Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,353,837	IS2 (+) +5 bp	coding (877‑881/1518 nt)	lysU ←	lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3186096 =	NA (NA)	21 (0.810)	18/490	0.5	83.7%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	= 4353841	4 (0.160)					coding (877/1518 nt)	lysU	lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase
*	?	NC_000913	4353837 =	5 (0.200)	21 (0.810)	21/492	0.3	80.5%	coding (881/1518 nt)	lysU	lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

TCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGC  >  NC_000913/3185847‑3186340                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      tCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACAGCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAAATAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGttatt                                                                                                                                                                                                                                                       <  2:88473/250‑1 (MQ=255)                gCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACTGGCCCCCTGaa                                                                                                                                                                                                                                      <  2:168620/251‑1 (MQ=255)                   aTCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACTGGCCCCCTGAAt                                                                                                                                                                                                                                     <  2:88948/249‑1 (MQ=255)                                       gCTCGTATGGCATCCCCACGCAAAGATGCCCCCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGCGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATATAAACTTAACATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACTGTCCTCCTGAATCTCCATACAACCAATATCACtt                                                                                                                                                                                                               >  2:91823/1‑251 (MQ=255)                                        atCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATAATTAACGGATTAATGATAAGTGGAACAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCAGTTATTA‑GACTGGCCACCTGAATCTCCAGCCAACCAATATccttt                                                                                                                                                                                                               >  2:71004/2‑246 (MQ=255)                                                 aaTCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTg                                                                                                                                                                                                      >  1:136449/1‑250 (MQ=255)                                                       cAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACGGGCCTCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCt                                                                                                                                                                                               <  1:178984/251‑1 (MQ=255)                                                           cAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACTGGCCCCCGGAATCTCCAGACAACCAATATCTCTTAAATAAGTGATAGTCTTAAt                                                                                                                                                                                           <  1:235799/251‑1 (MQ=255)                                                               gATGCCACCCGGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACt                                                                                                                                                                                        >  1:230397/1‑250 (MQ=255)                                                                              aaCGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTc                                                                                                                                                                        >  2:242751/1‑251 (MQ=255)                                                                                 gTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATGA‑GACTGGCCCCCTGAATCTCCAGACAACCAATATCACGTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCAtt                                                                                                                                                                     <  1:179074/251‑1 (MQ=255)                                                                                                 aCTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAtgattg                                                                                                                                                     <  1:3801/251‑1 (MQ=255)                                                                                                               tACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTAGTGTTCATTATCCGTTATTA‑GACTGGACCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTTATAGTGTTAATACTAGTGTTGAGACTAGGCATTGGAGAACAGATGTTTTATGGCTTAGGGcc                                                                                                                                        <  2:119265/250‑1 (MQ=37)                                                                                                                      aaTCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAAc                                                                                                                                >  1:235997/1‑251 (MQ=255)                                                                                                                                                   catcatTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATc                                                                                                   <  1:93919/251‑1 (MQ=255)                                                                                                                                                                         aGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTg                                                                             >  1:126968/1‑251 (MQ=255)                                                                                                                                                                               tCAGATGTATAAAACATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCgg                                                                       >  1:220371/1‑251 (MQ=255)                                                                                                                                                                                 aGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCgggg                                                                     >  2:99037/1‑251 (MQ=255)                                                                                                                                                                                           aaaattaaaat‑aACCCCAAAAAACGGAATGAATAAAAATAATTTATTGTCCATTATC‑GTTATTAAGCCTGGCCCC‑TGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGtctc                                                          <  2:71036/251‑1 (MQ=11)                                                                                                                                                                                            aaattaaaattaACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGtctc                                                          <  2:136449/251‑1 (MQ=255)                                                                                                                                                                                                                     aatggagtAAAAATA‑GTTATTGTTCATTATCCGTTATTA‑GACTGGCCCCCTGAAGCTCCAGACAACCAATGTCACTTAAATAAGTGATAGTCTTAATTCTAGTATTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGCGGCGGAGAACCGCAGACGGCGGAGCGCACAGGAAAAGATCGCAATTGTTCAGAAGAGCTTTGATCCGGGGATGACGGTCGCACTCTTTGCCCTGCAACagggggta                                 <  2:181770/244‑8 (MQ=14)                                                                                                                                                                                                                                       tattGTTCATTATCCGTTATTA‑GACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTAtt                >  1:96706/1‑251 (MQ=38)                                                                                                                                                                                                                                                    ccGTTATTA‑GACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTaa    <  1:17289/250‑1 (MQ=32)                                                                                                                                                                                                                                                      gTTATTA‑GACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGATATTTCTCTGGCGTAAGc  >  2:72664/1‑250 (MQ=14)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      TCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATA‑TTTATTGTTCATTATCCGTTATTA‑GACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGC  >  NC_000913/3185847‑3186340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.