Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,329,743	G→T	D261Y (GAT→TAT)	dacB →	peptidoglycan DD‑endopeptidase DacB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,329,743	0	G	T	100.0%	101.4 / NA	28	D261Y (GAT→TAT)	dacB	peptidoglycan DD‑endopeptidase DacB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/16);  total (12/16)

GATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACA‑TGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCT  >  NC_000913/3329518‑3329981                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 gATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCa                                                                                                                                                                                                                          <  2:186841/250‑1 (MQ=255)    atGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATGCTGAAATATGAGTTAAAACAGGCGGGTATCACCt                                                                                                                                                                                                                       <  2:75124/250‑1 (MQ=255)                  cgTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTCTGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGAATCACCTGGAGCGGAACAc‑tgc                                                                                                                                                                                                       >  2:18991/1‑251 (MQ=255)                            tattaCCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGGACAC‑TGTTGCGCCAGAc                                                                                                                                                                                             >  2:50974/1‑251 (MQ=255)                                     gTTACGATGTTCAGCCAGGTACGCACCCGCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCAAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTaa                                                                                                                                                                                    <  2:56739/251‑1 (MQ=255)                                                 aGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCTGGAAc                                                                                                                                                                        <  1:11107/251‑1 (MQ=255)                                                             ccccTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGGGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAg                                                                                                                                                            <  1:131784/250‑1 (MQ=255)                                                             aCCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGGACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCCGGAACGGCAGTTGcc                                                                                                                                                              >  2:74675/1‑249 (MQ=255)                                                                  cccccGTGGTTCTGCAGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAAAAGGCGGGATTCACCTGGAGCGGA‑CACCTGATGGGCCAAACTCAGGTTAACGAACCTGGAACGGGGGTTGCCAGCaaa                                                                                                                                                        >  2:23821/1‑250 (MQ=255)                                                                               gCCGAAGCGCAATACTGCGAACGGGATGTGGTGCCAGGCGACCGGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGTCCCCGCt                                                                                                                                          <  2:190144/251‑1 (MQ=255)                                                                                         aatccggCGAACGGGATGTGGGGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGGTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTg                                                                                                                                <  2:144815/245‑1 (MQ=255)                                                                                            aCTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCtt                                                                                                                             <  1:80339/251‑1 (MQ=255)                                                                                                cGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTa                                                                                                                                                                    <  1:122598/208‑1 (MQ=255)                                                                                                                      gACCTGAACCGCTTTACGCTGACGGGAGGCCTTCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGacaa                                                                                                   <  1:83773/251‑1 (MQ=255)                                                                                                                               cGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACA‑TGATCGc                                                                                         <  1:111597/251‑1 (MQ=255)                                                                                                                                            ggggATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACA‑TGATCGCCGATACGGTTTTc                                                                            <  2:141614/250‑1 (MQ=255)                                                                                                                                               gATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACA‑TGATCGCCGATACGGTTTTCCGc                                                                         <  1:124485/251‑1 (MQ=255)                                                                                                                                                      gccACAACTTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGGTAAAGAACCTGGAACGGGAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAATCGGACAACA‑TGATCGCCGATACGGGTTTCCGCATGAt                                                                    >  1:9867/1‑249 (MQ=255)                                                                                                                                                              gTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGAAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGAGGAACAC‑TGCTGCGCCCGACTCCGGGTAACGAACCTGGCACGGTAGTTGCCCGTAAACCGTCGGACCCCCTGCCAGATCCGCTTTAGATTATGCTGAAAAAGTCGGGCA‑CACTGCACGCCGATACGGGTTTTCGCATGATAAGACATgcg                                                          >  2:221760/1‑251 (MQ=255)                                                                                                                                                                      ccGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACA‑TGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCaa                                                  <  1:50974/251‑1 (MQ=255)                                                                                                                                                                                  gCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGGACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAAAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACA‑TGATCGCCGATACGGGTTTCCGCATGATAGGCCATGCGCGCCTCAATGTGCCTGGAAc                                      >  1:180474/1‑251 (MQ=255)                                                                                                                                                                                           gTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGGTAACGAACCTGGAACGGTAGTTGCCAGTAAAAAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACA‑TGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGGGCTTCAATGTGCCTGGAACATGGCgggg                             >  1:41903/1‑250 (MQ=255)                                                                                                                                                                                             gCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACA‑TGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGcc                            <  2:199569/250‑1 (MQ=255)                                                                                                                                                                                                       gCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCGGCACGATCTGCTTAAGATTATGCt                                                                                                                  <  1:78994/155‑1 (MQ=255)                                                                                                                                                                                                         cAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAAACTGGAACGGGAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACA‑TGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGGCGGGTCGGGCGCCg               >  1:67055/1‑251 (MQ=255)                                                                                                                                                                                                             tATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGGTAACGAACCTGGAACGGGAGGTGGCAGTAAACAGTCGGCCCCGCTGCACGAACTGCTTTAGATTATGCTGAAAAAGTCGGACAACA‑TGATCGCCGATACGGTTTTTCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACCTGgcggggcggggtgggcgccggggg           >  2:107525/1‑237 (MQ=255)                                                                                                                                                                                                                      gCAATTCTGAAATATGAGTTAAAACAGGCGGGTAACACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAAACTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCCCGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAAAA‑TGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGGCGGACGCCGTGCGGCAGATCCt  >  1:20941/1‑251 (MQ=255)                                                                                                                                                                                                                      gCAATTCTGAAATATGAGTTAAAACAGGCGGGAATCACCTGGAGCGGCACAC‑TGCTGCGCCCGACTCAGGGTAACGAACCTGGAACGGTCGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACA‑TGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGGCGGGTCGGGCGCCGGGCGGCAGaaccc  >  1:194383/1‑247 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 GATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACAC‑TGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACA‑TGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCT  >  NC_000913/3329518‑3329981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑