BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,829,887	G→A	A293A (GCC→GCT)	astA ←	arginine N‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,829,887	0	G	A	100.0%	74.6 / NA	24	A293A (GCC→GCT)	astA	arginine N‑succinyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/10); total (14/10)

ATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTAT  >  NC_000913/1829650‑1830128

aTAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTCAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGGGATAATTTTCATTAGCGAAcaggcagg                                                                                                                                                                                                                                       >  1:29711/1‑251 (MQ=255)

                                      gATGCGCCCTGGCCCGTTATCCAGTCACCGTTCATCCATAAAGCCATACCGTTCTCTCCTCTGCGCACAGGCACACCAGACCACAGCGACCCCCGACCTGCCATTTGAGGGCATCCACTTGTGCGGCGGTTCCAATCAACAGCCCGGTTTCCGGATCCGTACGCCACAAAACCACTCGGAAATGGGGCTAATTTTCATTAGCGACCAGGCAGGCTGGGAAATGCCCCTGTGCAAGCTGCCC‑CTCTGcccct                                                                                                                                                                                                >  1:17957/1‑248 (MQ=255)

                                       aTGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCCAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCCCGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACtt                                                                                                                                                                                               >  1:136336/1‑251 (MQ=255)

                                                    cGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGAcc                                                                                                                                                                                  >  2:65104/1‑250 (MQ=255)

                                                        aTCCAGTCCCCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGGTGCCGGATCGGTACGCACCAGCCCCACGCGGAAATGGGGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAAACGCCCTGCGCAGGCTGCCCCTTC‑GCCACTTTCACCCGCCGCCttttt                                                                                                                                                                              >  2:192847/1‑250 (MQ=255)

                                                                                  cATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAGCGCTAGCCCCGGCGTGGCATTTGAGGGCATCCATTTGTGCTGCTGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCTGCACCACGCGGAAATGGTGATAAGTTTCATTAGCGACCAGGCAGGCAGGGAAATCGTCCTTCGCATGCTGCCC‑ATGTGCCACTTCCCCCGGCCGGCTTTTTCGGATGATGCTCACGCGGtataggtc                                                                                                                                                    <  2:149875/251‑8 (MQ=255)

                                                                                          tttCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCa                                                                                                                                             >  1:203930/1‑250 (MQ=255)

                                                                                                                   aGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATg                                                                                                                     <  1:103821/249‑1 (MQ=255)

                                                                                                                   aGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGGCGATGTCCCCCTCAAGCGTCGGCCCCCCGTCCAAGATg                                                                                                                     >  2:103821/1‑249 (MQ=255)

                                                                                                                            cgATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGGCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGtt                                                                                                          <  2:191785/251‑1 (MQ=255)

                                                                                                                                         ggCATTTGAGGGCATCCAGTTGTGCGGCGGTTTAAATCAAACGCCCGGTTGCCGGGTCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATAGCCCTGCGCAGGCTGACC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGaaa                                                                                              >  1:13787/1‑250 (MQ=255)

                                                                                                                                              ttGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGACGGATCGGCACGAAACAGCACCACGCGGAAATGGGGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCCGCCGACTTTTACCGGTGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACcttt                                                                                        >  1:148893/1‑250 (MQ=255)

                                                                                                                                                gAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGGCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACcttctt                                                                                      >  1:210699/1‑251 (MQ=255)

                                                                                                                                                          aGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCCCCCTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCCGCAc                                                                            >  1:205961/1‑251 (MQ=255)

                                                                                                                                                           gTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCAcc                                                                           <  1:20199/251‑1 (MQ=255)

                                                                                                                                                              gtgCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACcgc                                                                         <  1:166783/250‑1 (MQ=255)

                                                                                                                                                                        tAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGCCTTTTACGGATGGAGCGCACGCGGTCGATGGCACACTCAAGCGTCGGCACACCGTCAAAGATGTCGATATAGTTACGGAAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGgcg                                                              >  2:182576/1‑251 (MQ=255)

                                                                                                                                                                                aaCGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGtttac                                                      >  1:9968/1‑249 (MQ=255)

                                                                                                                                                                                      cGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATg                                                 <  1:65104/250‑1 (MQ=255)

                                                                                                                                                                                                   ggTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTGCCTGATCGATGa                                   <  1:200911/251‑1 (MQ=255)

                                                                                                                                                                                                         caccagcaccaCGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCt                             <  2:71892/251‑1 (MQ=255)

                                                                                                                                                                                                                  cacGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCt                    <  2:205961/251‑1 (MQ=255)

                                                                                                                                                                                                                             ggTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAg         <  2:136336/251‑1 (MQ=255)

                                                                                                                                                                                                                                       ttCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGtat  >  1:207067/1‑248 (MQ=255)

ATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCC‑TTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTAT  >  NC_000913/1829650‑1830128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`