Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,587,323	C→T	A138T (GCC→ACC)	ydeQ ←	putative fimbrial adhesin protein YdeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,587,323	0	C	T	100.0%	20.4 / NA	8	A138T (GCC→ACC)	ydeQ	putative fimbrial adhesin protein YdeQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/3);  total (5/3)

AGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGTCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGCCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATGACTGTAGGGAGCCTGCGAAAGCTGAGCCTTGTACCAGGTTTATATGATCAGTGTCGTACCAGCCG  >  NC_000913/1587083‑1587522                                                                                                                                                                                                                                                 |                                                                                                                                                                                                        agagCTTGTGTTCGCTTGAGCAATATACGCCAAGGGGTAGTTCTGCGCGTCCAGGAAAGTCCGTGAGATCCACGGTTACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAGCAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGTTTTGCGCGTCCGAATGTTGCAATTTTATACATATGGATGGGGGCAATGGCGTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACt                                                                                                                                                                                               <  1:273810/251‑1 (MQ=255)          gTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGTCCGGGAGATCCACGGTGACATGTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGTGTAat                                                                                                                                                                                       <  2:126919/250‑1 (MQ=255)                 tGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGTCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCACCAACTGGGGTAATATAGAGtt                                                                                                                                                                               >  1:159802/1‑251 (MQ=255)                                                                                                          cTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTcc                                                                                        >  2:115631/1‑249 (MQ=255)                                                                                                                      caacaTTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCACCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCt                                                                          >  1:203297/1‑251 (MQ=255)                                                                                                                                         aataTTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATGACTGTAGGGAGc                                                       >  2:62608/1‑251 (MQ=255)                                                                                                                                         aagatTCCACGTAAAATTACGCGGATTTCCGCTTACTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGGGTCTTATCACCAATATCCAATACGTGGGTATTTGTCGTAAACGGGAAGGGGT‑GGTCACATTGTTCCAGTACAGCTATCCTTTATATGACTGTAGGGAGcc                                                      <  2:137531/248‑1 (MQ=255)                                                                                                                                                                                              aCATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGTCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATGACTGTAGGGAGCCTGCGAAAGCTGAGCCTTGTACCAGGTTTATATGATCAGTGTCGTACCAgccg  >  1:114355/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                        AGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCCAGGAAAGTCCGGGAGATCCACGGTGACATTTCGACTATCAACGGTGCAGCCGCCTGTTGGCATAACAACATTGTTATTTGAAATAATATTCCACGTAAAATTACGCGGATTTCCGCTTCCTAATGTTGCAATTTTATACATATGAATACGGGCAATGACTTCCCCCGCTTTTATGACAACTCCACCGGCCGCCCCAACTGGGGTAATATAGAGTTTTAACGGTAATGGCATCGGTGTCTTATCACCAATATCCAATACGTTGGTATTTGTCGTCAACGGGAAGGGGTAGGTCACATTATTCCAGTACAGCGATCCTTTATATGACTGTAGGGAGCCTGCGAAAGCTGAGCCTTGTACCAGGTTTATATGATCAGTGTCGTACCAGCCG  >  NC_000913/1587083‑1587522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑