Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,838,007	0	A	C	28.0%	23.3 / 14.5	25	T266P (ACT→CCT)	ynjC	putative ABC transporter membrane subunit YnjC
Reads supporting (aligned to +/- strand):  ref base A (6/12);  new base C (2/5);  total (8/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.80e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTG  >  NC_000913/1837766‑1838251                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     cGTCGCGCCTTCGCTGGTAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGTCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCACTATTcccc                                                                                                                                                                                                                                             <  2:119835/251‑1 (MQ=255) cGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCCCTATTcccc                                                                                                                                                                                                                                             >  1:72610/1‑251 (MQ=255) cGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCACTATTcccc                                                                                                                                                                                                                                             <  2:165005/251‑1 (MQ=255)           gcgcTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCACTATTCCCCGCGTAGAt                                                                                                                                                                                                                                     <  1:74125/249‑1 (MQ=255)                          aTGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCCCTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGc                                                                                                                                                                                                                    >  1:133087/1‑251 (MQ=255)                                gCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGcc                                                                                                                                                                                                              <  1:181320/251‑1 (MQ=255)                                                cGCTGTCGTGCGTGGATGTGCCATTTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAAt                                                                                                                                                                                              <  2:130678/251‑1 (MQ=255)                                                cGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCGGCTATGGCGCAGCTGGCGGCGCACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAAt                                                                                                                                                                                              <  1:102488/251‑1 (MQ=255)                                                           gTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCCCTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGAt                                                                                                                                                                                   <  1:41701/251‑1 (MQ=255)                                                                     aaaTTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTAccc                                                                                                                                                                         <  1:204251/250‑1 (MQ=255)                                                                          attCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGTTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGT‑TGATGCTGTTACTCGCCGCCTTCGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTaa                                                                                                                                                                     <  1:201976/250‑1 (MQ=255)                                                                                       gTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGGGCtgggg                                                                                                                                                       >  2:198270/1‑248 (MQ=255)                                                                                              cccGGCGCTGGGGGTAATTAGCTGTCAGTGGTTAACCCAGGCCGACATTGACCAACAAACAAAAGGCGCTCTTGCCTGCCTGCTGT‑TGATGCTGTTTCTCGCCGCCTCCGTTTTGCGGAGCTATCTGCTATGGCGCAGCGGGCGGCGCACTATTCCCCGTGTAGATGGCGTTGGCGTGCCTTCCACGCCTTTATTGCCGGGCAAGACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTc                                                                                                                                                <  1:36415/251‑1 (MQ=255)                                                                                                                 agtgggtaGTGGTAAACCCAGGGCAGCATTGCCCAACAAACAAAAGGCGTGTTTGACGGTCTGC‑GTGTGGTGCTTTTACGTTCCGCCTACGTTTTGCGGTGGTATCTGCATTGGTGCAGCGGGCGGCGCATTAGTCCCCGCGTAGATGGCGTTGTCAAGCCGGCCACGCCTTGATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGTTGTGCTGTGTGTGGTTCTGCTGTCGATTCTTGCGGa                                                                                                                             <  2:218836/244‑1 (MQ=255)                                                                                                                        aGTGTTTAACCCGGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCGTT‑TGATGCTGTTACTCGCGGCCTACGTTTTGCTGAGCTATCTGCTATGGCGAAGTGGGCGGCGCCCTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTcg                                                                                                                      <  2:127561/251‑1 (MQ=255)                                                                                                                                                acaaaaaaacaaaGGGCGCGCTTGCCAGCCTGCTGT‑TGATGCTGTTACTCTCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCACTATTCCCCGCGTAGATGTCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCtcatc                                                                                              <  2:183695/245‑1 (MQ=255)                                                                                                                                                                         cAGCCTGCTGT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCCCTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCtggtgg                                                                     <  1:218632/251‑1 (MQ=255)                                                                                                                                                                                ctgT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCt                                                                          >  1:43714/1‑239 (MQ=255)                                                                                                                                                                                ctgT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCt                                                                          <  2:43714/239‑1 (MQ=255)                                                                                                                                                                                  gT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCCCTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCa                                                            <  1:174673/251‑1 (MQ=255)                                                                                                                                                                                                         tACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTActgct                                      >  2:85025/1‑251 (MQ=255)                                                                                                                                                                                                           cGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCtgt                                    >  1:147899/1‑251 (MQ=255)                                                                                                                                                                                                                                       aGCTGGTGGCCCACTATCCCCCGAGTAGATGGCGCTCGCAAGCCTGCCAAAAACTCACTGCCAGGCAATACGCTGGCGATTTTTTTACCCCCAATCGGCCTGCTGTGTGTGGTTGTGAAAGCGATCCTCACGGATCAGTCGACGATCAATAGTGAAGCGCTAATCACAAACCTGAAACTGGGGCTGGAGGAGACATTCATTGCTTTGCTCCTGTTACTGCTGTGGCAGGAAAGGGGGccctactgtggccc        >  2:202956/1‑239 (MQ=255)                                                                                                                                                                                                                                            gcggcgCCCTATTCCCCGCGTAGGTGGGGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGGCACAGCGTCGCCGGTtgt   <  2:208678/251‑1 (MQ=255)                                                                                                                                                                                                                                             cggcgCACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTtgtg  >  2:125771/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     CGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGT‑TGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTATCTGCTATGGCGCAGCTGGCGGCGCACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTG  >  NC_000913/1837766‑1838251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑