Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,599,947	G→T	L14F (TTG→TTT)	gcvR →	putative transcriptional regulator GcvR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,599,947	0	G	T	100.0%	77.3 / NA	22	L14F (TTG→TTT)	gcvR	putative transcriptional regulator GcvR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/12);  total (10/12)

CGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAGC  >  NC_000913/2599737‑2600152                                                                                                                                                                                                                   |                                                                                                                                                                                                              cgcgACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACACGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTc                                                                                                                                                                       >  1:150309/1‑251 (MQ=255)          aCTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCagta                                                                                                                                                              <  2:168325/251‑1 (MQ=255)                                     gtgCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACa                                                                                                                                      <  2:72936/248‑1 (MQ=255)                                                      aatggaacgtttcgtctcgaaTTACAAGCAAGAAGACAGACCCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGACCAACTCACCCCTTCAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACACGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGAAAGTCGCCTGGCGATGCTgggg                                                                                                                  >  2:115932/21‑250 (MQ=255)                                                            aCGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGagaaga                                                                                                             <  1:41004/250‑1 (MQ=255)                                                                     aTGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGttt                                                                                                   <  2:3851/251‑1 (MQ=255)                                                                        gCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGGGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTttatt                                                                                                <  1:25460/251‑1 (MQ=255)                                                                           ttAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGATTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTAtg                                                                                             >  2:71737/1‑251 (MQ=255)                                                                                                      gATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCAtt                                                                  <  1:62135/251‑1 (MQ=255)                                                                                                             gtATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTg                                                            >  1:27854/1‑250 (MQ=255)                                                                                                                tGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGCCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTg                                                        >  1:205635/1‑251 (MQ=255)                                                                                                                          ttttATGCTTTCCTTAAGAACAACTCACCC‑TTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGtt                                              <  1:75267/250‑1 (MQ=255)                                                                                                                            ttATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTa                                             <  1:224307/250‑1 (MQ=255)                                                                                                                            ttATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTa                                             <  1:187154/250‑1 (MQ=255)                                                                                                                                        tAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGTAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTg                                >  1:124575/1‑251 (MQ=255)                                                                                                                                        tAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAgg                                  <  1:88988/249‑1 (MQ=255)                                                                                                                                        tAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTg                                >  1:65944/1‑251 (MQ=255)                                                                                                                                                 cTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGCATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTgg                       >  1:45061/1‑251 (MQ=255)                                                                                                                                                 cTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTgg                       >  1:65141/1‑251 (MQ=255)                                                                                                                                                          tAAAGGAATAACCAGATTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTa               <  1:71737/250‑1 (MQ=255)                                                                                                                                                            aaGGAATAACCAGTTTGACACGGTCATCGCAACATTATCTGGTGAGCACTGCGGTTGGTGCCGATCGCCCTGGAATGGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTGACCGTTGAAAGGTGCCGAACTGGATCTTTTAATc            <  2:72636/251‑1 (MQ=255)                                                                                                                                                                      cAGTTTGACACTGTCATCGCAACATTATCTGGCGATCACTGCGTTTGCTGCCGATCGACCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGACGAGTTCACGTTTATTATGCTGCTTTACGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTCCCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAgc  >  1:16371/1‑251 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                                                              CGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAGC  >  NC_000913/2599737‑2600152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑