Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,359,314	G→A	Q240* (CAG→TAG)	cadB ←	lysine:cadaverine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,359,314	0	G	A	100.0%	75.5 / NA	22	Q240* (CAG→TAG)	cadB	lysine:cadaverine antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/13);  total (9/13)

TTCGGAATACCGTTGCTGTCGACTTCACCATAAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCA  >  NC_000913/4359073‑4359549                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            ttctgAATACCGTTGCTGTCGACTGCGCCATAAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCATTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGATGCCGCGATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAg                                                                                                                                                                                                                                     <  1:97752/246‑1 (MQ=255)          ccGTTGCTGGCGACTTCACCATAAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCCCCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAAc                                                                                                                                                                                                                           <  2:164013/251‑1 (MQ=255)                              aTAAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGCAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTAc                                                                                                                                                                                                       >  2:4986/1‑251 (MQ=255)                                 aaCTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTAccc                                                                                                                                                                                                     >  1:57063/1‑250 (MQ=255)                                    tttCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTAccccgcc                                                                                                                                                                                                 >  1:162705/1‑247 (MQ=255)                                       cGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTAACCAGCATgg                                                                                                                                                                                              >  2:205998/1‑251 (MQ=255)                                            aggtACCGTGGTTAGCGGCACGTAAACGTGCCTGGCCTACCAACATCAGACAGGAGCCCAGAGAAGGCAGGCACGCAAAGGCGGTGAAGGCAGAAACCAGCGGCGCAGCCCATTTACCGAGTATAGTTGAAGCACTGATTACAAAGGGAGCCCAGGAAGCCGACAGTACAGAAAACGGATACATACCGGAAAGCCCCTAAGTCGCAGCGATGTAAACAATAACTGCTAAACCAGTACCCAGCATGGTTGcc                                                                                                                                                                                         <  2:11053/248‑1 (MQ=255)                                             gTTACCGTCGTTAGCGGCAGGTACATCTGCCTGGCATACCAACATCCTCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGGGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACGGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGGTAAACCAGTACCCAGCATGGTTGCCa                                                                                                                                                                                        <  2:137590/251‑1 (MQ=255)                                                                    cacCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGGGAAGGCAGGCAGGCAAAGGCGGTGAATGCAGAAACCGGCGGCGCAGCCCGGTTCCGGAGGAGAGTTTAAGCGCTGATTGCAAACGTAGCACCGGAGGTCGCCATTACAGAAGACGGATATATACCGGAAAGCTCCGAAGTCTCAGCGATGTAAACAGGAGCTGGTAAGCCATGGCCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGttt                                                                                                                                                                 <  1:224479/251‑1 (MQ=255)                                                                      cctgcctgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGttttt                                                                                                                                                               <  1:37279/251‑1 (MQ=255)                                                                                  aCCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGt                                                                                                                                                   >  2:56133/1‑251 (MQ=255)                                                                                               aGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGCGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCg                                                                                                                                      >  1:91069/1‑251 (MQ=255)                                                                                                        gagaAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGTAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAac                                                                                                                              >  1:198696/1‑250 (MQ=255)                                                                                                                         aaGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGCTTCAACACCCACGAAGGCCcac                                                                                                              >  2:85855/1‑249 (MQ=255)                                                                                                                             cGGTGAATGCAGAAACCAGCGGCGCGGCCCGGTTTCCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCTCGAAGGCCCACAGgcag                                                                                                        <  2:165115/251‑1 (MQ=255)                                                                                                                                                cggcgCAGCCCAGTTACCGAGGATAGTTGAAGCACGGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGa                                                                                     <  2:178190/251‑1 (MQ=255)                                                                                                                                                                 cGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTg                                                                    <  1:180114/251‑1 (MQ=255)                                                                                                                                                                                        gCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGc                                             >  1:67724/1‑251 (MQ=255)                                                                                                                                                                                        ccAAACTGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGTGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGTCAGAGCACAATACTTTTAATGATCGCATGACCATCAGTTGTATCCGCAGTATTCCAGTTAGc                                             <  2:118766/250‑1 (MQ=255)                                                                                                                                                                                                              ccATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGAACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAAc                       <  2:162705/251‑1 (MQ=255)                                                                                                                                                                                                                      gaagaCGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATgcca               <  1:156707/251‑1 (MQ=255)                                                                                                                                                                                                                                    acCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCa  <  1:56133/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            TTCGGAATACCGTTGCTGTCGACTTCACCATAAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCA  >  NC_000913/4359073‑4359549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑