Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	229,552	C→T	S129F (TCC→TTC)	dkgB →	methylglyoxal reductase DkgB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	229,552	0	C	T	100.0%	47.8 / NA	15	S129F (TCC→TTC)	dkgB	methylglyoxal reductase DkgB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/5);  total (10/5)

GTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTCCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGG  >  NC_000913/229326‑229797                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      gTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATgg                                                                                                                                                                                                                                >  2:59089/1‑250 (MQ=255)                     cATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTTCTGCTGTTg                                                                                                                                                                                                           <  1:169048/251‑1 (MQ=255)                                aaaTCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAAc                                                                                                                                                                                                >  1:31473/1‑251 (MQ=255)                                                   cAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCACGTTGATGGAAAAAGCGATTGCTGCTGTTGGGGCTGTAAACATCGCTACTAACCAGAttt                                                                                                                                                                             >  2:64002/1‑250 (MQ=255)                                                                 tGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTTACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTa                                                                                                                                                                <  1:143212/250‑1 (MQ=255)                                                                                    gagCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTgg                                                                                                                                            >  1:201503/1‑251 (MQ=255)                                                                                                 ttGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTa                                                                                                                                >  2:181127/1‑250 (MQ=255)                                                                                                      tACCGATTCTGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTACACAGc                                                                                                                          >  2:191780/1‑251 (MQ=255)                                                                                                              aTGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCAt                                                                                                                   <  1:180957/250‑1 (MQ=255)                                                                                                                                       gCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTgg                                                                                         >  2:61907/1‑251 (MQ=255)                                                                                                                                                                       agTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTAtt                                                         >  2:96055/1‑251 (MQ=255)                                                                                                                                                                            aTGCAGGCGCTGCTGGAATCCAAAAAACAAGGGCTGACGCGTGAGATCGGTAGGTTCAACTTCACGATCCCGTTGAGGGAAAAAGCGATTGCAGCTGTTGGTGCTGAAAACATCGCGACTAACCAGATGGAACTCTCTCCGTAACTGCAAAACCGTAAAGTGGTTGCCGGGGCTAAACAGGACGGCATCCTTAGTACGTCCTATATGACGGTTGCGTAGGGTAAGGCCCTGAAAGATGGGGTGAGTGCTc                                                     <  2:17645/250‑1 (MQ=255)                                                                                                                                                                                aGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATc                                                >  2:200642/1‑251 (MQ=255)                                                                                                                                                                                                          gggCTGACGCGTGAGATCGGTATTTTCAACTTCCCGATCCCGTTGATGGCAAAAGCGCTTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTATCCTTATCTGAAAAACCGTAAAGTGGTTGCATGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGc                      >  2:213122/1‑251 (MQ=255)                                                                                                                                                                                                                               aTTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGAGGCTGGCGTggg  <  2:151015/250‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      GTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTCCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGG  >  NC_000913/229326‑229797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑