Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,980,7920CG25.8% 57.4 / 21.1 31G464A (GGC→GCC) araEarabinose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (8/15);  new base G (4/4);  total (12/19)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGCCGCTGTTTAATGCCTTCATTTATCAAACGATAGTAAGGAATGGTGGATTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTC  >  NC_000913/2980547‑2981027
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           
aGCCGCTGTTTAATGCCTTCATTTATCAAACGATAGTAAGGAATGGTGGATTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGcc                                                                                                                                                                                                                                        >  2:36735/1‑251 (MQ=255)
              gCCTTCATTTATCAAACGATAGTAAGGAATGGTGGATTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGGCTGCCATCAGTTTGCGTTc                                                                                                                                                                                                                          <  1:125016/251‑1 (MQ=255)
              gCCTTCATTTATCAAACGATAGTAAGGAATGGTGGATTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTc                                                                                                                                                                                                                          <  2:19643/251‑1 (MQ=255)
                  tCATTTATCAAACGATAGTAAGGAATGGTGGATTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGGCTGCCATCAGTTTGCGTTCGat                                                                                                                                                                                                                       >  2:196474/1‑250 (MQ=255)
                                                        gCTCATTCCTCCCAGCAAAACAATTGTTTTCATTCCGCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTGCCAGCGTGACATTTTTGGTTTCCGGAATTAGCCa                                                                                                                                                                                <  2:208264/251‑1 (MQ=255)
                                                                ctccCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTa                                                                                                                                                                         >  1:82574/1‑250 (MQ=255)
                                                                  cccAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGACTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAAt                                                                                                                                                                       <  2:103100/250‑1 (MQ=255)
                                                                        aaaCCAATTGTTTGCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCGCAACGGCCAGGTGCCCAATCTCTATTAACGAAAAAAGGGCCGTATGTACAGCACATCCTGCCTGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCcaca                                                                                                                                                                <  1:267732/251‑1 (MQ=255)
                                                                         aaCCAATTGTTGTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGTGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGTGCCAGAAAGTAATGCCcacta                                                                                                                                                               <  2:38377/251‑3 (MQ=255)
                                                                                tGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTGTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCaa                                                                                                                                                         <  1:29257/250‑1 (MQ=255)
                                                                                          ccTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGCCAACAGCATCAACGGCCAAGTGCCCAATATCTATTAACGAAAAAAGGGACGGATGTCCCGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAgcgc                                                                                                                                               >  1:266460/1‑250 (MQ=255)
                                                                                                        tgtgtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAACAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGGCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGa                                                                                                                                 >  2:132762/1‑250 (MQ=255)
                                                                                                                cGCAACAAGGCTGGATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCTACTTCTCGGCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCgg                                                                                                                        >  2:186483/1‑251 (MQ=255)
                                                                                                                     caatgCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGTAAGGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAgcg                                                                                                                   <  1:23397/247‑1 (MQ=255)
                                                                                                                       aGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGcc                                                                                                                 <  1:10335/251‑1 (MQ=255)
                                                                                                                              aTAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTa                                                                                                          <  1:247210/251‑1 (MQ=255)
                                                                                                                                    aaCGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTAGCAAGc                                                                                                    <  1:229676/251‑1 (MQ=255)
                                                                                                                                               cGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGGCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGaa                                                                                         <  1:108367/251‑1 (MQ=255)
                                                                                                                                                    cAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGGCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCATGAAGGt                                                                                      <  2:42262/249‑1 (MQ=255)
                                                                                                                                                             aGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATa                                                                            >  1:191425/1‑250 (MQ=255)
                                                                                                                                                                       ctctATTAACGAAAAACGGGCCGGATGTACCGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCACGCAGTGTCAGGAAGGTCGCGCCGATAATCCTATTCg                                                                  >  2:22549/1‑250 (MQ=255)
                                                                                                                                                                                 gAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCCGCGTGACCTTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCCATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTc                                                       >  2:106749/1‑251 (MQ=255)
                                                                                                                                                                                               aTGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAAc                                          >  1:53614/1‑250 (MQ=255)
                                                                                                                                                                                                      gCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGTAAGGTCGCGCCGATAAGCAGATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATa                                  <  1:253970/251‑1 (MQ=255)
                                                                                                                                                                                                                cccGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATcgc                        <  2:82574/251‑1 (MQ=255)
                                                                                                                                                                                                                  cGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCgg                      <  2:2308/251‑1 (MQ=255)
                                                                                                                                                                                                                               gCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCt         <  1:268574/251‑1 (MQ=255)
                                                                                                                                                                                                                                ccGATATTTCTCAACTTCTCGGCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTGCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTg        <  1:130345/251‑1 (MQ=255)
                                                                                                                                                                                                                                     atTTCTCAACTTCTCGGCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGCGACAGAAAGTAATGCACACAAACGAAATGTTCAGCGCCGTGTAGAGCCAGAACGTACCGTCAGCGCCAATGCTATCACGCAGTGTCAGGAAGGTCGCGCCGATAATCCTATTCGACACCCAGTTCGTGGAGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAAttt   >  1:35010/1‑251 (MQ=255)
                                                                                                                                                                                                                                     atTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAAttt   <  1:149670/251‑1 (MQ=255)
                                                                                                                                                                                                                                        tctcAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTc  >  1:75551/1‑249 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           
AGCCGCTGTTTAATGCCTTCATTTATCAAACGATAGTAAGGAATGGTGGATTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTC  >  NC_000913/2980547‑2981027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑