQ=255)                                                                                                                                                                                ggcgggAGTATGAAAATTATGGCTGAAGCGCAAAATGATCCCCGGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGTTTTAACGCCGATTGAGGCCAAC‑GGTTATCGCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTG‑CGGACCGGGTGATATTTGGCTTTTCCCGCCAGgaga                                                 <  2:78765/248‑1 (MQ=255)                                                                                                                                                                                ggcgggAGTATGAAAATGATGGCTGAAGCGCAAAAGGATCCCCTGCTGCCGGGATACGCTTTTAATGCCCATCTGGTGGCGGGTTTACCGCCGATTGAGGCCAAC‑GGTTATCTCGTTTTTTTTAGCGACCGACCGCTGGGAAT‑GAAAGGCTATATTCTCAATCTCCCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGCCGAGAATTTGTTTG‑CCTACCGGGTGATATTTTGCTTCTCCGGCCAGgaga                                                 <  2:109244/248‑1 (MQ=255)                                                                                                                                                                                             aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTAc                                    <  1:108553/251‑1 (MQ=255)                                                                                                                                                                                                               aaaaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGcc                  >  2:65854/1‑250 (MQ=255)                                                                                                                                                                                                                               gcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCAcc  >  1:169641/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            TCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTTGGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTG‑CCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACC  >  NC_000913/70194‑70666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑