Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,393,552	G→A	F172F (TTC→TTT)	queG ←	epoxyqueuosine reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,393,552	0	G	A	100.0%	108.5 / NA	33	F172F (TTC→TTT)	queG	epoxyqueuosine reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/13);  total (20/13)

ATAAGCTGGCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGTTTGGGGTTTTTCAGCGTGC  >  NC_000913/4393313‑4393796                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     aTAAGCTGGCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAAcc                                                                                                                                                                                                                                           >  1:186854/1‑251 (MQ=255)    aGCTGGCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGc                                                                                                                                                                                                                                        >  1:52140/1‑251 (MQ=255)                                  tttCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCCCGGCATTGAGGGTAAGTGAGGGCTTAcc                                                                                                                                                                                                          >  1:136028/1‑251 (MQ=255)                                     cccATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGt                                                                                                                                                                                                       >  1:200325/1‑251 (MQ=255)                                                                aTCGCCCCTTCACGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTa                                                                                                                                                                             >  2:137998/1‑250 (MQ=255)                                                                 ccGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAAc                                                                                                                                                                           <  2:22179/250‑1 (MQ=255)                                                                            aGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCgag                                                                                                                                                                <  1:1471/251‑1 (MQ=255)                                                                                     gTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGAGTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGgcgc                                                                                                                                                       <  1:111041/251‑1 (MQ=255)                                                                                               agaTACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGAACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGAc                                                                                                                                              >  1:204260/1‑250 (MQ=255)                                                                                                         gcgAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTa                                                                                                                                    >  1:174374/1‑250 (MQ=255)                                                                                                              gaTCGACGTTATATGGCGCGACGATGGCGCCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGTGGAGAAGTGAGTGCTGGCCTGTTCAGTCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCTAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTc                                                                                                                              <  1:141010/250‑1 (MQ=255)                                                                                                                                  acgaTGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTg                                                                                                          <  2:82724/251‑1 (MQ=255)                                                                                                                                        gCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAGCACAATGTTGCTGAatcat                                                                                                    <  2:194093/251‑1 (MQ=255)                                                                                                                                          aCCGGTCGGGCAAATCGTCATACAGGCCCCGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGACTATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATACGTGAGCGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCAtct                                                                                                  >  2:116145/1‑251 (MQ=255)                                                                                                                                             ggacGGGCAAATCGTCATACAGGCCCCGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAACATTCAGCGAAACACAATGTTGCTGAATCATCTCGc                                                                                               >  2:14376/4‑251 (MQ=255)                                                                                                                                                                cAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGtt                                                                            >  1:42968/1‑251 (MQ=255)                                                                                                                                                                 cggCCACTCATTTGCCCCCTCCTTCCTCGACTGTTTGTGCCACGGGCAGCGGAATATCGACATGCAATTGGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGAAAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGGCAAACTGTCTAAAAGTCAGCGAAACACAATGTTGCTGAATCAGCTCGCCCAGCTTTTTGTGTCGGTTg                                                                           <  2:195126/250‑1 (MQ=255)                                                                                                                                                                    ccACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGCTAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTgccc                                                                        >  2:228072/1‑249 (MQ=255)                                                                                                                                                                    caaCGCTTTTGCCGCATCCTTCCTCGACTGGTTGATCAACGGGCAGCGGAATTTCGACCAGAAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGTGATTGAGGATAAGTGAGTGATTACCAGTCCAGCCGAGCCAAGCTTTTTCAGCTAACGGGCGCTGGAGCATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCCGAATCATCTCGCACAGATTTTTGAGTCGGATgctc                                                                        >  2:228076/3‑249 (MQ=255)                                                                                                                                                                                 gCATCCTTCCTCGACTGGTTGATCCACGGGCAGAGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGAt                                                           <  2:177086/251‑1 (MQ=255)                                                                                                                                                                                             gacaggatGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCAGACCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTATAAAATTCATCGAACCACAATGTTGCTGAAACATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAACAGATTGTGATACTCACGcccc                                                >  2:170292/8‑250 (MQ=255)                                                                                                                                                                                                   ttGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGaa                                                                                                                                                   <  2:79895/145‑1 (MQ=255)                                                                                                                                                                                                   ttGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGaa                                                                                                                                                   >  1:79895/1‑145 (MQ=255)                                                                                                                                                                                                     gATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATa                                        >  1:226778/1‑250 (MQ=255)                                                                                                                                                                                                         cACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGACGTTTGTGATAGTCACGGCCCAGCGCATAACGGc                                   >  2:181518/1‑251 (MQ=255)                                                                                                                                                                                                          aCGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGTCGCAGAATCGACTAACGGTCTAAAATTCAGCGAAACACAATGTTGTTTAATCATCGCGCCCAGCTTTTTGAGGCGGTGGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCt                                  <  1:143434/251‑1 (MQ=255)                                                                                                                                                                                                           cGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGTTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTa                                 <  1:136598/251‑1 (MQ=255)                                                                                                                                                                                                           cGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCt                                  >  1:223384/1‑250 (MQ=255)                                                                                                                                                                                                                                   aTTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGTTTGGGGTTTTTc         <  2:59458/251‑1 (MQ=255)                                                                                                                                                                                                                                   aTTCGCCTAAAAAAAAGAACGAACCGGCATCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGTTTGGGGTTTTTc         >  1:21317/1‑251 (MQ=255)                                                                                                                                                                                                                                    ttCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGc                                                                                               <  2:2598/164‑1 (MQ=255)                                                                                                                                                                                                                                    ttCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGc                                                                                               >  1:2598/1‑164 (MQ=255)                                                                                                                                                                                                                                          tAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGTTTGGGGTTTTTCAGCGTGc  >  2:102744/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     ATAAGCTGGCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGTTTGGGGTTTTTCAGCGTGC  >  NC_000913/4393313‑4393796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑