Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,612,191	G→T	G98* (GGA→TGA)	hyfR →	DNA‑binding transcriptional activator HyfR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,612,191	0	G	T	100.0%	55.2 / NA	18	G98* (GGA→TGA)	hyfR	DNA‑binding transcriptional activator HyfR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/7);  total (11/7)

ACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATC  >  NC_000913/2611961‑2612421                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       aCGGACTGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGTAGCTACGCttt                                                                                                                                                                                                                    <  2:169455/251‑1 (MQ=255)                         cacaGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGtttt                                                                                                                                                                                            >  1:103069/1‑251 (MQ=255)                                               tttATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGATTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCCGAGCTACGCTTTATGCGCAcc                                                                                                                                                                                                           <  2:217401/214‑1 (MQ=255)                                               tttATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTAAGCCCGGTCCAAATTATTGAACTGCATGTTCTCAACAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGAATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCAcc                                                                                                                                                                                                           >  1:217401/1‑214 (MQ=255)                                                          ccTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGAAGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAActggctg                                                                                                                                                           >  1:50334/1‑251 (MQ=255)                                                                               cgTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGtt                                                                                                                                      >  2:200067/1‑251 (MQ=255)                                                                                                     tGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAatgatg                                                                                                                >  2:130123/1‑251 (MQ=255)                                                                                                                    cAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTTACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACGGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGCAGAACAGCAGGt                                                                                                   <  1:11721/249‑1 (MQ=255)                                                                                                                                      ccGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCTTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCGCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCGGgaga                                                                               <  2:94600/251‑1 (MQ=255)                                                                                                                                             cATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGACTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGaa                                                                              >  2:65679/1‑245 (MQ=255)                                                                                                                                                   gatgGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATGATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTGTAACTGGCTGGCGCGGATAATCACCCCGGTTCTTGAATCATGGCTCAATGATGAAGAACAGCAGGGGGCGGGGCGTTTTCTGGAGAAAGATCGCGatc                                                                   <  2:145530/250‑1 (MQ=255)                                                                                                                                                                  aCGGCAACAAAAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCATTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGt                                                   >  1:88699/1‑251 (MQ=255)                                                                                                                                                                        acaacaGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGatat                                             <  2:59769/251‑1 (MQ=255)                                                                                                                                                                                      gggCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGAACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGAGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGAACTGGTTGATATTACTAATGCAGTGc                               >  1:100852/1‑251 (MQ=255)                                                                                                                                                                                         cGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGt                            <  2:55198/251‑1 (MQ=255)                                                                                                                                                                                                    attattCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGAt                 >  2:128114/1‑251 (MQ=255)                                                                                                                                                                                                       attCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTAAGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGAtctc              >  1:145713/1‑251 (MQ=255)                                                                                                                                                                                                                   ccGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCACACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGGACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGAtt  >  2:147562/1‑250 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       ACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATC  >  NC_000913/2611961‑2612421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑