Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,743,164	C→T	E27K (GAG→AAG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,743,164	0	C	T	100.0%	69.4 / NA	21	E27K (GAG→AAG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/13);  total (8/13)

CAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGAATTAATAAATCCTCGCTACAATAGACTGAATTTCCCCT  >  NC_000913/1742921‑1743385                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                              cagcagTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTcaccac                                                                                                                                                                                                                         <  2:220095/250‑1 (MQ=255)  agcagTGCTCATAATATGAACTAACAGTACGTGCCAGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCACTCCCCCACTTTTAAATCGCCAAGATTGGTATTGCGTCACGTTTCTTTCATCAGGTCAAAACTGACCTGGTTGCCGTTAATTTCCGTCACGGTCATGCAGCAACCGTGATGCGCCAAGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGACGGGTAACTTCACCACAt                                                                                                                                                                                                                       >  2:131113/1‑251 (MQ=255)     agTGGTCATAATATGACCTGACATTAAGTGTACGCCAATTTCATCACTGAATTTCGCCGCACGCTAAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGAAATGCGCAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCCCCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCg                                                                                                                                                                                                                    >  1:201309/1‑251 (MQ=255)              aataTGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTGTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAAt                                                                                                                                                                                                           <  1:50159/251‑1 (MQ=255)                                         aaTTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGtt                                                                                                                                                                                <  2:237782/251‑1 (MQ=255)                                                 caCTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTg                                                                                                                                                                        <  1:241552/251‑1 (MQ=255)                                                     gAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTg                                                                                                                                                                             <  1:166195/242‑1 (MQ=255)                                                     gAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTg                                                                                                                                                                             >  2:166195/1‑242 (MQ=255)                                                       aTTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGt                                                                                                                                                                  <  2:93171/251‑1 (MQ=255)                                                                       aaCGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAAc                                                                                                                                                   >  1:177828/1‑250 (MQ=255)                                                                              aCCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTAc                                                                                                                                           >  2:190001/1‑251 (MQ=255)                                                                                ccAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGGTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGGTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCGCATGCGTACGAAAATTTGGTTTCTCGTCAACCGACACCAGTTTTGCGGTGCCCTGTACAATCGCCGTAAACATACTTACaa                                                                                                                                         <  2:201309/251‑1 (MQ=255)                                                                                                                                             aaaCTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCa                                                                            <  1:190001/251‑1 (MQ=255)                                                                                                                                                  gACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGaa                                                                                                                                                                                                              >  2:119843/1‑116 (MQ=255)                                                                                                                                                  gACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGaa                                                                                                                                                                                                              <  1:119843/116‑1 (MQ=255)                                                                                                                                                     aTGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAggg                                                                    <  1:168932/251‑1 (MQ=255)                                                                                                                                                                 aaTTTCCGTCAAGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCt                                                        >  1:261382/1‑251 (MQ=255)                                                                                                                                                                      ccGTCACGGTCAGGCAGCAACCGTGATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTAtttt                                                   <  1:32293/251‑1 (MQ=255)                                                                                                                                                                          cACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTc                                                <  1:56786/250‑1 (MQ=255)                                                                                                                                                                                                        ggAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGGGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGAATTAATAAATCCTCGCTACAATa                 <  2:150537/251‑1 (MQ=255)                                                                                                                                                                                                                       cAGGCCGTCCAGACTGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGAATTAATAAATCCTCGCTACAATAGACTGAATTTCCCCt  >  2:296367/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                              CAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGAATTAATAAATCCTCGCTACAATAGACTGAATTTCCCCT  >  NC_000913/1742921‑1743385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑