Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913266,3580TG40.9% 14.0 / 10.8 23T66P (ACC→CCC) yafYinner membrane lipoprotein YafY
Reads supporting (aligned to +/- strand):  ref base T (1/12);  new base G (9/0);  total (11/12)
Fisher's exact test for biased strand distribution p-value = 2.01e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGT‑AAAA‑CCTGCAA‑GGTGGGACCGGATAAGTAG‑TCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGTTTCATTATTTTTTTCCTGAAATCAGACGAACCACTTTGGCAAAGACATAAATGCCCAC  >  NC_000913/266115‑266606
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         
ccTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGT‑AAAA‑CCTGCAA‑GGTGGGACCg                                                                                                                                                                                                                                                    <  1:116342/251‑1 (MQ=255)
                 gAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGT‑CAAA‑CCCGCCA‑GGGGGGGCCGGGTaaacaggcaaccct                                                                                                                                                                                                                                   >  1:93329/1‑239 (MQ=255)
                   gggTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTATTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGT‑AAAA‑CCTGCAA‑GGTGGGACCGGATAAGTAG‑TCAACCtgtg                                                                                                                                                                                                                                <  1:156511/251‑1 (MQ=255)
                    ggTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGC‑AAAA‑ACTGCAA‑AGGGGGGCCGGGGAAGGAG‑TCCACCtgtgt                                                                                                                                                                                                                               >  1:140176/1‑250 (MQ=255)
                              gCTGCCCCATCCGTTTGCCGTCATTGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGT‑AAAA‑CCTGCAA‑GGTGGGACCGGATAAGTAG‑TCAACCTGTGACCATTCTTCg                                                                                                                                                                                                                     <  2:1475/251‑1 (MQ=255)
                                                        gCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGTCGTCAAAA‑CC‑GCAAAGGGGGGCCCGG‑TAAGGGGGCCAACC‑GTGACCATTCTTCCCTGGCATCATCCTGGTGGCTGAcccc                                                                                                                                                                                           >  1:209355/1‑249 (MQ=255)
                                                                               ggTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGT‑AAAA‑CCTGCAA‑GGTGGGACCGGATAAGTAG‑TCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGa                                                                                                                                                                    <  1:113645/251‑1 (MQ=255)
                                                                                gTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAGGT‑AAAA‑ACTGCAA‑GGGGGGACCGGAGAAGAAG‑TCAACCTGTGGCCATTCTTTGCTGACATCATCCTTGGGGGTGACCACATCACTGAAACGCGgggggggggg                                                                                                                                                                   >  1:228713/1‑247 (MQ=255)
                                                                                       ggTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGT‑AAAA‑ACTGCAA‑GGGGGGGCCGGAGAAGTAG‑TCAACCTGTGACCATTTTTTGCTGACATAATCCTGGTGGCTTGCCACATCACTGAAAAGCGGTGGGGGGAGGGCCttt                                                                                                                                                            >  1:142735/1‑250 (MQ=255)
                                                                                         tCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAGGT‑AAAAACCCGCAA‑G‑AGGGACCCGCGAAGAAG‑TCAAACTGGTAGCAATCTTTGCTGACATCATCCCGGTGGGGGACCACCTCACTTAAACACGGGGGGGGGGGGGCCTTaa                                                                                                                                                           >  1:78598/1‑250 (MQ=255)
                                                                                                                    aTGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGT‑AAAA‑ACCGCCA‑GGGGGGACCGGGGAAGTAG‑TCAACCTGTGACCATTCTTCGCTGTCATCCTCCTGGCGGGGGCCCACACCCCTGAAACCCCGTGGGGGGGGGGCCTTTAATTTattgttgtccttttggtcatacct                                                                                                                               >  2:194828/1‑228 (MQ=255)
                                                                                                                                              catcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGGCGT‑AAAA‑ACCGCCA‑GGGGGGGCCGGGTAAGTAG‑TCAACCCGTGGCCATTCTTCGCTGGCATCCTCCTGGTGGGTGCCCACATAACTACAACACGGGGGGGGGGGGGCCTTTAATTTACTTGTGGCCCTTAGGGCATACCTTCTCTCTTCGCATGCGATGGGaaaa                                                                                                      >  2:65004/1‑249 (MQ=255)
                                                                                                                                                      gCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGT‑AAAA‑CCTGCAA‑GGTGGGACCGGATAAGTAG‑TCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAgtggtg                                                                                             <  2:47422/251‑1 (MQ=255)
                                                                                                                                                             ggTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGT‑AAAA‑CCTGCAA‑GGTGGGACCGGATAAGTAG‑TCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCATGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCa                                                                                      <  1:26253/251‑1 (MQ=255)
                                                                                                                                                                                   aGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGT‑AAAA‑CCTGCAA‑GGTGGGACCGGATAAGTAG‑TCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGtttt                                                                <  1:239155/251‑1 (MQ=255)
                                                                                                                                                                                   aGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAAGT‑AAAA‑CCCGCAA‑GGGGGGACCGGGTAAGTAG‑TTAACCTGTGACCATTCTTTGCTGGCATCATCCCGGTGGGGGACCACATCACTCAAACGCGGGGGGGGGGGGGCCTTAAATTTACTTATGGCCTTCAGGGCCTCACTTCTTTCATCGCCTGCGAAGAGAAAAAAAGGGGGGGCAACCCGCGCCAGCAGAGGCCGTGgttt                                                                >  1:180733/1‑251 (MQ=255)
                                                                                                                                                                                             aaCTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGT‑AAAA‑CCTGCAA‑GGTGGGACCGGATAAGTAG‑TCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGTTTCatt                                                      <  1:215059/251‑1 (MQ=255)
                                                                                                                                                                                             aaCTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGT‑AAAA‑CCTGCAA‑GGGGGGGCCGGATAAGGAG‑TCAACCTGGGACCATTCTTCGCTGACATCATCCTTGTGGGTGGCCACATCACTTAAACGGGGGGGGGGGAGGGCCTTTAATTTACTGATGGCCTTCAGGTCATCCCTTCTTTCATCGCATGCGATGGGAAAAAAAGGGGTGGCAACCAGCGCCAGCAGAGGGAGTGTTTTACGttttttt                                                      >  1:61898/1‑247 (MQ=255)
                                                                                                                                                                                              aCTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGT‑AAAA‑CCTGCAA‑GGTGGGACCGGATAAGTAG‑TCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGTTTCatta                                                     <  2:180733/251‑1 (MQ=255)
                                                                                                                                                                                                             gCCATCGGGCGACTGGCGGGTACGT‑AAAA‑CCTGCAA‑GGTGGGACCGGATAAGTAG‑TCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGTTTCATTATTTTTTTCCTGAAAt                                      <  1:202344/251‑1 (MQ=255)
                                                                                                                                                                                                                                               gCAA‑GGTGGGACCGGATAAGTAG‑TCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGTTTCATTATTTTTTTCCTGAAATCAGACGAACCACTTTGGCAAAGACATAAATGc      >  1:237718/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 aa‑GGTGGGACCGGATAAGTAG‑TCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGTTTCATTATTTTTTTCCTGAAATCAGACGAACCACTTTGGCAAAGACATAAATGccc    <  2:78598/251‑1 (MQ=255)
                                                                                                                                                                                                                                                    ggTGGGACCGGATAAGGAG‑TCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTTGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCATCGCCAGCAGAGGCAGTGTTTTACGTTTCATTATTTTTTTCCTGAAATCAGACGAACCACTTTGGCAAAGACATAAATGCCCAc  <  1:264151/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         
CCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGT‑AAAA‑CCTGCAA‑GGTGGGACCGGATAAGTAG‑TCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGTTTCATTATTTTTTTCCTGAAATCAGACGAACCACTTTGGCAAAGACATAAATGCCCAC  >  NC_000913/266115‑266606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑