Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,689,145	G→T	T10K (ACG→AAG)	bcsC ←	cellulose synthase outer membrane channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,689,145	0	G	T	100.0%	107.7 / NA	31	T10K (ACG→AAG)	bcsC	cellulose synthase outer membrane channel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/17);  total (14/17)

TAACTGCGACAGCCGATCGAGCTGTTTTTGCGCGCCATCAATATCGCCCTGACGTAACAAAGAACGGAAACGGGCGGCAACGACGTCCGGGTTATTCGGATCAATAAGTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTAAAAAGGGCAGCATGGCGGCAGAAAAACCGACTGGTCCTTTA  >  NC_000913/3688901‑3689380                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            tAACTGCGACAGCCGATCGAGCTGTTTTTGCGCGCCATCAATATCGCCCTGACGTAACAAAGAACGGAAACGGGCGGCAACGACGTCCGGGTTATTCGGATCAATAAGTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAat                                                                                                                                                                                                                                        >  2:69484/1‑250 (MQ=255)  aacggCGACAGCCGATCGAGCTGTTTTTGCGCGCCATCAATATCGCCCTGACGTAACAAAGAACGGAAACGGGCGGCAACGCCGTCCGGGGTATTCGGATCAATAAGTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGAACATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAata                                                                                                                                                                                                                                       <  1:220366/246‑1 (MQ=255)      gCGACAGCCGATCGAGCTGTTTTTGCGCGCCATCAATATCGCCCTGACGTAACAAAGAACGGAAACGGGCGGCAACGACGTCCGGGTTATTCGGATCAATAAGTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGttt                                                                                                                                                                                                                                  <  1:54732/251‑1 (MQ=255)           aGCCGATCGAGCTGTTTTTGCGCGCCATCAATATCGCCCTGACGTAACAAAGAACGGAAACGGGCGGCAACGACGTCCGGGTTATTCGGATCAATAAGTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAgtgt                                                                                                                                                                                                                             >  1:209200/1‑251 (MQ=255)                  cGAGCTGTTTTTGCGCGCCATCAATATCGCCCTGACGTAACAAAGAACGGAAACGGGCGGCAACGACGTCCGGGTTATTCGGATCAATAAGTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTg                                                                                                                                                                                                                      >  1:225952/1‑251 (MQ=255)                   gAGCTGTTTTTGCGCGCCATCAATATCGCCCTGACGTAACAAAGAACGGAAACGGGCGGCAACGACGTCCGGGTTATTCGGATCAATAATTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTgc                                                                                                                                                                                                                     <  2:110783/251‑1 (MQ=255)                         tttttGCGCGCCATCAATATCGCCCTGACGTAACAAAGAACGGAAACGGGCGGCAACGACGTCCGGGTTATTCGGATCAATAAGTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTc                                                                                                                                                                                                               >  2:69745/1‑251 (MQ=255)                               cgcgcCATCAATATCGCCCTGACGTAACAAAGAACGGAAACGGGCGGCAACGACGTCCGGGTTATTCGGATCAATAAGTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGcc                                                                                                                                                                                                         >  2:119125/1‑251 (MQ=255)                                                  tGACGTAACAAAGAACGGAAACGGGCGGCAACGACGTCCGGGTTATTCGGATCAATAAGTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTcc                                                                                                                                                                                      >  1:177039/1‑250 (MQ=255)                                                                       cGGGCGGCAACGACGTCCGGGTTATTCGGATCAATAAGTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTGATAACTCACCTTTTGTCGAGAAGCGGaaa                                                                                                                                                                 <  1:119125/251‑1 (MQ=255)                                                                              cAACGACGTCCGGGTTATTCGGATCAATAAGTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGtt                                                                                                                                                          <  1:77898/251‑1 (MQ=255)                                                                              cAACGACGTCCGGGGTATTCGGATCAATAAGTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGtt                                                                                                                                                          >  2:201470/1‑251 (MQ=255)                                                                                 ggacgTCCGGGTTATTCGAATCAATAAGTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAt                                                                                                                                                       <  2:226263/250‑1 (MQ=255)                                                                                    cgTCCGGGTTATTCGGATCAATAAGTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATccc                                                                                                                                                    <  1:164211/251‑1 (MQ=255)                                                                                    cgTCCGGGTTATTCGGATCAATAAGTTCCAGCAGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATccc                                                                                                                                                    >  2:137213/1‑251 (MQ=255)                                                                                                 cGGATCAATAAGTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCaaa                                                                                                                                       <  1:65217/251‑1 (MQ=255)                                                                                                                  gCCGATATAACGACTGTTGCACCAGATCTGCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGAACATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGt                                                                                                                      <  1:99435/251‑1 (MQ=255)                                                                                                                                         aGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGa                                                                                               <  2:209200/251‑1 (MQ=255)                                                                                                                                               tCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCTAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGaaa                                                                                             <  1:19930/247‑1 (MQ=255)                                                                                                                                                  cGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCg                                                                                      <  1:135711/251‑1 (MQ=255)                                                                                                                                                              tCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACgcgctg                                                                           <  2:210863/250‑1 (MQ=255)                                                                                                                                                                                 ccAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCAt                                                       <  1:152979/251‑1 (MQ=255)                                                                                                                                                                                              cTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTa                                           >  1:184508/1‑250 (MQ=255)                                                                                                                                                                                                   cGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTAAAAAgg                                     <  1:105068/251‑1 (MQ=255)                                                                                                                                                                                                    ggTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTAAAAAggg                                    >  1:172031/1‑251 (MQ=255)                                                                                                                                                                                                    ggTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTAAAAAggg                                    >  1:158818/1‑251 (MQ=255)                                                                                                                                                                                                                      cATCGGCATGACGGCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTAAAAAGGGCAGCATGGCGGCAGaaa                   >  2:152130/1‑250 (MQ=255)                                                                                                                                                                                                                              tgaaggCCAGACAGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCACAGTAAGGTAATAAATCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTAAAAAGGGCAGCATGGCGGCAGAAAAACCGCCTg          >  2:106881/5‑251 (MQ=255)                                                                                                                                                                                                                                  ggCCAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCTCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCAGCGCATCGCGGTTTTGTAAAAAGGGCAGCATGGCGGCAGAAAAACCGACTGGTcc      <  2:40368/251‑1 (MQ=255)                                                                                                                                                                                                                                     cAGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTAAAAAGGGCAGCATGGCGGCAGAAAAACCGACTGGTCCttt   >  1:112615/1‑251 (MQ=255)                                                                                                                                                                                                                                      aGACCGAGGGAAAGCTTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTAAAAAGGGCAGCATGGCGGCAGAAAAACCGACTTGTCCTTTa  <  1:171964/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            TAACTGCGACAGCCGATCGAGCTGTTTTTGCGCGCCATCAATATCGCCCTGACGTAACAAAGAACGGAAACGGGCGGCAACGACGTCCGGGTTATTCGGATCAATAAGTTCCAGCCGATATAACGACTGTTGCACCAGATCTTCACGATGGGTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTAAAAAGGGCAGCATGGCGGCAGAAAAACCGACTGGTCCTTTA  >  NC_000913/3688901‑3689380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑