Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,795,562	C→T	D795N (GAT→AAT)	pheT ←	phenylalanine‑‑tRNA ligase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,795,562	0	C	T	100.0%	73.8 / NA	22	D795N (GAT→AAT)	pheT	phenylalanine‑‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/14);  total (8/14)

CGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATCCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGAT  >  NC_000913/1795348‑1795773                                                                                                                                                                                                                       |                                                                                                                                                                                                                    cGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCct                                                                                                                                                                                  <  1:75723/250‑1 (MQ=255)              gTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCg                                                                                                                                                                    >  2:100556/1‑251 (MQ=255)                              tGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAAtct                                                                                                                                                    >  2:103998/1‑251 (MQ=255)                                      aTCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCtcttct                                                                                                                                            <  1:41433/251‑1 (MQ=255)                                                                               gTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATgg                                                                                                    <  1:181360/250‑1 (MQ=255)                                                                                            cAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTtattc                                                                                      <  2:163842/251‑3 (MQ=255)                                                                                                                 gAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACcg                                                                 >  1:8051/1‑251 (MQ=255)                                                                                                                            cAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTTACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATTGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTc                                                      <  1:42038/251‑1 (MQ=255)                                                                                                                            cAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGt                                                       <  1:103998/250‑1 (MQ=255)                                                                                                                                               ccGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCATCTACACATTTGGCGACGGTAGCGGCACTCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAAAACCCTTACCGCGGTACACGTCAAATAATTTTACGCCCACt                                   >  2:143521/1‑251 (MQ=255)                                                                                                                                                 gCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACAGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTAc                                 <  2:232228/251‑1 (MQ=255)                                                                                                                                                       tAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGAtt                           >  1:65523/1‑251 (MQ=255)                                                                                                                                                       tAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGAtt                           >  1:131078/1‑251 (MQ=255)                                                                                                                                                              aaGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCTTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTa                                           >  2:214213/1‑228 (MQ=255)                                                                                                                                                              aaGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTa                                           <  1:214213/228‑1 (MQ=255)                                                                                                                                                              aaGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCa                    <  1:100556/251‑1 (MQ=255)                                                                                                                                                                gCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAAc                  <  1:229052/251‑1 (MQ=255)                                                                                                                                                                      cAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCt             <  1:111986/250‑1 (MQ=255)                                                                                                                                                                             atatTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCg     <  1:48605/251‑1 (MQ=255)                                                                                                                                                                             atatTCTGACATTTCAGCGTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAAGCGCTCTTGTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTGCACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCg     <  2:261208/251‑1 (MQ=255)                                                                                                                                                                                                                gTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGAt  <  1:92884/219‑1 (MQ=255)                                                                                                                                                                                                                gTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGAt  >  2:92884/1‑219 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                    CGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATCCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGAT  >  NC_000913/1795348‑1795773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑