Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,665	Δ1 bp	coding (384/1701 nt)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,665	0	C	.	100.0%	140.8 / NA	31	coding (384/1701 nt)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (14/17);  total (14/17)

ATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTT‑CAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATT  >  NC_000913/69431‑69910                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       gcgCCCGGCGCGGCAACGCCCGCAACGAATACCCGGCGGGGGGGGGTTACGGGAAAGCAGAGCGGCCCCCCCGTCGCCCACCGCAATCCACGATGCGGCAGATGCCGCCAGGGCGGTGTCCTGGCGGGTCACAGGCGGTATTTTTGCCCAGACCCATTCGCGGGAATAAATCCCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAg                                                                                                                                                                                                                                       <  2:257950/249‑1 (MQ=255)          cgtgaaaCGGCCGCGACGAATATCCTGCGGGCGGGTGGCACCGGAAAGCAGAGCTGCCACCCAGTCGAACAGCTCAACCCCCGAGGCGGCAGATTGCGCCACGGCGCTGTCCTGGGGAGTCACATGCAGGATTTTTGCCCAGACCCATCCGCGGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGTTCtt                                                                                                                                                                                                                              <  2:65206/246‑1 (MQ=255)              cAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTcc                                                                                                                                                                                                                           >  1:211181/1‑250 (MQ=255)                aCGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCcac                                                                                                                                                                                                                         >  2:212759/1‑250 (MQ=255)                  gTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAAt                                                                                                                                                                                                                      >  2:65023/1‑251 (MQ=255)                       ggacgaATATCCTGCGGGCGGTGGGTACCGGAAAGCAGAGCGGGCACCAATTCGCACAGACAAATCAACGTGGCGGAAGATTGCCCCACGGCGCTGTCCTGGCGGGTAACATGAAGGATTTTTGCCCAGAACCATTCGCTGGAATAAACACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGaa                                                                                                                                                                                                                 <  1:233659/250‑1 (MQ=255)                       cgacgaATAGCCGGGGGGGGGGTGGGACCGAAAAGCAGAGCTGGCCCCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGaa                                                                                                                                                                                                                 <  2:95784/251‑1 (MQ=255)                                 ccTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTc                                                                                                                                                                                                       >  1:84797/1‑251 (MQ=255)                                                   cGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCCCGAGGCGGCAGATTGCGCCACGGCGCTGCCCTGGGGATTCACAGGCAGGATTTTTGCCCAGACCCATGCGCTGAAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTGCGGCAAACTcc                                                                                                                                                                                     <  1:202000/251‑1 (MQ=255)                                                        aGCAGAGCTGGCCCCCAGTCGCACAGCTCAATCCACGATGCGGCAGATGCCGCCCCGGCGCTTTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGACCCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGgcg                                                                                                                                                                                <  1:153864/251‑1 (MQ=255)                                                                                          ccgaggCGGCAGATTGCCCCACGGCGCTGTCCGGGCGATTCACAGGCAGGATTTTTGCCCAAACCCATTCGCTGAAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGt                                                                                                                                              <  1:10732/246‑1 (MQ=255)                                                                                              tGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCgg                                                                                                                                          >  2:142599/1‑251 (MQ=255)                                                                                                                ggCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGt                                                                                                                        >  1:38755/1‑251 (MQ=255)                                                                                                                     tGTCCTGGCGAGCCAAATGATGGTTTTTTGCCAAGAACCTTACCCTGAAAAAAACTCCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAAc                                                                                                                    <  2:18064/250‑1 (MQ=255)                                                                                                                             cGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATccc                                                                                                           >  1:92513/1‑251 (MQ=255)                                                                                                                                     aTGCAGGATTTTTGCCCAGAACCATTCGCTGGAAAAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAg                                                                                                   <  1:65023/251‑1 (MQ=255)                                                                                                                                               tttGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGAGGGAGTAGTCAACATTGCAAGGCGCGTGGCACAACCGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCCCAATACGAACATCGCGTTCGGGCTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACACCAGCTGCGCGCTg                                                                                         >  1:125842/1‑251 (MQ=255)                                                                                                                                                  gccaagaacatttCGCTGAAAGAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCTAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGtt                                                                                       <  1:179603/239‑1 (MQ=255)                                                                                                                                                             ttCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTAAGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGct                                                                           >  1:136312/1‑251 (MQ=255)                                                                                                                                                                  gggAAAAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGGCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCa                                                                      <  1:10892/250‑1 (MQ=255)                                                                                                                                                                   gaaataaataCCGCCAATTTAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCGCGTTACCGTCGGCATCAATCGGTGCGGGCGTCGGGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTTTTCGACGCTAAGCTCTGCa                                                                      <  1:70613/248‑1 (MQ=255)                                                                                                                                                                   gaaaaaaatcccgccaATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCa                                                                      <  2:84797/237‑1 (MQ=255)                                                                                                                                                                     aataaataACGCCAATATAGCGGGAGCGGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGACTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGTGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGc                                                                   >  2:102407/1‑251 (MQ=255)                                                                                                                                                                                    tatAGCGGGAGCAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GATTCTTTCAACCGCAGGGTGGTCTTTCAACAATACGAACATCGCGTTCGGGTATTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGAGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCCGCGCGCTGTTCGACGCTAAGATCTGCAAGCACGGTTTTCAGTGcg                                                    >  2:237768/3‑250 (MQ=255)                                                                                                                                                                                                    aacattGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCa                                     >  2:234338/5‑250 (MQ=255)                                                                                                                                                                                                     acattGCCCGGCGCGTGGCAAAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGACGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGTTTTTCGGCAAACTCAGGGCGCAGCGCCAACACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAAt                                   >  2:214938/4‑251 (MQ=255)                                                                                                                                                                                                            ccGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCTTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAATCACGGTTTTCAGTGCCGCTTCCATTGCCTCAATGTAGTCa                            <  2:20760/251‑1 (MQ=255)                                                                                                                                                                                                            caggcgcgcagCACAAACGGGTAATATCTTA‑GCTTCTTTCACCGGCAGTGTGGTCTTTCCACAATACGAACATCGCGATCGGCTTTCCGGCAAACTCAGGGCGCAGCTCCAGCACGTTACCGTCGGCATCAATCGGTGCGGCCGTCGAGCCGGTAATGTCAACGCCAATCCCGACCACAGCTGCGAGCTGTTCGACGCTAACCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCATTGTAGTc                             >  2:6210/11‑250 (MQ=255)                                                                                                                                                                                                                     ggCACAAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGgatg                    <  1:191692/250‑1 (MQ=255)                                                                                                                                                                                                                       cacaAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGatgatg                 <  2:97976/251‑1 (MQ=255)                                                                                                                                                                                                                         caAACGGGTAATCTCTTC‑GCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCCCGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGAc               <  1:138188/251‑1 (MQ=255)                                                                                                                                                                                                                                          tcGCTTCTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTAtt  <  1:102096/247‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       ATGCCCGGCGCTGCAACGTCCGCGACGAATATCCTGCGGGCGGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTTCTT‑CAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATT  >  NC_000913/69431‑69910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑