Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,752	T→A	G99G (GGA→GGT)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,752	0	T	A	97.0%	100.0 / NA	33	G99G (GGA→GGT)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (16/16);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

ACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTT‑CAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGC  >  NC_000913/69520‑69967                                                                                                                                                                                                                                          |                                                                                                                                                                                                                        ccgaggCGGCAGATTGCCCCACGGCGCTGTCCGGGCGATTCACAGGCAGGATTTTTGCCCAAACCCATTCGCTGAAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGt                                                                                                                                                                                                       <  1:10732/246‑1 (MQ=255)     tGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCgg                                                                                                                                                                                                   >  2:142599/1‑251 (MQ=255)                       ggCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGt                                                                                                                                                                                 >  1:38755/1‑251 (MQ=255)                            tGTCCTGGCGAGCCAAATGATGGTTTTTTGCCAAGAACCTTACCCTGAAAAAAACTCCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAAc                                                                                                                                                                             <  2:18064/250‑1 (MQ=255)                                    cGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATccc                                                                                                                                                                    >  1:92513/1‑251 (MQ=255)                                            aTGCAGGATTTTTGCCCAGAACCATTCGCTGGAAAAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAg                                                                                                                                                            <  1:65023/251‑1 (MQ=255)                                                      tttGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGAGGGAGTAGTCAACATTGCAAGGCGCGTGGCACAACCGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCCCAATACGAACATCGCGTTCGGGCTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACACCAGCTGCGCGCTg                                                                                                                                                  >  1:125842/1‑251 (MQ=255)                                                         gccaagaacatttCGCTGAAAGAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCTAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGtt                                                                                                                                                <  1:179603/239‑1 (MQ=255)                                                                    ttCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTAAGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGct                                                                                                                                    >  1:136312/1‑251 (MQ=255)                                                                         gggAAAAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGGCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCa                                                                                                                               <  1:10892/250‑1 (MQ=255)                                                                          gaaataaataCCGCCAATTTAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCGCGTTACCGTCGGCATCAATCGGTGCGGGCGTCGGGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTTTTCGACGCTAAGCTCTGCa                                                                                                                               <  1:70613/248‑1 (MQ=255)                                                                          gaaaaaaatcccgccaATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCa                                                                                                                               <  2:84797/237‑1 (MQ=255)                                                                            aataaataACGCCAATATAGCGGGAGCGGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGACTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGTGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGc                                                                                                                            >  2:102407/1‑251 (MQ=255)                                                                                           tatAGCGGGAGCAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GATTCTTTCAACCGCAGGGTGGTCTTTCAACAATACGAACATCGCGTTCGGGTATTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGAGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCCGCGCGCTGTTCGACGCTAAGATCTGCAAGCACGGTTTTCAGTGcg                                                                                                             >  2:237768/3‑250 (MQ=255)                                                                                                           aacattGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCa                                                                                              >  2:234338/5‑250 (MQ=255)                                                                                                            acattGCCCGGCGCGTGGCAAAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGACGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGTTTTTCGGCAAACTCAGGGCGCAGCGCCAACACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAAt                                                                                            >  2:214938/4‑251 (MQ=255)                                                                                                                   ccGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCTTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAATCACGGTTTTCAGTGCCGCTTCCATTGCCTCAATGTAGTCa                                                                                     <  2:20760/251‑1 (MQ=255)                                                                                                                   caggcgcgcagCACAAACGGGTAATATCTTA‑GCTTCTTTCACCGGCAGTGTGGTCTTTCCACAATACGAACATCGCGATCGGCTTTCCGGCAAACTCAGGGCGCAGCTCCAGCACGTTACCGTCGGCATCAATCGGTGCGGCCGTCGAGCCGGTAATGTCAACGCCAATCCCGACCACAGCTGCGAGCTGTTCGACGCTAACCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCATTGTAGTc                                                                                      >  2:6210/11‑250 (MQ=255)                                                                                                                            ggCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGgatg                                                                             <  1:191692/250‑1 (MQ=255)                                                                                                                              cacaAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGatgatg                                                                          <  2:97976/251‑1 (MQ=255)                                                                                                                                caAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCCCGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGAc                                                                        <  1:138188/251‑1 (MQ=255)                                                                                                                                                 tcGCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTAtt                                                           <  1:102096/247‑1 (MQ=255)                                                                                                                                                   gCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCgggg                                                      <  1:183445/251‑1 (MQ=255)                                                                                                                                                   cttcttt‑cAACCGCAGTGTGGTCTTTCCACAATACGACCATCGCGTTCGGGTTCTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGCGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGc                                                     >  1:40099/6‑251 (MQ=255)                                                                                                                                                         t‑cAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATcac                                                <  1:121948/250‑1 (MQ=255)                                                                                                                                                            aaCCGCAGTGTGGTCTTTCTACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACaa                                              >  1:125467/1‑250 (MQ=255)                                                                                                                                                                     gtgGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCtt                                    <  1:210297/251‑1 (MQ=255)                                                                                                                                                                     gtgGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACGCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCt                                     <  2:211181/250‑1 (MQ=255)                                                                                                                                                                          cTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGc                               >  1:78765/1‑251 (MQ=255)                                                                                                                                                                                 cactACGAACATCGCGTTCGGGGTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATCGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACggg                        <  2:136332/248‑1 (MQ=255)                                                                                                                                                                                       gAACCTCGCGTTCGGGATTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGACCTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGTGATAcc                  >  2:71763/1‑251 (MQ=255)                                                                                                                                                                                            tCGCGATCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACCGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGACCTGGGTAATCCGGGAATCACCAACTTGCCCTTTTTGCCAACGGGGCGACCActcc             >  2:67063/1‑250 (MQ=255)                                                                                                                                                                                                       ttttCGGCCAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCACTCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGc  >  2:157483/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                        ACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTT‑CAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGC  >  NC_000913/69520‑69967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑