Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	70,371	T→C	intergenic (‑323/‑16)	araB ← / → araC	ribulokinase/DNA‑binding transcriptional dual regulator AraC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	70,371	0	T	C	100.0%	72.7 / NA	23	intergenic (‑323/‑16)	araB/araC	ribulokinase/DNA‑binding transcriptional dual regulator AraC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/12);  total (11/12)

ATGGCATAGCAAAGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTTGGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTG‑CCGACCGGGTGATATTTTGCTG  >  NC_000913/70147‑70605                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              aTGGCATAGCAAAGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAgc                                                                                                                                                                                                                     >  1:210747/1‑251 (MQ=255)            aaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATc                                                                                                                                                                                                          >  2:35304/1‑251 (MQ=255)                                                tCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGTGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATTCCCATCtg                                                                                                                                                                       <  1:71763/250‑1 (MQ=255)                                                                             gcgTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGCTTTAGGCCaa                                                                                                                                          >  1:177091/1‑250 (MQ=255)                                                                                                          tGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATcgaccgac                                                                                                            <  2:38755/250‑1 (MQ=255)                                                                                                                         ttAATAAGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGTGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATGGACCGACCGCTGGGAAT‑GAAAg                                                                                            <  1:67063/250‑1 (MQ=255)                                                                                                                          taataaGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAgg                                                                                           <  1:184454/250‑1 (MQ=255)                                                                                                                              aaGCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTTGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCTATTTTTTTATCGACCTACCGCTGGGAAT‑GAAAGGTtat                                                                                       <  1:214938/250‑1 (MQ=255)                                                                                                                                gCGGGGTTACCGGTTTGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCTATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATAtt                                                                                    <  1:6210/251‑1 (MQ=255)                                                                                                                                                ggTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCAtt                                                                     <  1:163510/250‑1 (MQ=255)                                                                                                                                                         gaagaGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGGGTATGAAAAGTATGGGTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGGTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTTTCAATCTCACCATTCGCGGTCAgg                                                           >  1:132369/1‑251 (MQ=255)                                                                                                                                                          aagaGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATGGACCTACCTCTGGGAAG‑GAAAGGTTATATTCTCAATCTCACCATGCGCTGTCAggg                                                          <  2:265537/251‑1 (MQ=255)                                                                                                                                                                     aaaGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGGTACTCGTTTAATGCCCATCTGGGGGCGGGGTTTACGCCCATTGAGGCCAAC‑GGTTATCTTGATTTTTTTTTCGCCCGACCGCTGGGAAT‑GAAAGGTTTTATTTTCAATTTCACCATTCGCGGGCCGGGGGGGGTGaaa                                                >  2:263758/1‑250 (MQ=255)                                                                                                                                                                       aGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAAt                                             >  1:41571/1‑251 (MQ=255)                                                                                                                                                                           gCAGGGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGGAAAGTATGGCTGAAGCGCAAAATGAGCCCCTGCTGCCCGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTTACGTCGTTTGAGGCCAACCGGTT‑TCTCGATTTTTTTATCGACCGACCGCTGGGACTCGAAAGGT‑GTATTTTCAATATCACCCATAGCGGTCCggggggggggacaaatta                                           >  2:206835/1‑236 (MQ=255)                                                                                                                                                                                  cGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGGTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGGCCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGGGGGGAAAAATCAGGGACg                                     >  1:62649/1‑248 (MQ=255)                                                                                                                                                                                   ggCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGAATGAAAAGTATGGCTGAAGCGAAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATTGACCGACCGCTGGGCAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACgaga                                  >  1:162181/1‑250 (MQ=255)                                                                                                                                                                                         gTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGt                            <  2:210747/250‑1 (MQ=255)                                                                                                                                                                                             gATGCAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGGTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATGCGCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGtttg                         <  1:14504/249‑4 (MQ=255)                                                                                                                                                                                                 cAATATGGACAATTGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAAGCAGGGACGAGAATTTGTTTG‑CCGAcc                  <  1:237768/251‑1 (MQ=255)                                                                                                                                                                                                     attgACAATTGGGTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAAGCAGGGACGAGAATTTGTTTG‑CCGACCGGGt              <  1:138462/248‑1 (MQ=255)                                                                                                                                                                                                              tGGTTTCTTCTCTGAATGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGATTAATGCCCATCTGGTGGCGGGTTTAAAGCCGATTTAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGCACGCTGGGGAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTGAGGGGGGGGGGAAAAATCAGGGAAGAGAATTTGTTTGGCCGAC‑GGGGGATAttttt     >  2:227752/1‑250 (MQ=255)                                                                                                                                                                                                                 tttCTTCTCTGACTGGCGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGGTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGGTATATTCTCAATCTCACCCTTCGCGGTCAGGGGGTGGGGAAAAATCAGGGACGAGAATTTGTTTG‑CCGACCGGGGGATAtttttctt  >  1:193398/1‑247 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              ATGGCATAGCAAAGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGGTTGGGTTAGCGAGAAGAGCCAGTAAAAGACGCAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAC‑GGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAAT‑GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTG‑CCGACCGGGTGATATTTTGCTG  >  NC_000913/70147‑70605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑