Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,972,6060TC36.7% 20.6 / 26.1 31V337A (GTG→GCG) tasputative NADP(H)‑dependent aldo‑keto reductase Tas
Reads supporting (aligned to +/- strand):  ref base T (8/11);  new base C (4/7);  total (12/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCC  >  NC_000913/2972371‑2972843
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             
tttAGTCGGTTCACCTGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATAc                                                                                                                                                                                                                                >  1:74331/1‑251 (MQ=255)
 ttAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTtata                                                                                                                                                                                                                                 <  1:81624/249‑1 (MQ=255)
       ggTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCg                                                                                                                                                                                                                         <  1:39051/251‑1 (MQ=255)
       ggTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCg                                                                                                                                                                                                                         >  2:109297/1‑251 (MQ=255)
                           gAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCg                                                                                                                                                                                                     <  2:269318/251‑1 (MQ=255)
                              cAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGtt                                                                                                                                                                                                  <  2:8343/251‑1 (MQ=255)
                                 aCGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAACACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGCGCATCCGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTaa                                                                                                                                                                                                >  2:126076/1‑250 (MQ=255)
                                      aaaaGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCGGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCTGGGCAGCAGGTTTAGACGTATCCGGCACCATAAGATTAACGCCGGTTAAAACCgg                                                                                                                                                                                          <  1:133047/251‑1 (MQ=255)
                                           ccGTCGCGGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTa                                                                                                                                                                                     <  1:231849/251‑1 (MQ=255)
                                               cgcgGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGCGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAata                                                                                                                                                                                 <  2:73764/251‑1 (MQ=255)
                                                  ggcgtttgtagttatCGCCAGAAGTCCTGGCATGCACCCTGCTCAGATGGCGCCCGCGTCTGTCCGCCGGCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCAAGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTCTTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATCAGATTAACGCCGGTTAAAACCGGCGTTAAatcttc                                                                                                                                                                              >  2:247792/13‑247 (MQ=255)
                                                    cgTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGCTGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCCTCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGCGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAAt                                                                                                                                                                            >  1:135778/1‑251 (MQ=255)
                                                       aTGTTGATATCGGCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTATGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTGTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGAc                                                                                                                                                                         <  2:253167/251‑1 (MQ=255)
                                                       aTGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGAc                                                                                                                                                                         >  2:255482/1‑251 (MQ=255)
                                                             atatCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTTAAATTGAAGCTGTGCATCATGTTTATACTTATCCGGCTCCATAAGATTAACTCCGGTTAAAACCGGCGTGAAATATTAATGACTGCGCt                                                                                                                                                                   <  2:179168/251‑1 (MQ=255)
                                                                           cATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCCTCAACCGGTTGTTGCCAGCACTCTGCTGGGCGCAGCCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAAGTGAAGCGGCGCAACATGTTTGTACTTATCCGGCACCATAAGCTTAACGCCGGTTAAAACCGGCGTGAAATATTAAGGACTGTGCTGCCAGATCCACAgc                                                                                                                                                     <  2:178482/251‑1 (MQ=255)
                                                                             tGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGCGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAgcgc                                                                                                                                                   >  1:240192/1‑251 (MQ=255)
                                                                              ggCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGcc                                                                                                                                                  <  1:4014/251‑1 (MQ=255)
                                                                               gCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCg                                                                                                                                                 <  2:1598/251‑1 (MQ=255)
                                                                                            tCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAgcgc                                                                                                                                    >  2:97327/1‑251 (MQ=255)
                                                                                                                         aaCCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGCGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTGAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGGTATTGCCAGCGCAAACAGCGCTCCGAATCCAATGCCAATgg                                                                                                       <  2:135705/251‑1 (MQ=255)
                                                                                                                               ttgttgCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGCGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATGAATGACGGCGCTGCCAGATCCACAGCGCCGTTATGGCCAGTGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACg                                                                                                  <  1:253530/250‑1 (MQ=255)
                                                                                                                                              tgctgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATc                                                                                   >  2:206481/1‑250 (MQ=255)
                                                                                                                                               gctgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGCGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCAt                                                                                 <  2:218962/251‑1 (MQ=255)
                                                                                                                                                  gggCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGCGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTAc                                                                              <  2:240192/251‑1 (MQ=255)
                                                                                                                                                                  gATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGATGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCACACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTACATg                                                              >  2:107223/1‑251 (MQ=255)
                                                                                                                                                                   aTCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAACCAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTCCCGCCAGCGAGTAAATGc                                                             <  2:28696/251‑1 (MQ=255)
                                                                                                                                                                    tCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGCGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGAAGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACAGAAGCCAATGCCAATCGGCACGACAGGGATGCCTATCATTACCGCCAGCGAGTAAATGcc                                                            >  1:184046/1‑251 (MQ=255)
                                                                                                                                                                                  aCATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAacaaca                                              <  1:255482/251‑1 (MQ=255)
                                                                                                                                                                                    aTCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAAGTGAAGCGGCGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATg                                            <  1:115834/251‑1 (MQ=255)
                                                                                                                                                                                                                              gAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTcccc  >  1:114426/1‑251 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             
TTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCC  >  NC_000913/2972371‑2972843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑