Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	42,108	G→A	intergenic (‑177/‑295)	caiT ← / → fixA	L‑carnitine:gamma‑butyrobetaine antiporter/putative electron transfer flavoprotein FixA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	42,108	0	G	A	100.0%	29.0 / NA	11	intergenic (‑177/‑295)	caiT/fixA	L‑carnitine:gamma‑butyrobetaine antiporter/putative electron transfer flavoprotein FixA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/6);  total (5/6)

AAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAGGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTTATTTCAAAATTAAAGGGCGTGATATCTGTAAT  >  NC_000913/41888‑42353                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      aaaaCCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTaataat                                                                                                                                                                                                                         <  2:119968/251‑1 (MQ=255)          ggTTCTATTCCCGTTTTTCTCTTTTCATTCTTCAGGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCGAACTCATAATGGTTATGCAATAAGCTGTATTCTGTGATTGGTATCACATTTGGGTTGCGTGTGAATATAAGGCTTGGGTGCGTGAATTTTGATTAATAAGCAGgttgtt                                                                                                                                                                                                                <  2:14165/251‑1 (MQ=255)                                      tcttcATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCGCGTGGACGCGTTAATGGCTAACTCATAATGAGTATTCAATAATCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAATTAAcc                                                                                                                                                                                     <  2:88157/250‑2 (MQ=255)                                        ttcATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCt                                                                                                                                                                                  >  1:182223/1‑251 (MQ=255)                                                                                                        ttAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTAATAATGGGAATTCAATAAGATGTATTCTGTGATTGGAATCACATTTTTGTTTCGGGTGAATAGAAGTCGTTTTTTCGTTAATTTTGATTAATAATAAGTTTGTTATGCTCTGTTGTGAGTAAAACATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGAGTTTTc                                                                                                                  >  1:133998/1‑251 (MQ=255)                                                                                                                                     cGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATCAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTg                                                                                      <  1:52424/250‑1 (MQ=255)                                                                                                                                                                         cAATAAGCTGTACTCTGGAATTGGTAACACCTTTTTGTTTAGGGCGAATAGAAGGCGCTTTTCCGCTAATTTTGATTAATCATCCGTTTATTATGCTCTGTTGTGAGTACAAAATAACATCTGACTTTCACTCTTGGTGATCCATAAACCAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTCTCTGCGTGTGACATTTTCAATAtt                                                 >  2:214488/1‑251 (MQ=255)                                                                                                                                                                                 tGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGCGAATAGAAGGCGATTTTTAGTTAATTTTGATTAATCATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGAATTTCAATATTGGTGATCCCTAAAACAATATTAAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGACCTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTgctt                                          >  2:195434/1‑247 (MQ=255)                                                                                                                                                                                            aTTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTaaa                                                                                                                                                                                             >  1:78691/1‑92 (MQ=255)                                                                                                                                                                                            aTTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTaaa                                                                                                                                                                                             <  2:78691/92‑1 (MQ=255)                                                                                                                                                                                                                        aTAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTTATTTCAAAATTAAAGGGCGTGATATCTGTAAt  <  2:80137/251‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      AAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAGGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTTATTTCAAAATTAAAGGGCGTGATATCTGTAAT  >  NC_000913/41888‑42353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑