Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,293,326	IS1 (–) +9 bp	intergenic (‑404/‑193)	hns ← / → tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	257908 =	NA (NA)	18 (0.690)	18/492	0.5	94.5%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 1293334	1 (0.040)					intergenic (‑412/‑193)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
*	?	NC_000913	1293326 =	1 (0.040)	16 (0.620)	16/492	0.6	93.9%	intergenic (‑404/‑201)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
	?	NC_000913	= 1979270	NA (NA)					noncoding (1/768 nt)	IS1	repeat region

AGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGAC  >  NC_000913/257907‑258157  |                                                                                                                                                                                                                                                          aggtaATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCAGGCAGCTCCACCGATTGTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCGCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGAGTATGTGCAGCTTTCCCTTCAGGCGGGAGTCAGACAGCGGCCATCCATCCGTCAGCCATATCACCACGGCAAAGTGTGAc  <  2:134678/251‑1 (MQ=11)  |                                                                                                                                                                                                                                                          AGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGAC  >  NC_000913/257907‑258157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.