Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,847,969	C→T	G14R (GGA→AGA)	selD ←	selenide, water dikinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,847,969	0	C	T	93.8%	48.8 / NA	16	G14R (GGA→AGA)	selD	selenide, water dikinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/6);  minor base G (0/1);  total (9/7)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTC  >  NC_000913/1847768‑1848182                                                                                                                                                                                                          |                                                                                                                                                                                                                      aaaaaTCAAAAGGATAATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCGGGGGCCATTGCCAAGAGCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTcc                                                                                                                                                                      <  2:271332/250‑1 (MQ=255)                 aTCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCgg                                                                                                                                                      >  2:253772/1‑251 (MQ=255)                            gCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAAcc                                                                                                                                           >  2:59501/1‑251 (MQ=255)                                      gTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGt                                                                                                                                 >  2:32207/1‑251 (MQ=255)                                                       cgctgggcccATTGCCAAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGagag                                                                                                                <  2:6397/243‑1 (MQ=255)                                                            gtcccATTCCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCt                                                                                                           <  2:201140/248‑1 (MQ=255)                                                                                           cgtcgCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATTCGGGTAGGGTAACCCTCATTCCGTTAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGTTCACAATCCAGa                                                                             <  2:149739/250‑1 (MQ=255)                                                                                           cgtcgCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGAc                                                                            >  1:139576/1‑251 (MQ=255)                                                                                                             ccACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATc                                                          >  2:287466/1‑251 (MQ=255)                                                                                                                           ggATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCCCAATCCCGACAGTTTCTCGACACTTATCAGAAATAAGTTAcc                                            >  2:13396/1‑250 (MQ=255)                                                                                                                                       ttCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCggg                                >  1:283508/1‑251 (MQ=255)                                                                                                                                           ccTGCTCACTATGCAGGATGGTTTCCAACACTTTTGTGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGa                                                                                                                                                                                                 <  1:201673/86‑1 (MQ=255)                                                                                                                                           ccTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGa                                                                                                                                                                                                 >  2:201673/1‑86 (MQ=255)                                                                                                                                           ccTGCTCACTAGGCAGGATGGTTTCCAACACGTTTGGGGAAATTTTACAGCCGCAACCAGCTCGGTGGCTGGATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCGGTGAAGGCAATCCGGGTAGGGTAACCATCAGTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCGTTGCTCACAGTCCAGACAGTTTCGCGACAAGTAGCAGAAATAAGTGACAAACGGCGTCGTGTCCg                            <  2:129833/251‑1 (MQ=255)                                                                                                                                                               gTTTCCAACACTTTGGGAGAAATTTTACAGCCGCAACCAGCGCTGTGGCTGTATTGGGGCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGGGATAAGGGAGAGTGTCTGAATTCCTGCTCCTTTGCGCACAATCCAGTCAGTTTCGCGACAATTATCAGAAATAAGTTACAAATGTCGTCGGGTCCGGGACTTTAATCGACGTAGAt        <  2:173016/251‑1 (MQ=255)                                                                                                                                                                      acacTTTTGGGGAAATTTTACAGCCGCAACCAGCTCTGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTc  >  1:280542/1‑250 (MQ=255)                                                                                                                                                                                                          |                                                                                                                                                                                                                      CAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTCGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTC  >  NC_000913/1847768‑1848182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑