Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,696,157	G→T	P10H (CCT→CAT)	yhjR ←	PF10945 family protein YhjR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,696,157	0	G	T	100.0%	92.2 / NA	26	P10H (CCT→CAT)	yhjR	PF10945 family protein YhjR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/13);  total (13/13)

GGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCTTCTAAATTGATTCACATGCTAAATCTGATAAGTTTTAAT  >  NC_000913/3695926‑3696395                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               ggcggTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCCGTAAGGCCATTCACCAATCCCCTCCTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATAGCGGGATCATGCAGAGTATCTGGTTCAtt                                                                                                                                                                                                                             >  1:114853/1‑251 (MQ=255)    ggTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGtt                                                                                                                                                                                                                          >  2:133364/1‑251 (MQ=255)             tgttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACGCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGATGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCAttt                                                                                                                                                                                                                  <  2:157277/250‑1 (MQ=255)                    cccACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGAt                                                                                                                                                                                                          <  1:133364/251‑1 (MQ=255)                             ccccGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATg                                                                                                                                                                                                   <  1:224634/249‑1 (MQ=255)                                  cACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAac                                                                                                                                                                                            <  1:204438/251‑1 (MQ=255)                                   aCCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAacc                                                                                                                                                                                           <  2:188227/251‑1 (MQ=255)                                                              cACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCg                                                                                                                                                                <  1:85616/251‑1 (MQ=255)                                                                                gttgCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGttt                                                                                                                                              <  1:45300/251‑1 (MQ=255)                                                                                     gcAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGAAAATTCTAACCGTGAGCATTTCGCCGGTTTATTGt                                                                                                                                         >  2:37999/1‑251 (MQ=255)                                                                                                    aaCGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACggg                                                                                                                          >  2:202275/1‑251 (MQ=255)                                                                                                            ccGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTTCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCGTGATCCCTATGAAATTACCGTACCGTTCATGCGTCAATTCTAACCGTGAGCATTCCGCCTGTTCATTGTATACGCCgatactcgattttgat                                                                                                                  <  2:212124/250‑15 (MQ=255)                                                                                                                           ggCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTc                                                                                                   >  1:113899/1‑251 (MQ=255)                                                                                                                                   gTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACg                                                                                            <  1:202275/250‑1 (MQ=255)                                                                                                                                             ggAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCgg                                                                                                                                                 >  2:42212/1‑187 (MQ=255)                                                                                                                                             ggAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCgg                                                                                                                                                 <  1:42212/187‑1 (MQ=255)                                                                                                                                             ggAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGaaaa                                                                                  >  1:93172/1‑250 (MQ=255)                                                                                                                                                              cAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGAc                                                                <  1:189261/251‑1 (MQ=255)                                                                                                                                                                      ggCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATc                                                         >  1:139004/1‑250 (MQ=255)                                                                                                                                                                           tGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGt                                                   >  2:170327/1‑251 (MQ=255)                                                                                                                                                                                   ccTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGAc                                           <  2:164324/251‑1 (MQ=255)                                                                                                                                                                                      gCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCtt                                        >  2:74396/1‑251 (MQ=255)                                                                                                                                                                                            aCGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCTTCTAAAt                                  >  1:22795/1‑251 (MQ=255)                                                                                                                                                                                                              ggAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATATCCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCTTCTAAATTGATTCACATGCTaa                   <  1:28960/248‑1 (MQ=255)                                                                                                                                                                                                               gAAGATATAGCATATCGCGGGATCATGAAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCTTCTAAATTGATTCACATGCTAAATCt               >  1:62481/1‑251 (MQ=255)                                                                                                                                                                                                                            aTCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCTTCTAAATTGATTCACATGCTAAATCTGATAAGTTTTAAt  >  1:9529/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               GGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCTTCTAAATTGATTCACATGCTAAATCTGATAAGTTTTAAT  >  NC_000913/3695926‑3696395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑