Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,223,6380TC93.8% 44.6 / ‑6.0 16intergenic (‑10/‑190)iclR/metHDNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (5/10);  total (5/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

AACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCA‑TGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTG  >  NC_000913/4223401‑4223851
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                     
aaCCGGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCA‑TGACAGTCTCCTTTTTCTGTATCg                                                                                                                                                                                                          <  2:210392/251‑1 (MQ=255)
      aCGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTTGCAACGGCGGGTTTTCTGCTGCGTTTCGCGGGAATGGGTGCGACCA‑TGACAGTCTGCTTTTTCTGTATCGTGGaaa                                                                                                                                                                                                    <  2:27402/251‑1 (MQ=255)
                       gTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCA‑TGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTAt                                                                                                                                                                                    <  1:228099/250‑1 (MQ=255)
                                           cGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGTCACGCGTTAAAGACTGATCCTGTGCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCGGCGTTTCGCTTGAATGGGTGCGACGA‑CGACAGTCGCTTTTGTCTGTATCGCGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTa                                                                                                                                                               <  1:100773/251‑1 (MQ=255)
                                            ggTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCA‑TGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTac                                                                                                                                                              <  2:34140/251‑1 (MQ=255)
                                                                         ggttgCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCA‑TGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAAc                                                                                                                                 <  2:170147/250‑1 (MQ=255)
                                                                          gttgCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCA‑TGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTTTTAGCTAATGCAATAGTTACTAACCTGATCCGATGAGTTAATGTTGAAc                                                                                                                                 <  2:181440/250‑1 (MQ=255)
                                                                          gttgCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCA‑TGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAAc                                                                                                                                 <  1:89402/250‑1 (MQ=255)
                                                                                           aGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTTCCGCGTTTCGCGGGGATGGGGGCGGCCA‑TGACAGTCTCCCTTTTTTGTATCTTGGGAATCATTTTTTTTTTTTTTGTTTGCTAATGCAATAGTTACTGAAATGATCCGATGAGTTTATGTTGTACAAATCTCATGTTGCgtgg                                                                                                               >  1:126479/1‑251 (MQ=255)
                                                                                                                        ccACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCA‑TGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTAt                                                                                   <  2:174670/250‑1 (MQ=255)
                                                                                                                                        aGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCCCGTTTTGCGGGAATGGGGGGGACCAATAAC‑GTCTCCTTTTTTTTTATTGTGGGAATCATTTTTATTTTTTTTGTTATCTAATTCAATAGTTACTGAAATGATTCGATGAGGTTATGTTTAACAAAACTCATGTTGTGTGGGGGTTGCCTTTAACCCAGATGTGTTTATGACAGTATgggtttttg                                                                  >  1:111509/1‑244 (MQ=255)
                                                                                                                                                        aCTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTTTCGCGCTTCTCGGGGATGGGGGCGGCCC‑TTACAGTCTCCCTTTTTTTTATCGTGGGAATCATTTTTTTTTTTTTTTTTTGCTAATGCAATAGTTTCTGTACTGATCCGCTGTGTTTATGTTTAACAAATCTCATGTTTCGTGGGGGTCGCTTTTTCCCCAGATGCGTTTTTGCCTGTATGTTTTTTTGTATTTTTTTTTAATCt                                                  >  1:160086/1‑251 (MQ=255)
                                                                                                                                                                    cAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCA‑TGACAGTCTCCTTTTTCTTTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGGTTGTTGAATTTTTATTAAATCTGGGTTGAGCgtg                                      <  1:204325/251‑1 (MQ=255)
                                                                                                                                                                      gTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGGATTAGGGCGACCA‑TGACCGTCTCCTTTTTTTGTATTGTGGAAATTATTTTCATTTTTTTTGTTAGCTTATGCCATAATTTCTGAACTTAACCGCTTAGTTAATGTTGAACAAATCTCATGTTTCGGGGTGGTCGCTTTTACCACCGATTCGTTTATGCACGGATGGTTTGTTTCATTTTTTTTAAATTTGGGTTGAGCgttgt                                    >  1:90830/1‑248 (MQ=255)
                                                                                                                                                                                            cggGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCA‑TGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGCGCAAGTGTGAGCAGCaa               >  2:164696/1‑250 (MQ=255)
                                                                                                                                                                                                        cgcgTTTCGCGGGAATGGGTGCGACCA‑TGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAGTCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtg  <  2:160086/251‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                     
AACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCA‑TGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTG  >  NC_000913/4223401‑4223851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑