Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,703,531 G→T Y106* (TAC→TAA)  dppD ← dipeptide ABC transporter ATP binding subunit DppD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,703,5310GT95.2% 64.5 / NA 23Y106* (TAC→TAA) dppDdipeptide ABC transporter ATP binding subunit DppD
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (12/8);  total (14/9)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

CCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATC‑GCTTCCATAATCTGGAAACCCAC‑GGTGTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATC‑A‑TCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGT  >  NC_000913/3703296‑3703766
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             
ccAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTTATGCACCTTAATC‑GCTTCCATAATCTGGAAACCCAC‑GGTTTAGCACGGGTTAAgg                                                                                                                                                                                                                                <  1:210183/251‑1 (MQ=255)
    cgcgGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCCATCGCTCGCTGACGGCGGGGACTTTTTTTGGCGCCCCGATGCCCCTTAATC‑GCTTCCCTAATCTGGTAACCCAC‑CGTTTAGAACGGGGTTAGGCTgg                                                                                                                                                                                                                            >  1:132118/1‑251 (MQ=255)
             cGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATC‑GCTTCCATAATCTGGAAACCCAC‑GGTTTAGCACGGGTTAAGGCTGGTCATCGGGt                                                                                                                                                                                                                   <  1:205800/251‑1 (MQ=255)
                                                 aaTCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCGGGTCAATCGCTCGCTGACGGCGGGTAATTTTGTTGCCCCCCTGATGCCCCTTTATT‑GCTTCCCTAATCTTGGAACCCAC‑GGGTTAGCACGGGGTTAGGGTGGTCATCGGGGCCTGGGAGATC‑C‑TCGcccactctgcccccccccggg                                                                                                                                                                             >  2:94937/1‑232 (MQ=255)
                                                  aTCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTTGCGCCCCGCTGCACCTTAATC‑GCTTTCACAATCTGGGAACCCAC‑GGTTTAGCACGGGATAAGGCTGGGTATAGGGTCCTTGAAGA‑C‑ACACGACCCTTCGGcacccaccccggt                                                                                                                                                                            >  1:125888/1‑246 (MQ=255)
                                                      ccATGGCGATCATCAACCGCTGGCTAATGCCGCCGGCAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTTTTGCCGCCCTGGTGCACCTTAATC‑GATTCCCTAATCTGGAAACCCCC‑GGTATAGCAAGGGTTAAGGCTGGGCATAGGGTCCTGGGAGGTCCA‑T‑ACCACCTCGCCACCCACCAGGGTgcgg                                                                                                                                                                        >  2:135829/1‑251 (MQ=255)
                                                           gCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAACCATCCAGACGCGATGCCGGATCGGGAATACCGCCCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGAATTTTTTTTCCGCCCTGCTGCACCTTAATCTCCTTCC‑TAATCTTGAAACCCCCCGGT‑TAGGTCGGGGTCAAGCTGGTCACCGGGTCCTTGCAGATC‑A‑TAGCCACCTCGGCCCccaccacggtggggccccc                                                                                                                                                                   >  2:19978/1‑238 (MQ=255)
                                                            cGATCATCACGCGCTGGCTCAGGCCGCCGGAAAGCTGATGCTGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTGGTTGCCGCCCTGATGCACCTTAATC‑GCTTCCATAATCTGGAATCCCAC‑GGTGTTGCACGGGTTTAGGCTGGTCATCGGGTCCTGGAAGATC‑A‑TCGCCGCGTCGGCACCCACCAGGTTGCGGCGctct                                                                                                                                                                  <  2:133038/251‑1 (MQ=255)
                                                                catcaCGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATC‑GCTTCCATAATCTGGAAACCCAC‑GGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATC‑A‑TCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCttttt                                                                                                                                                              <  2:71896/251‑1 (MQ=255)
                                                                               cATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATC‑GCTTCCATAATCTGGAAACCCAC‑GGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATC‑A‑TCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGc                                                                                                                                                <  1:97177/250‑1 (MQ=255)
                                                                               cATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGACTTTTGTTGTCGCAATGATGCACCTTAATC‑CCTTCCCTAATCTGGAAACCCAA‑GGGTTAGCACGGGTTAAGGATGGTCATCGGGTCCTGGAAGATC‑A‑TCGCCACTTTGGCCCCCACCCGGTTGCGGGGCTCTTTTTCTTAGATAAGCTGc                                                                                                                                                >  1:24726/1‑250 (MQ=255)
                                                                                     gccgGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTTCCGCCCTGATGCACCTTAATC‑GCTTCCCTAATCTGGAAACCCAC‑GGGTTAGAACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATC‑A‑TCGCCACTTCGGCCCCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCCAATCCt                                                                                                                                         >  1:206447/1‑251 (MQ=255)
                                                                                                      ggTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTGAATC‑GCTTCCATAATCTGGAAACCCAC‑GGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATC‑A‑GCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGt                                                                                                                        <  2:110514/251‑1 (MQ=255)
                                                                                                       gTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCGGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCGTAATC‑GCTTCCATAATCTGGAAACCCAC‑GGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATC‑A‑TCGCCACGTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGtt                                                                                                                       <  2:205613/251‑1 (MQ=255)
                                                                                                                   aCGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATC‑GCTTCCATAATCTGGAAACCCAC‑GGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATC‑A‑TCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTa                                                                                                           <  1:177511/251‑1 (MQ=255)
                                                                                                                                                        agGTCAATCGCTCGCTGACGGCGGGTAATTTTGTTGCCGACCTGATGCACCTTAATC‑GCTTCCATAATCTGGAAACCCAC‑GGGTTAGCACGGGTTAAGGCTGGTCATCGGGTCCCGGAAGATC‑A‑TCGCCACTTCGGCACCCACCAGGGTGCGGGGCTCTTTTTCTGAGAGACGCTGCAAATCCTGGCCGTTTCACTCCCGGTTTTCTGCCATTACGCGGCCCGGATAATCAATCAAcccccatattgcc                                                                        >  1:191339/1‑240 (MQ=255)
                                                                                                                                                           tCAATCGCTCGCTGACGGCGGGTAATTTTTTTGCAGACCTGGTGCACCTTACTC‑GCTTCCCTAATCTGGAAAACCAC‑GGGTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATC‑A‑TCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATAAGCTGCAAATCCTGGCCGTTAAAATCCAGTTTTTCTGCCATTACCCGGCCCGGGTAATCACTCAGCCCCCTACTCGCCCGTgg                                                                   >  1:144990/1‑250 (MQ=255)
                                                                                                                                                            cAATCGCTCGCTGACGGCGGGGACTTTTGTTGCCGCCCTGATTCACCTTAATC‑GCCTCCATAATCTGGAAAACCAC‑GGGTTTGCACGGGGTAAGGCTGGTCATCGGGTCCTGGAAGATC‑A‑TCGCCACTTCGGCACCCACCAGGATGCGGCGCTCTTTTTCTGAGATACGCCGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACACGGCCCGGGTAATAAATAAGCCCCATAATCGCCAgcga                                                                   >  1:208448/1‑247 (MQ=255)
                                                                                                                                                                                gTACTTTTGTTGCCGCCCTGATGCACCTTAATC‑GCTTCCATAATCTGGAAACCCAC‑GGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATC‑A‑TCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGcc                                              <  1:127068/251‑1 (MQ=255)
                                                                                                                                                                                     tttGTTGCCGCCCTGATGCACCTTAATC‑GCTTCCATAATCTGGAAACCCAC‑GGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATC‑A‑TCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCCGCCCGGATAATCAATCAGCCCCATCATCGCCAGTGAACTGACCGACTGACCGGAGCCGGAcc                                         >  2:61375/1‑250 (MQ=255)
                                                                                                                                                                                                     tGCACCTTAATC‑GCTTCCATAATCTGGAAACCCAC‑GGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATC‑A‑TCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGAc                         >  2:131870/1‑251 (MQ=255)
                                                                                                                                                                                                                           aTCTGGAAACCCAC‑GGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATC‑A‑TCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCt    >  1:100941/1‑251 (MQ=255)
                                                                                                                                                                                                                             cTGGAAACCCAC‑GGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATC‑A‑TCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGt  >  1:110288/1‑251 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             
CCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATC‑GCTTCCATAATCTGGAAACCCAC‑GGTGTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATC‑A‑TCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGT  >  NC_000913/3703296‑3703766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑