Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,142,911	C→T	V27I (GTC→ATC)	hybC ←	hydrogenase 2 large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,142,911	0	C	T	100.0%	54.5 / NA	17	V27I (GTC→ATC)	hybC	hydrogenase 2 large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/11);  total (6/11)

AGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATTCTCTGGCTCATGCTTTGCTCGCCTCATGACGATTATGATCGTTTTGTTTTAAAGGAGGAAGTATCGGCAGTAGACGAATGAGTACGATGTAAGCGCAAATCTCAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAACCGCC  >  NC_000913/3142665‑3143144                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          aGAAAGGAACAATATGTTCATGCGTGGTGTGCGAAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGTAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCCTGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGATGCCa                                                                                                                                                                                                                                       <  2:121922/251‑1 (MQ=255)                ggTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGATGCCATTTTCGATTTCGCa                                                                                                                                                                                                                         >  1:85185/1‑250 (MQ=255)                                                   cGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGATGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCgg                                                                                                                                                                                     >  1:2104/1‑251 (MQ=255)                                                                                       aGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGATGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATTCTCTGGCTCATGCTTTGCTCGcc                                                                                                                                                 >  2:73462/1‑251 (MQ=255)                                                                                               ttCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGATGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATTCTCTGGCTCATGCTTTGCTCGCCTCATGAc                                                                                                                                          <  1:143030/250‑1 (MQ=255)                                                                                                tCTGCCGCACGAACGTAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGATGCCATTTTCGAGTTCGCAATCGATGCGTAAATGCCCCTCAATACGGTTTACCGGATCAATAGTAATTCTCTGGGGCATGCTTTGCTGGCCTCATGACg                                                                                                                                         <  2:28753/250‑1 (MQ=255)                                                                                                   gCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGATGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATTCTCTGGCTCATGCTTTGCTCGCCTCATGACGAtt                                                                                                                                      <  2:131489/250‑1 (MQ=255)                                                                                                   gCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGATGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATTCTCTGGCTCATGCTTTGCTCGCCTCATGACGATTa                                                                                                                                     <  1:129/251‑1 (MQ=255)                                                                                                      gCACGAACGGAAGACAGCGCGGGAGTGGTATTACATACGCCACAGATGCGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTGCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGATGCCTTTTTCTATTTCGCAATCGATGGGTGAATGCCCCTCAAGAGCGGTTGCCGTATCAAGAGTTTTTCTCTTGTTCATGGTTTGCGCGCTTCATGAGGATTATGa                                                                                                                                  <  2:116441/251‑1 (MQ=255)                                                                                                             gggAAGACAGTGCGTGAGTTGTATTACATACGCCACAGAGACGTTGCACAATCATCCAGGCAGCGCGCGGAGCGCGGTGTTTCACGATCTCTTCCATGCCGCGCCACGTGGTGCCGGAGGCCCAGGCTGTCGAAACGATACCATTTTCGAGTGCGCAAGCGAGGCGGAAATTCCCCCCTATACGGGTTACCGCATCGATAGTAATTCTGTGGGTTATGCGATGCTCGCCTCATGACGATTATGATCgtttt                                                                                                                           <  1:140538/250‑1 (MQ=255)                                                                                                                                                      cGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGATGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATTCTCTGGCTCATGCTTTGCTCGCCTCATGACGATTATGATCGTTTTGTTTTAAAGGAGGAAGTATCGGCAGTAGACGAATGAGTACg                                                                                  <  2:65155/251‑1 (MQ=255)                                                                                                                                                                        gCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGATGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATTCTCTTGCTCATGCGTTGCTCGCCTCa                                                                                                                                              <  1:54326/173‑1 (MQ=255)                                                                                                                                                                        gCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGATGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATTCTCTGGCTCATGCTTTGCTCGCCTCa                                                                                                                                              >  2:54326/1‑173 (MQ=255)                                                                                                                                                                                                                          ggTACCGGAAGCCCATGCTTTCGAAACGATGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACTGGTTACCGGATCAATAGTAATGCTCTGGCTCATGCTGTGCTCGCCTCATGACGATTATGATCGTTTTGTTTTAAAGGAGGAAGTATCGGCAGGAGACGAATGAGTACGATGTAAGCGCAAAGCTCGATAGCCACAATACCAAGAGAAATCAACAGTTCTTCCCAGTTCGGGAAGt               <  2:92916/250‑1 (MQ=255)                                                                                                                                                                                                                                ggAAGCCCATGCTTTCGAAACGATGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATTCTCTGGCTCATGCTTTGCTCGCCTCATGACGATTATGATCGTTTTGTTTTAAAGGAGGAAGTATCGGCAGTAGACGAATGAGTACGATGTAAGCGCAAATCTCAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTa        <  1:82427/251‑1 (MQ=255)                                                                                                                                                                                                                                 gAAGCCCATGCTTTCGAAACGATGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATTCTCTGGCTCATGCTTTGCTCGCCTCATGACGATTATGATCGTTTTGTTTTAAAGGAGGAAGTATCGGCAGTAGACGAATGAGTACGATGTAAGCGCAAATCTCAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTa        >  2:182375/1‑250 (MQ=255)                                                                                                                                                                                                                                      ccATGCTTTCGAAACGATGCCAATTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATTCTCTGGCTCATGCTTTGCTCGCCTCATGACGATTATGATCGTTTTGTTTTAAAGGAGGAAGTATCGGCAGTAGACGAATGAGTACGATGTAAGCGCAAATCTCAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAAccgcc  >  1:198091/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          AGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATTCTCTGGCTCATGCTTTGCTCGCCTCATGACGATTATGATCGTTTTGTTTTAAAGGAGGAAGTATCGGCAGTAGACGAATGAGTACGATGTAAGCGCAAATCTCAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAACCGCC  >  NC_000913/3142665‑3143144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑