Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,421,8350AG25.0% 75.3 / 15.7 40Y43C (TAC→TGC) ulaCL‑ascorbate specific PTS enzyme IIA component
Reads supporting (aligned to +/- strand):  ref base A (17/13);  new base G (7/3);  total (24/16)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATACGTGGTTGGCGTGCGCAACATGCTCTCTCCTGCCGACTTTGGCCCGAAACTGCTGGAAGTGATCAAAGAGCATTTCCCGCAGGATGTGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGCCAATACTCACCAGGAAGTGGGCATCATGC  >  NC_000913/4421602‑4422065
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                      
aTACGGGGTTGGCGTGCGCAACATGCTCTCTCCTGCCGACTTTGGCCCGAAACTGCTGGAAGTGATCAAAGAGCATTTCCCGCAGGATGTGAAGTAAGGACGCGCCAGGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTgg                                                                                                                                                                                                                       <  1:241217/251‑1 (MQ=255)
      ggTTGGCGTGCGCAACATGCTCTCTCCTGCCGACTTTGGCCCGAAACTGCTGGAAGTGATCAAAGAGCATTTCCCGCAGGATGTGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCg                                                                                                                                                                                                                 <  2:88770/251‑1 (MQ=255)
                cgcAACATGCTCTCTCCTGCCGACTTTGGCCCGAAACTGCTGGAAGTGATCAAAGAGCATTTCCCGCAGGATGTGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGtt                                                                                                                                                                                                       <  1:8478/251‑1 (MQ=255)
                      aTGCTCTCTCCTGCCGACTTTGGCCCGAAACTGCTGGAAGTGATCAAAGAGCATTTCCCGCAGGATGTGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTcc                                                                                                                                                                                                 >  1:141641/1‑251 (MQ=255)
                      aTGCTCTCTCCTGCCGACTTTGGCCCGAAACTGCTGGAAGTGATCAAAGAGCATTTCCCGCAGGATGCGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTcc                                                                                                                                                                                                 >  2:43878/1‑251 (MQ=255)
                        gCTCTCTCCTGCCGACTTTGGCCCGAAACTGCTGGAAGTGATCAAAGAGCATTTCCCGCAGGATGTGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCtt                                                                                                                                                                                               <  1:49772/251‑1 (MQ=255)
                                  gCCGACTTTGGCCCGAAACTGCTGGAAGTGATCAAAGAGCATTTCCCGCAGGATGTGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGGGAGGCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTAt                                                                                                                                                                                     <  1:24357/251‑1 (MQ=255)
                                      aCTTTGGCCCGAAACTGCTGGAAGTGATCAAAGAGCATTTCCCGCAGGATGTGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCt                                                                                                                                                                                 >  1:189180/1‑251 (MQ=255)
                                       cTTTGGCCCGAAACTGCTGGAAGTGATCAAAGAGCATTTCCCGCAGGATGTGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTc                                                                                                                                                                                >  1:191659/1‑251 (MQ=255)
                                               cGAAACTGCTGGAAGTGATCAAAGAGCATTTCCCGCAGGATGTGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTGCTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTg                                                                                                                                                                        <  1:257795/251‑1 (MQ=255)
                                                      gctgGAAGTGATCAAAGAGCATTTCCCGCAGGATGTGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGc                                                                                                                                                                 >  2:103301/1‑251 (MQ=255)
                                                       ctgGAAGTGATCAAAGAGCATTTCCCGCAGGATGTGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGcc                                                                                                                                                                >  1:239389/1‑251 (MQ=255)
                                                                 tCAAAGAGCATTTCCCGCAGGATGTGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGCGCCGCGTTGCTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCCATGCCGCACGGGCGt                                                                                                                                                      >  1:162993/1‑251 (MQ=255)
                                                                                cGCAGGATGTGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGtt                                                                                                                                       <  1:190183/251‑1 (MQ=255)
                                                                                   aGGATGTGAAGTAAGGACGCACCATGAAGTTACGTGAGTCGCTGGAGGAAAATAAATCCAGCCGCCTGCAGGCTGAAGAAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTTCCAGGCGATTCTGTATGGCGTTGAACAGTTCGGTCCTTATTTCGTTAGCGCTCCGGGCCTGGCAATGCCGCACGTGCGTCCGGAAGAGGGCTTTAAg                                                                                                                                    <  2:172350/251‑1 (MQ=255)
                                                                                                aGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTtct                                                                                                                       <  1:3283/251‑1 (MQ=255)
                                                                                                     gcgcCATGAAATTACGTGCTTCGCTGGCGGAAAATCAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGCACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTg                                                                                                                  >  2:164391/1‑251 (MQ=255)
                                                                                                      cgcCATGAAATTACGTGATACGCAGGCGGAAAATAACTCCATCCGAATGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTAATACCAGGCGATTCTGGATGGCGGTGAACAGGTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGACGCACGGGCGTCCGGAAGGGGGCGTTAAGAAAACCGGATTCTCTCTgg                                                                                                                 >  1:54780/1‑251 (MQ=255)
                                                                                                              aaTTACGTGATACGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCCGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTg                                                                                                         >  1:270869/1‑251 (MQ=255)
                                                                                                                ttACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGACAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGa                                                                                                        >  2:39356/1‑250 (MQ=255)
                                                                                                                 tacgagcttCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTACTTATTTCGTTATCGCTCCGGGCCTGGCAATGACGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGaa                                                                                                       >  2:234658/8‑250 (MQ=255)
                                                                                                                           gctgccgcaaaaTAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGCAGCGGTGGAAATCGGCGTTGATCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTGATACCAGGCGATTCTGCTTGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCTTGGCAATGCCGCACGTGCGTCCTGAAGTGGTTGTTGAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTgg                                                                                            <  1:35259/243‑1 (MQ=255)
                                                                                                                            cTGGCGGAAACTAAATCAATCAGCCTGCAGGCTGAAGCAGAGACATGGCCGGAAGCCGTGACAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTGCTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTgg                                                                                            >  2:120869/1‑250 (MQ=255)
                                                                                                                            cTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGCAGCGGAGAAAATCGGAGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTCCCAGGCGCTTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGCTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTgg                                                                                            >  1:254539/1‑250 (MQ=255)
                                                                                                                                 ggAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCa                                                                                      <  1:155962/251‑1 (MQ=255)
                                                                                                                                             catccGCATGCAGGATGAAGCAGAGATCTGGCAGAACGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGAc                                                                           >  2:167476/1‑250 (MQ=255)
                                                                                                                                                 cGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTGCTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGa                                                                      <  2:82472/251‑1 (MQ=255)
                                                                                                                                                 cGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACg                                                                       >  1:72992/1‑250 (MQ=255)
                                                                                                                                                    cTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTGCTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATcc                                                                   >  2:217234/1‑251 (MQ=255)
                                                                                                                                                       cgtgatGAAGCAGAGACATGGCAGGAAGCGGTGAAAAACGGCGTTGACCTGCTGGTGGCGGCAGATGTTGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCGGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCGCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGt                                                                <  2:4011/246‑1 (MQ=255)
                                                                                                                                                       cAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGt                                                                >  1:260334/1‑251 (MQ=255)
                                                                                                                                                             gAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTGCTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTACGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGatat                                                          >  1:16630/1‑251 (MQ=255)
                                                                                                                                                                  agagACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCCGATGTGGAAGAGCCGCGTTGCTACCAGGCGATTCTGGATGGCGTTGCACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACCGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATctcctc                                                      >  2:165418/1‑250 (MQ=255)
                                                                                                                                                                      aCATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCAc                                                 >  2:3127/1‑251 (MQ=255)
                                                                                                                                                                               gAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTGCTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATggcggc                                        >  2:208395/1‑251 (MQ=255)
                                                                                                                                                                                        aaaaTCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGCTGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGTAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGc                               >  2:41826/1‑251 (MQ=255)
                                                                                                                                                                                             cGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGCCAAt                           <  1:107484/250‑1 (MQ=255)
                                                                                                                                                                                              ggCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGCCAATAc                         <  2:62202/251‑1 (MQ=255)
                                                                                                                                                                                                                   gcAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGCCAATACTCACCAGGAAGTGGGcatcat    <  1:43878/251‑1 (MQ=255)
                                                                                                                                                                                                                     aGATGTGGTAGAGCCGCGTTGCTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGCCAATACTCACCAGGAAGTGGGCATCATGc  >  2:120556/1‑251 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                      
ATACGTGGTTGGCGTGCGCAACATGCTCTCTCCTGCCGACTTTGGCCCGAAACTGCTGGAAGTGATCAAAGAGCATTTCCCGCAGGATGTGAAGTAAGGACGCGCCATGAAATTACGTGATTCGCTGGCGGAAAATAAATCCATCCGCCTGCAGGCTGAAGCAGAGACATGGCAGGAAGCGGTGAAAATCGGCGTTGACCTGCTGGTGGCGGCAGATGTGGTAGAGCCGCGTTACTACCAGGCGATTCTGGATGGCGTTGAACAGTTCGGTCCTTATTTCGTTATCGCTCCGGGCCTGGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGCCAATACTCACCAGGAAGTGGGCATCATGC  >  NC_000913/4421602‑4422065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑