Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,812,346	C→T	G263R (GGA→AGA)	ydjO ←	protein YdjO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,812,346	0	C	T	100.0%	55.7 / NA	18	G263R (GGA→AGA)	ydjO	protein YdjO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/9);  total (9/9)

GTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCC  >  NC_000913/1812102‑1812575                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      ggggtgtggactcTTTCGCCGCACGGATTGTTCTGCCGGCTTTGGGCCTGCCAGGAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTACCGACATGGGCCGTACGGCGTTAAACGTTAGTGTCTCGATGACAGCTGGCTCGCTGTCCAGCCAGTGGCTGAAGCAACCCGATAAAGCCATTCTTGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTg                                                                                                                                                                                                                                 <  2:36646/239‑1 (MQ=255)            aCTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGAAGACAGCTGGCACGCTGACCAGCCAATGGCTGAAGCAAAACGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAAATGGCACACCCTTAATTCTTTTGCTTGCCACGGTCTGTTTTGTATAGGGGCCg                                                                                                                                                                                                                      >  2:30747/1‑251 (MQ=255)                tCGCCTCACTGATGTTACTGCCGGCGATGGGCCTGCCATTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTg                                                                                                                                                                                                                   <  1:307763/250‑1 (MQ=255)                                 ctgcctgcGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTCTAGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGCCAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGCCGACGCCGAACCGGCCCACCATTAATTCTTATGCTGGCAAAGGTCTTTTTTTTATAGGGGCCGGTGCCTTACTTTTTAAATGtt                                                                                                                                                                                                 >  2:189958/1‑251 (MQ=255)                                                           ccTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTc                                                                                                                                                                        >  1:279105/1‑250 (MQ=255)                                                                       gTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACa                                                                                                                                                             <  1:109928/249‑1 (MQ=255)                                                                          aaTCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGAAGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATc                                                                                                                                                         <  1:30747/250‑1 (MQ=255)                                                                               ccGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTc                                                                                                                                                      <  2:55862/248‑1 (MQ=255)                                                                                 gTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGGCTGTTTTGTATAGGGGCCGTTGCCTTAATTTTTAAATGTTCCATCGCGGCGATGCCTACTGACTCATATTTAAACCGATAGTCAGCgg                                                                                                                                                 >  1:117878/1‑250 (MQ=255)                                                                                        cGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTTTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAAAAGATCGTCAGCGaaaaaaa                                                                                                                                           >  2:211627/1‑250 (MQ=255)                                                                                                              ggCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGCCCAGCCAGTGGCTGAAGCAAACCGATAAAGCCCTTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCCCGGCGATGCATAATGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATAAATACATTcc                                                                                                                    >  1:110520/1‑251 (MQ=255)                                                                                                                                                aCGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATAc                                                                                                                         >  1:259654/1‑212 (MQ=255)                                                                                                                                                aCGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATAc                                                                                                                         <  2:259654/212‑1 (MQ=255)                                                                                                                                                           ccagTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATAc                                                                       <  2:279105/251‑1 (MQ=255)                                                                                                                                                              gTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTaaaa                                                                               <  2:107999/240‑1 (MQ=255)                                                                                                                                                              gTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTaaaa                                                                               >  1:107999/1‑240 (MQ=255)                                                                                                                                                                                                                               aaTTCTTATGCTGGCAACGGTCTGTTTTGAATAAGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTcc   >  1:253745/1‑251 (MQ=255)                                                                                                                                                                                                                                aTTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTccc  <  2:114396/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      GTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCC  >  NC_000913/1812102‑1812575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑