Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,549,037	T→C	V77A (GTC→GCC)	fimH →	type 1 fimbriae D‑mannose specific adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,549,037	0	T	C	100.0%	33.0 / NA	12	V77A (GTC→GCC)	fimH	type 1 fimbriae D‑mannose specific adhesin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/5);  total (7/5)

GTTTGCTGTACTGCTGATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCT‑AATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTAT  >  NC_000913/4548828‑4549281                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      gTTTGCTGTACTGCTGATGGGCTGGGCGGTAAAGGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCt                                                                                                                                                                                                               <  1:201714/250‑1 (MQ=255)                                             tCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCCGCGCCCATGTTTATGTAAACCTTGCTCCCGCCGTGAATGTGGGGCAAAACCTGGGCGTGGGTCTTTCGACGCAAATCTTTTTCCATAAAGATTATCCGGAAACCATTACAGACTATGCCCCCCTGCAACGAGGCTCCGCTTATTGCGGCGCGTTTTTTCATTTTTTCCGGGCCCG‑CAAATATAATGGCAggatgtttaccttt                                                                                                                                                                 >  2:164892/1‑238 (MQ=255)                                                                       gCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCT‑AATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCgt                                                                                                                                       <  2:79145/251‑1 (MQ=255)                                                                                                                           tcgtGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCT‑AATTTTTCCGGGACCGGAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGAAAAGCCGTGGACGGGGGCGCTTTTTTTTACg                                                                                   >  2:187757/1‑251 (MQ=255)                                                                                                                                         cAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCT‑AATGTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCTGTGGCGCTTTATTTGACGCCTGTGAGCAGTgc                                                                     <  2:130342/251‑1 (MQ=255)                                                                                                                                                                gACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCCCGGCTTATGGCGGCGTGTTATCT‑AATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCCCCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTg                                              >  1:96538/1‑251 (MQ=255)                                                                                                                                                                        tCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCT‑AATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCAtt                                       >  1:47461/1‑250 (MQ=255)                                                                                                                                                                           tttGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAAGCTCGGCTTATGGCGGCGTGTTATCT‑AATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTa                                      <  1:110898/248‑1 (MQ=255)                                                                                                                                                                              gCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCT‑AATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCCGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCCTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTCAAGCTGGCTCATTAATTGcc                                >  1:91896/1‑251 (MQ=255)                                                                                                                                                                                              ggAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCT‑AATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGc                                                                                                       >  1:82503/1‑164 (MQ=255)                                                                                                                                                                                              ggAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCT‑AATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGc                                                                                                       <  2:82503/164‑1 (MQ=255)                                                                                                                                                                                                             cTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCT‑AATTTTTCCGGGACCGTAAAATATAGTGGAAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCTTTAATTGCCGTGCTTATTTTGCGACAGACCAACAACtat  >  1:221587/1‑250 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      GTTTGCTGTACTGCTGATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCT‑AATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTAT  >  NC_000913/4548828‑4549281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑