Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,629	G→A	N140N (AAC→AAT)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,629	0	G	A	100.0%	82.0 / NA	25	N140N (AAC→AAT)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/14);  total (11/14)

CGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTT‑CAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCC  >  NC_000913/69403‑69860                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                         cgccCCAGCTTTCGTGCCACAGAGTTTTATGCCCGGCGCTGCAACGTCCGCGCCGAATATCCTGGGGGCGGGTGGTACCGGAAAGCAGAGCTGGCCCCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGAAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACgg                                                                                                                                                                                                                  <  1:219271/251‑1 (MQ=255)                             gcgCCCGGCGCGGCAACGCCCGCAACGAATACCCGGCGGGGGGGGGTTACGGGAAAGCAGAGCGGCCCCCCCGTCGCCCACCGCAATCCACGATGCGGCAGATGCCGCCAGGGCGGTGTCCTGGCGGGTCACAGGCGGTATTTTTGCCCAGACCCATTCGCGGGAATAAATCCCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAg                                                                                                                                                                                     <  2:257950/249‑1 (MQ=255)                                      cgtgaaaCGGCCGCGACGAATATCCTGCGGGCGGGTGGCACCGGAAAGCAGAGCTGCCACCCAGTCGAACAGCTCAACCCCCGAGGCGGCAGATTGCGCCACGGCGCTGTCCTGGGGAGTCACATGCAGGATTTTTGCCCAGACCCATCCGCGGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGTTCtt                                                                                                                                                                            <  2:65206/246‑1 (MQ=255)                                          cAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTcc                                                                                                                                                                         >  1:211181/1‑250 (MQ=255)                                            aCGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCcac                                                                                                                                                                       >  2:212759/1‑250 (MQ=255)                                              gTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAAt                                                                                                                                                                    >  2:65023/1‑251 (MQ=255)                                                   ggacgaATATCCTGCGGGCGGTGGGTACCGGAAAGCAGAGCGGGCACCAATTCGCACAGACAAATCAACGTGGCGGAAGATTGCCCCACGGCGCTGTCCTGGCGGGTAACATGAAGGATTTTTGCCCAGAACCATTCGCTGGAATAAACACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGaa                                                                                                                                                               <  1:233659/250‑1 (MQ=255)                                                   cgacgaATAGCCGGGGGGGGGGTGGGACCGAAAAGCAGAGCTGGCCCCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGaa                                                                                                                                                               <  2:95784/251‑1 (MQ=255)                                                             ccTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTc                                                                                                                                                     >  1:84797/1‑251 (MQ=255)                                                                               cGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCCCGAGGCGGCAGATTGCGCCACGGCGCTGCCCTGGGGATTCACAGGCAGGATTTTTGCCCAGACCCATGCGCTGAAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTGCGGCAAACTcc                                                                                                                                   <  1:202000/251‑1 (MQ=255)                                                                                    aGCAGAGCTGGCCCCCAGTCGCACAGCTCAATCCACGATGCGGCAGATGCCGCCCCGGCGCTTTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGACCCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGgcg                                                                                                                              <  1:153864/251‑1 (MQ=255)                                                                                                                      ccgaggCGGCAGATTGCCCCACGGCGCTGTCCGGGCGATTCACAGGCAGGATTTTTGCCCAAACCCATTCGCTGAAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGt                                                                                            <  1:10732/246‑1 (MQ=255)                                                                                                                          tGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCgg                                                                                        >  2:142599/1‑251 (MQ=255)                                                                                                                                            ggCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGt                                                                      >  1:38755/1‑251 (MQ=255)                                                                                                                                                 tGTCCTGGCGAGCCAAATGATGGTTTTTTGCCAAGAACCTTACCCTGAAAAAAACTCCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAAc                                                                  <  2:18064/250‑1 (MQ=255)                                                                                                                                                         cGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATccc                                                         >  1:92513/1‑251 (MQ=255)                                                                                                                                                                 aTGCAGGATTTTTGCCCAGAACCATTCGCTGGAAAAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAg                                                 <  1:65023/251‑1 (MQ=255)                                                                                                                                                                           tttGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGAGGGAGTAGTCAACATTGCAAGGCGCGTGGCACAACCGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCCCAATACGAACATCGCGTTCGGGCTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACACCAGCTGCGCGCTg                                       >  1:125842/1‑251 (MQ=255)                                                                                                                                                                              gccaagaacatttCGCTGAAAGAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCTAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGtt                                     <  1:179603/239‑1 (MQ=255)                                                                                                                                                                                         ttCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTAAGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGct                         >  1:136312/1‑251 (MQ=255)                                                                                                                                                                                              gggAAAAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGGCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCa                    <  1:10892/250‑1 (MQ=255)                                                                                                                                                                                               gaaataaataCCGCCAATTTAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCGCGTTACCGTCGGCATCAATCGGTGCGGGCGTCGGGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTTTTCGACGCTAAGCTCTGCa                    <  1:70613/248‑1 (MQ=255)                                                                                                                                                                                               gaaaaaaatcccgccaATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCa                    <  2:84797/237‑1 (MQ=255)                                                                                                                                                                                                 aataaataACGCCAATATAGCGGGAGCGGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGACTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGTGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGc                 >  2:102407/1‑251 (MQ=255)                                                                                                                                                                                                                tatAGCGGGAGCAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GATTCTTTCAACCGCAGGGTGGTCTTTCAACAATACGAACATCGCGTTCGGGTATTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGAGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCCGCGCGCTGTTCGACGCTAAGATCTGCAAGCACGGTTTTCAGTGcg  >  2:237768/3‑250 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                         CGCCCCAGCTTTCGTGCCACAGAGATTTATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTT‑CAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCC  >  NC_000913/69403‑69860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑