Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,396,772	T→C	F236F (TTT→TTC)	amiB →	N‑acetylmuramoyl‑L‑alanine amidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,396,772	0	T	C	96.2%	83.1 / NA	26	F236F (TTT→TTC)	amiB	N‑acetylmuramoyl‑L‑alanine amidase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (17/8);  minor base G (0/1);  total (17/9)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

GCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAA  >  NC_000913/4396535‑4397017                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      gcgcAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTaa                                                                                                                                                                                                                                          <  1:35216/251‑1 (MQ=255)       tCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGAGTACTTTGCTCAATGACGATCCGATGTTCAAAGGAGTTTTAACCCggg                                                                                                                                                                                                                                    >  1:62844/1‑249 (MQ=255)         cGTTTAAAACGGAAAGTAACCGACCTACGGGTGTTATCAGCAGTAATACGGTAACGCGGCCGGCAGCGCGCGCGAAGGCTAACACTGGCGATAAAATTATCTTCGATATTGATGCCGGACACGGCGATCAGGATCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGGAAATTGAGTACTTTGCTCAAGGACGATCCGATGGTGAAAGGCGTTTTAATCCGTGAc                                                                                                                                                                                                                                  <  2:163086/251‑1 (MQ=255)               aaaCGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCGCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGAt                                                                                                                                                                                                                            <  2:96800/251‑1 (MQ=255)               aaaCGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTAAGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCCAAGGCGTTTTTACCCGTGACGGGGAt                                                                                                                                                                                                                            >  2:75530/1‑251 (MQ=255)                                aCTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCCTCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGAt                                                                                                                                                                                                           >  1:173535/1‑251 (MQ=255)                                                             aaCGCGTCCGGCCGCGCGCGCGACGGCTAACACTGGCGCTAAAATTATCATCGCTATTGATGCCGGACACGCCGGTCCGGACCCTGGCGCTATCCGCCCCGGTGGTACGCGGGAGAAAAATGTCACCCTCGCCATCGCCCGTAAATTGCGTCCTTTGCCCAATGACGCTCCGATGTTCACAGGCGTTTTAACCCGCGACGGGGATTACTTTATCTCGGTGATTGGGTGCAGTTATGTGGCACGTAAGCaa                                                                                                                                                                               >  2:28588/1‑250 (MQ=255)                                                                    ccGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCaa                                                                                                                                                                       <  1:142151/251‑1 (MQ=255)                                                                                   aCGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCCGGACCCTGGCGCCATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGGGATGGGGCGCAGGGATGTGGCACGGAAACAAAACGCCAATTTCCTCGTGTCgtt                                                                                                                                                        >  2:215775/1‑249 (MQ=255)                                                                                                      aaaTTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCg                                                                                                                                     >  1:64199/1‑251 (MQ=255)                                                                                                                 gCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGc                                                                                                                          >  1:105220/1‑251 (MQ=255)                                                                                                                           ccGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCt                                                                                                                <  1:126575/251‑1 (MQ=255)                                                                                                                             ggACACGGCGGTAAGGACCCTGGCGCTATCGGCCCCGGGGGGACGCGGGAGAAAAATGTCACCATCGCCCTCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTTTCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCCATTTCCTCCTGTCGATTCACGCTGTTGCCGCACCGAACCGCAGTGCGACTGGCGCTTc                                                                                                              >  2:227392/1‑251 (MQ=255)                                                                                                                                  cggcggTCAGGACCCTGGCGCTATCGGCCCCGGCGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATTCCGCACCGAACCGCAGGGCGACTGGCGCTTCCGTAt                                                                                                         >  2:113724/1‑251 (MQ=255)                                                                                                                                                  ggCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAAcc                                                                                          <  1:53584/250‑1 (MQ=255)                                                                                                                                                     gcTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTcg                                                                                      >  2:181598/1‑251 (MQ=255)                                                                                                                                                                         gcgGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGcc                                                                   >  2:75532/1‑250 (MQ=255)                                                                                                                                                                              agaAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCACTGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCACCAGTGAAATGGCCAgctgg                                                             >  1:201846/1‑251 (MQ=255)                                                                                                                                                                                aaaaaTGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAgctggct                                                           <  1:7162/251‑1 (MQ=255)                                                                                                                                                                                   aaTGTCACCATCGCCATCGCGCGTAAATTGCGTACCTTGCTCACTGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGCTGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGctggctggg                                                        >  1:91498/1‑250 (MQ=255)                                                                                                                                                                                        cACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGagcagc                                                   >  1:224647/1‑251 (MQ=255)                                                                                                                                                                                                             aTTGCGTACCTTGCTCAATGACGATCAGATGTTCAAAGGCGTTTTAAAACGTGACGGGGATTACTTTATCTCGGAGATGGGGTGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGCTGCCGCACCGAACAGCAGTGCGCCTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGATGGCTGGAGTAGCCCGAGAAACAGTCGGAgctgc                              >  1:157208/1‑251 (MQ=255)                                                                                                                                                                                                               tGCGTACTTTGCTCAATGACGATCCGATGTGCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAgctgct                             <  2:100389/250‑1 (MQ=255)                                                                                                                                                                                                                gCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGctgctg                            <  1:13819/250‑1 (MQ=255)                                                                                                                                                                                                                          cTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCggg                 >  1:50372/1‑251 (MQ=255)                                                                                                                                                                                                                                          tGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGGGGTGGGCTGGCGaa  >  2:191623/1‑250 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      GCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAA  >  NC_000913/4396535‑4397017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑