Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,148,972	C→T	Q72* (CAG→TAG)	fabH →	3‑oxoacyl‑[acyl carrier protein] synthase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,148,972	0	C	T	100.0%	96.4 / NA	29	Q72* (CAG→TAG)	fabH	3‑oxoacyl‑[acyl carrier protein] synthase 3
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/10);  total (19/10)

AACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCGATGTACTGGCGCGCACCTGCGATCCAACCGA  >  NC_000913/1148736‑1149207                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            aaCCGACAAGAGACTGAGCGTCCATGTATACGAAGATTATTGGTACTAGCAGCTATCTGCCCGAACAAGTGCGGACAAAAGCCGATTTGGAACACATGGTGGAAACCTCTGACGAGTGGATTGTCACTTGTACCGGTATCCGCGACCTCCACATTGCCGCGCCAAACGCAACCGTTTCACCCATGGGCTTTGACGATGCGACACGCGAAATTGAGTTGGCGTGCCTTGAGAACGACTAGCTTGGcgtactc                                                                                                                                                                                                                               >  2:78449/1‑245 (MQ=255)         aGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCAGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGAAACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGc                                                                                                                                                                                                                       >  1:62322/1‑251 (MQ=255)                               cGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCt                                                                                                                                                                                                 >  1:76011/1‑251 (MQ=255)                                gAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACCAAAGCCGATATGGCCAAAATGGTGGACACCTCTCCCGAGTGGATTGTAACTCGCACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGAATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTCCGCACGCtt                                                                                                                                                                                                >  1:134371/1‑251 (MQ=255)                                gAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCtt                                                                                                                                                                                                >  1:118957/1‑251 (MQ=255)                                                                aaCAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGCCACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATg                                                                                                                                                                >  1:158273/1‑251 (MQ=255)                                                                aaCAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATg                                                                                                                                                                >  2:62848/1‑251 (MQ=255)                                                                   aaGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTg                                                                                                                                                             >  1:5142/1‑251 (MQ=255)                                                                       gCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGc                                                                                                                                                          <  1:198892/250‑1 (MQ=255)                                                                                   cGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGcc                                                                                                                                             >  1:170204/1‑251 (MQ=255)                                                                                                         cccTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCagcagc                                                                                                                       <  1:148910/250‑1 (MQ=255)                                                                                                              tGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTgcg                                                                                                                  >  1:168845/1‑251 (MQ=255)                                                                                                                    gTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGATTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAggat                                                                                                            >  2:227220/1‑249 (MQ=255)                                                                                                                    gTGGATTGTCACTCGTACCGGTAGCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTGCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGACGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGTGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTGGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGtt                                                                                                            <  2:110675/251‑1 (MQ=255)                                                                                                                      ggATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTc                                                                                                          <  2:62940/251‑1 (MQ=255)                                                                                                                             cACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAAGTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTTGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTAt                                                                                                    <  1:93922/250‑1 (MQ=255)                                                                                                                                 cGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCAtt                                                                                               >  1:141212/1‑251 (MQ=255)                                                                                                                                  ggaccagTATCCGCGAACGCCACATTGACGCGCCATACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGCTCGTTGTGGCAACGACTTCTGCTAAGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTACAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCAttt                                                                                              >  2:36762/7‑250 (MQ=255)                                                                                                                                        gTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTa                                                                                        <  2:199833/251‑1 (MQ=255)                                                                                                                                                 aaCGCCACATTGCACCGCCAAACGACACCGTTTCAACCAAGGGCTTTGACGCGGCGACACGCGCAATTGAGATGGAGGGCATTGAGAAAGCCTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGCCGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCat                                                                               >  2:38319/1‑250 (MQ=255)                                                                                                                                                            gCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATc                                                                    >  1:122283/1‑251 (MQ=255)                                                                                                                                                              cgcgCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTg                                                                  <  1:46729/251‑1 (MQ=255)                                                                                                                                                                          aaCCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATAGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGCCAGAGTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTCGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGACGATCAATACGTGGAATCTGGGGCGGTGaatt                                                      >  2:135718/1‑249 (MQ=255)                                                                                                                                                                                ttCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGct                                                 <  2:10029/250‑1 (MQ=255)                                                                                                                                                                                          ggCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGAATAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCgg                                      >  1:21581/1‑251 (MQ=255)                                                                                                                                                                                                 aaGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCGAt                               >  1:37158/1‑251 (MQ=255)                                                                                                                                                                                                                 aaTTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCAGTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCGCTGGTCGTCGGTTCCGATGGACTGGCGCGCACCt               <  2:101750/251‑1 (MQ=255)                                                                                                                                                                                                                         tGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCGATGTACTGGCGCGCACCTGCGCTcc        >  1:160758/1‑250 (MQ=255)                                                                                                                                                                                                                              ggCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCGATGTACTGTCGCGCACCTGCGATCCAACCGa  <  2:204405/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            AACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCGATGTACTGGCGCGCACCTGCGATCCAACCGA  >  NC_000913/1148736‑1149207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑