Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,790,949	C→T	G99D (GGC→GAC)	selO ←	protein adenylyltransferase SelO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,790,949	0	C	T	100.0%	80.5 / NA	25	G99D (GGC→GAC)	selO	protein adenylyltransferase SelO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/13);  total (12/13)

CCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTAT  >  NC_000913/1790709‑1791195                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       ccAAAGCGCACATGCCTTGTTGCCACACGCATCAGCATCTCGCCTGGCTCCCCCCTTTCCCAATACACTGGCTAACCGCTGGTGACGCTACTTAACGCGCGGGTCGTCGGAATGCCCCGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAACCCGCCCATCCATCCCCCATTCCCGAATAAGGCCTCAGGCCAGCACCTTTCAGATGCCATTCCATTGTATCGTCATCagca                                                                                                                                                                                                                                                >  2:272317/1‑249 (MQ=255)                          cacGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCcg                                                                                                                                                                                                                     >  2:77475/1‑251 (MQ=255)                                ccAGCATCGCGCCTGGCGCCGCCGTTGCCAGATACACTGGCGAAGCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCCCTGGCGTGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTGTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCa                                                                                                                                                                                                               <  2:303453/250‑1 (MQ=255)                                                                   acTGGCGAATCGCTGGTGACGATACTTAACGCGCCGGACGTCGGAATGCCCAGATAATGCATCGCCACACTGGCGACACTTTCTCGCATCGTCGAACGCAAAACCGCCCGTCCATCCCCCATTCGCGAATCAGCCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTCGGCCCGCCCAGACGCCGAACtgatg                                                                                                                                                                            >  1:289146/1‑251 (MQ=255)                                                                      ggCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGAc                                                                                                                                                                          <  1:126068/250‑1 (MQ=255)                                                                               cTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAAc                                                                                                                                                                >  1:278865/1‑251 (MQ=255)                                                                                      cGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGcc                                                                                                                                                         >  1:281083/1‑251 (MQ=255)                                                                                             tAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAgtgg                                                                                                                                                    <  2:97522/249‑1 (MQ=255)                                                                                              aaCGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAgtggtg                                                                                                                                                  <  2:100984/250‑1 (MQ=255)                                                                                                    cgGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACGGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGAGGCCAGTCCATGGTAGTGTCATCAGCAAGCAGTTGTGCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGTCCACTGTAAACCTGCGCCAGTGGTGACATatc                                                                                                                                           <  1:187296/251‑3 (MQ=255)                                                                                                            gggaagtcccAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCTAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTa                                                                                                                                   <  2:261557/244‑1 (MQ=255)                                                                                                             ggAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGt                                                                                                                                    >  2:115934/1‑249 (MQ=255)                                                                                                                            tGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGcccc                                                                                                                    >  1:199368/1‑250 (MQ=255)                                                                                                                               aTCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGac                                                                                                                >  2:124299/1‑251 (MQ=255)                                                                                                                                   ccTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGCGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCt                                                                                                            >  1:67534/1‑251 (MQ=255)                                                                                                                                    cTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTg                                                                                                           >  1:27059/1‑251 (MQ=255)                                                                                                                                     ccACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTATTCCTGCGCCAGTGGTCTCATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTgc                                                                                                          <  1:31828/250‑1 (MQ=255)                                                                                                                                      cACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTg                                                                                                           <  1:260016/249‑1 (MQ=255)                                                                                                                                                   ttCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAAc                                                                                             <  1:115934/250‑1 (MQ=255)                                                                                                                                                                           cGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCgtgt                                                                    <  2:278865/251‑1 (MQ=255)                                                                                                                                                                                      cACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCg                                                         >  1:31459/1‑251 (MQ=255)                                                                                                                                                                                                                                gCCAGTCCATTGTAGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAggg               <  2:27059/251‑1 (MQ=255)                                                                                                                                                                                                                                             aGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTaagatcggagagcacacgtctgaac  >  1:222011/1‑228 (MQ=255)                                                                                                                                                                                                                                             aGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTa                          <  2:222011/227‑1 (MQ=255)                                                                                                                                                                                                                                             aGTGTCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATATCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGta   <  2:303360/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       CCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAGGGGAAAGTGCTGTAT  >  NC_000913/1790709‑1791195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑