Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	458,790	IS186 (+) +6 bp :: Δ1 bp	intergenic (+90/‑93)	clpX → / → lon	ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	15387 =	NA (NA)	28 (1.090)	27/484	0.1	100%	noncoding (1/1345 nt)	IS186	repeat region
	?	NC_000913	= 458795	0 (0.000)					intergenic (+95/‑93)	clpX/lon	ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease
*	?	NC_000913	458790 =	1 (0.040)	33 (1.280)	31/486	0.1	97.0%	intergenic (+90/‑98)	clpX/lon	ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease
	?	NC_000913	= 609350	NA (NA)					noncoding (1344/1345 nt)	IS186	repeat region

CACGTAATGACATCCCCCCGGGGCCGTAAGCCAGCCCCAGACGTAGCAGAGTTGCAGCATCACGAATTTCGCGGCGGCGGGTTAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/15619‑15387 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggaTGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTTCCGGCTTGCCATAAATCAGCAACGGTTTGCTTTGACCATCAATTACCGACTCGTCGATAACCACTTTTTCGACGTCTTCCATGGACGGCAGATCGTACATGGTATCG  <  NC_000913/458795‑458568                                                                                                                                                                                                                                                                                                                                                                                                                                                                               CACGTAATGACATCCCCCCGGGGCCGTAAGCCAGCCCCAGACGTAGCAGAGTTGCAGCATCACGAATTTCGCGGCGGCGGGTTAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCA                                                                                                                                                                                                                     <  1:75759/250‑1          ACATCCCCCCGGGGCCGTAAGCCAGCCCCAGACGTAGCAGAGTTGCAGCATCACGAATTTCGCGGCGGCGGGTTAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGA                                                                                                                                                                                                            >  2:233547/1‑250                    GGGGCCGTAAGCCAGCCCCAGACGTAGCAGAGTTGCAGCATCACGAATTTCGCGGCGGCGGGTTAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGATTTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTACGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAA                                                                                                                                                                                                  >  2:189889/1‑250                             AGCCAGCCCCAGACGTAGCAGAGTTGCAGCATCACGAATTTCGCGGCGGCGGGTTAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGG                                                                                                                                                                                        >  2:158887/1‑251                                  GCCCCAGACGTAGCAGAGTTGCAGCATCACGAATTTCGCGGCGGCGGGTTAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGAT                                                                                                                                                                                   <  1:125595/251‑1                                              GCAGAGTTGCAGCATCACGAATTTCGCGGCGGCGGGTTAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCT                                                                                                                                                                       <  1:10064/251‑1                                                               CGAATTTCGCGGCGGCGGGTTAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTG                                                                                                                                                      <  1:145560/251‑1                                                                 AATTTCGCGGCGGCGGGTTAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATGAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTA                                                                                                                                                    <  1:126584/251‑1                                                                     TCGCGGCGGCGGGTTAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGG                                                                                                                                                 <  2:109773/250‑1                                                                                                 TACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGT                                                                                                                    >  1:139121/1‑251                                                                                                      GCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGC                                                                                                               >  1:14789/1‑251                                                                                                        CGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTT                                                                                                             <  2:139121/251‑1                                                                                                                CCAGTTATTCGGTCTTCCCCATATGGGGCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGGAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTGTTTGGTTAACTAATTGTATGGGAATGGTTAAGTATTCACCAGATGCCTGTTGCGCTTCCGGCTTG                                                                                                     <  2:174444/251‑1                                                                                                                  AGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTGATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAAGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAAGTGTATGGGAATGGTGAATTATTCGCCAGATGCCTGacagctggtgaataatta                                                                                                   <  2:112733/251‑19                                                                                                                                ACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTTCCGGCTTGCCATAAATCAGCAACG                                                                                     <  1:82099/251‑1                                                                                                                                      ATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTTCCGGCTTGCCATAAATCAGCAACGGTTTGC                                                                               >  1:187097/1‑251                                                                                                                                        GGGCCAGAATTGCGGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCACCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTTCCGGCTTGCCATAAATCAGCAACGGTTTGCTT                                                                             >  2:201161/1‑251                                                                                                                                         GGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTTCCGGCTTGCCATAAATCAGCAACGGTTTGCTTT                                                                            <  2:100036/251‑1                                                                                                                                         GGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTTCCGGCTTGCCATAAATCAGCAACGGTTTGCTTT                                                                            >  1:100036/1‑251                                                                                                                                           CCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTTCCGGCTTGCCATAAATCAGCAACGGTTTGCTTTG                                                                           >  1:143156/1‑250                                                                                                                                              GAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTTCCGGCTTGCCATAAATCAGCAACGGTTTGCTTTGACC                                                                        >  1:220354/1‑250                                                                                                                                               AATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTTCCGGCTTGCCATAAATCAGCAACGGTTTGCTTTGACCAT                                                                      <  1:259430/251‑1                                                                                                                                                                       GTAATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTTCCGGCTTGCCATAAATCAGCAACGGTTTGCTTTGACCATCAATTACCGACTCGTCGATAACCA                                              <  1:98694/251‑1                                                                                                                                                                         AATTCATCGGCACATTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTTCCGGCTTGCCATAAATCAGCAACGGTTTGCTTTGACCATCAATTACCGACTCGTCGATAACCACT                                            >  1:146159/1‑251                                                                                                                                                                                         AAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTTCCGGCTTGCCATAAATCAGCAACGGTTTGCTTTGACCATCAATTACCGACTCGTCGATAACCACTTTTTCGACGTCTTCC                             <  2:14789/250‑1                                                                                                                                                                                                            ACCACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTTCCGGCTTGCCATAAATCAGCAACGGTTTGCTTTGACCATCAATTACCGACTCGTCGATAACCACTTTTTCGACGTCTTCCATGGACGGCAGATCGTACAT         >  2:152344/1‑251                                                                                                                                                                                                               ACAACCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTTCCGGCTTGCCATAAATCAGCAACGGTTTGCTTTGACCATCAATTACCGACTCGTCGATAACCACTTTTTCGACGTCTTCCATGGACGGCAGATCGTACATGGT      >  1:250778/1‑251                                                                                                                                                                                                                   CCCTTAAGTTAGCGCTTATGGGATGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTGTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTTCCGGCTTGCCATAAATCAGCAACGGTTTGCTTTGACCATCAATTACCGACTCGTCGATAACCACTTTTTCGACGTCTTCCATGGACGGCAGATCGTACATGGTATCG  <  2:27907/251‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                               CACGTAATGACATCCCCCCGGGGCCGTAAGCCAGCCCCAGACGTAGCAGAGTTGCAGCATCACGAATTTCGCGGCGGCGGGTTAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/15619‑15387 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggaTGTTTCCCCCACATTCAACGCCGAGAATAGAGGAAAAATTAAAGGGGAGATAAAATCCCCCCTTTTTGGTTAACTAATTGTATGGGAATGGTTAATTATTCACCAGATGCCTGTTGCGCTTCCGGCTTGCCATAAATCAGCAACGGTTTGCTTTGACCATCAATTACCGACTCGTCGATAACCACTTTTTCGACGTCTTCCATGGACGGCAGATCGTACATGGTATCG  <  NC_000913/458795‑458568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.