Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,375,828	IS186 (+) +4 bp	coding (132‑135/963 nt)	menC ←	o‑succinylbenzoate synthase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2375828 =	0 (0.000)	16 (0.750)	15/482	0.5	100%	coding (135/963 nt)	menC	o‑succinylbenzoate synthase
	?	NC_000913	= 2515617	NA (NA)					noncoding (1345/1345 nt)	IS186	repeat region
*	?	NC_000913	= 2375831	0 (0.000)	20 (0.940)	18/482	0.3	100%	coding (132/963 nt)	menC	o‑succinylbenzoate synthase
	?	NC_000913	2514273 =	NA (NA)					noncoding (1/1345 nt)	IS186	repeat region

CCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGCCATTACACAGCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCAG  >  NC_000913/2375586‑2376075                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        ccTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGCCATTACACAGCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGAGCtccca                                                                                                                                                                                                                                                 <  2:148267‑M1/251‑6 (MQ=255)      cATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGCCATTACACAGCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCgcccataagc                                                                                                                                                                                                                                            <  2:69637‑M1/251‑11 (MQ=255)            atccagccatcgctggatttcccccccagaagtgccggatccgaaaagaagaactaactcgttgtggagaataacaaaaatggtcatctggagcttacaggtggccattcgtgggacagtatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggaGATCTCCCCCCACCCTTcgc                                                                                                                                                                                                                                      >  2:87123‑M1/232‑251 (MQ=255)               cagccatcgctggatttcccccccagaagtgccggatccgaaaagaagaactaactcgttgtggagaataacaaaaatggtcatctggagcttacaggtggccattcgtgggacagtatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggaGATCTCCCCCCACCCTTCGCGCt                                                                                                                                                                                                                                   <  1:123745‑M1/23‑1 (MQ=255)                 gccatcgctggatttcccccccagaagtgccggatccgaaaagaagaactaactcgttgtggagaataacaaaaatggtcatctggagcttacaggtggccattcgtgggacagtatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggaGATCTCCCCCCACCCTTCGCGCTCg                                                                                                                                                                                                                                 >  2:149083‑M1/227‑251 (MQ=255)                 gccatcgctggattgcccccccagaagtgccggatccgaaaagaagaactaactcgttgtggagaataacaaaaatggtcatctggagcttacaggtggccattcgtgggacagtatccctgacagcctacaaaacgcaattgaagaactcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggaGATCTCCCCCCACCCTTCGCGCTCg                                                                                                                                                                                                                                 >  1:112892‑M1/227‑251 (MQ=255)                 gTTTGAGGATCAGATCGTCCGGATCGCCATTACACAGCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCtcccataagcgctaacttaag                                                                                                                                                                                                                                 <  1:129487‑M1/251‑22 (MQ=255)                              aTCGTCCGGATCGCCATTACACAGCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCtcccataagcgctaacttaagggttgtggtatt                                                                                                                                                                                                                     >  1:69970‑M1/1‑217 (MQ=255)                                cGTCCGGATCGCCATTACACAGCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCtcccataagcgctaacttaagggttgtggtattacg                                                                                                                                                                                                                  <  2:198305‑M1/251‑37 (MQ=255)                                cGTCCGGATCGCCATTACACAGCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCtcccataagcgctaacttaagggttggggtattacg                                                                                                                                                                                                                  <  2:219310‑M1/251‑37 (MQ=255)                                  cccccagaagtgccggatccgaaaagaagaactaactcgttgtggagaataacaaaaatggtcatctggagcttacaggtggccagtcgtgggacagtatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggaGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAAc                                                                                                                                                                                                                >  2:131424‑M1/210‑251 (MQ=255)                                       agaagtgccggatccgaaaagaagaactaactcgttgtggagaataacaaaaatggtcatctggagcttacaggtggccattcgtgggacagtatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggaGATCTCCCCCCACCCTTCGCGCTCGCCGTCACGCAGGCAAACATACa                                                                                                                                                                                                           >  2:96441‑M1/205‑251 (MQ=255)                                                  atccgaaaagaagaactaactcgttgtggagaataacaaaaatggtcatctggagcttacaggtggccattcgtgggacagtatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggaGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCgg                                                                                                                                                                                                <  2:227072‑M1/58‑1 (MQ=255)                                                                actaactcgttgtggagaataacaaaaatggtcatctggagcttacaggtggccattcgtgggacagtatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggaGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCt                                                                                                                                                                                  <  1:192513‑M1/72‑1 (MQ=255)                                                                                   aaaaaaaaatggtcatctggagctgaaaggtggccattcgggggacagtatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtctaaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggaGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAAAATACACCCCGTCGCGGTTTTTTAACCGCCTGTCGCGCAGAACCAccccc                                                                                                                                                               >  2:51409‑M1/161‑251 (MQ=255)                                                                                              ggcagTGCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCACGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCGTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCtcccataagcgcttactgaagggtggtggtattacgcctgatatgatttaactggccgatgaattactcgcacgatagctgatcagcatttctg                                                                                                                                                        <  2:19542‑M1/245‑95 (MQ=255)                                                                                                              aggtggccattcgtgggacagtatccctgacagcctacaaaacgcaattgaagaacgcgaggcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggaGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGCCagcgggat                                                                                                                                    <  2:126234‑M1/118‑4 (MQ=255)                                                                                                                 aGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaaga                                                                                                                                 <  2:56221‑M1/251‑118 (MQ=255)                                                                                                                              gacagtatccctgacagactacaaaacgcaattcaagaaagcgaggcatcgtcttaacgaggcaacgacgcgtagcattcttccgatggttcaacccttaagttagcgcttatgggaGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGACCGTCGCGGGCTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGAACTGCCCGCGGTATACCTGCGCGCTACGc                                                                                                                     <  1:131146‑M1/133‑1 (MQ=255)                                                                                                                                     gAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgt                                                                                                              >  2:24218‑M1/1‑114 (MQ=255)                                                                                                                                             cgccaacaaaacgcaatggaagaccgcgaggcagcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcacccctaaatttagcgcttatgggaGATCCCCCCCCACCTTTCGCGCTCGCCTTCACGCAGGAAAACATACAGCCCGCCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGttt                                                                                                     >  1:227072‑M1/103‑251 (MQ=255)                                                                                                                                                        cGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgtaatgccggggctctaacccg                                                                                          >  2:180880‑M1/1‑95 (MQ=255)                                                                                                                                                            aTCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCtcccataagcgctaacttaag                                                                                                                                                                                                                                 <  2:54520‑M1/112‑22 (MQ=255)                                                                                                                                                            aTCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCtcccataagcgctaacttaag                                                                                                                                                                                                                                 >  1:54520‑M1/1‑91 (MQ=255)                                                                                                                                                                    acgcgagtcatcgtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggaGATCCCCCCCCCCCCTCCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGAc                                                                              >  1:191056‑M1/80‑251 (MQ=255)                                                                                                                                                                                gtcttaacgaggcaccgaggcgtcgcattcttcagatggttcaacccttaagttagcgcttatgggaGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTg                                                                   <  1:74375‑M1/183‑1 (MQ=255)                                                                                                                                                                                       ggCAAGCAGCACACTTTGAGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCccccataagcgctaacttaagggttggggtcttacgccggatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgtactgccggggctctaacccgccgccgcgaaattcgggatgctgcaacgctg                                                           >  2:101217‑M1/1‑64 (MQ=255)                                                                                                                                                                                         cAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgtaatgccggggctctaacccgccgccgcgaaattcgtgatgctgcaactctgct                                                         >  1:185249‑M1/1‑62 (MQ=255)                                                                                                                                                                                           agcagcTCACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgtaatgccggggctctaacccgccgccgcgaaattcgtgatgctgcaactctgctac                                                       <  2:196815‑M1/251‑192 (MQ=255)                                                                                                                                                                                               gcaCACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgtaatgccggggctctaacccgccgccgcgaaattcgtgatgctgcaactctgctacgtct                                                   >  1:66376‑M1/1‑56 (MQ=255)                                                                                                                                                                                                cacaCTTTGCGCCTCTTCCCAGGTTTCCTGACGGAAGCCCGGCAGTGGGGAGATCtcccataagcactaactgaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcccgatacctggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgtaatgccggggctctaacccgccgccgcgaaattcgtgatgctgcaactcggctacgtctg                                                  <  2:39541‑M1/251‑197 (MQ=255)                                                                                                                                                                                                     gtcgcattcttcagatggttcaacccttaagttagcgcttatgggaGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGAc                                             <  2:59159‑M1/205‑1 (MQ=255)                                                                                                                                                                                                          gcCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCtcccataagcgctcacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgtaatgccggggctctaacccgccgccgcgaaattcgtgatgctgcaactctgctacgtctggggctggctt                                        >  2:25850‑M1/1‑45 (MQ=255)                                                                                                                                                                                                                          accccttatgttaacgtttatggaaGCTCTCCCCCCCCCCTTCGCGCTCCCCTTCACGCAGCAAAACATCCGCCCCGTCGAGGGTTTTAAGCCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCTTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACa                        >  2:130080‑M1/26‑251 (MQ=255)                                                                                                                                                                                                                                              ggggAGATCtcccataagcgctaacttaagggttgggggattacgcctgatatgatttaacgtgacgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaggaactggatacttcggcacgtaatgccggggctctaacccgccgccgcgaaattcgtgatgctgcaactctgctacgtctggggctggcctacggcaccggggggctgtcattccgtgaagtcatct    >  2:50155‑M1/1‑9 (MQ=255)                                                                                                                                                                                                                                                ggAGATCtcccataagcgctaacttaagggttgtggtattacgcctgatatgatttaacgtgccgatgaattactctcacgataactggtcagcaattctggcccatattggtaagcccgaagaactggatacttcggcacgtaatgccggggctctaacccgccgccgcgaaattcgtgatgctgcaactctgctacgtctggggctggcttacggccccggggggatgtcattacgtgaagtcactgca  <  1:224856‑M1/251‑245 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        CCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGCCATTACACAGCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCAG  >  NC_000913/2375586‑2376075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.