Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	88,571	G→A	V182M (GTG→ATG)	cra →	DNA‑binding transcriptional dual regulator Cra

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	88,571	0	G	A	100.0%	141.7 / NA	42	V182M (GTG→ATG)	cra	DNA‑binding transcriptional dual regulator Cra
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/21);  total (21/21)

AGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTCGCGACGGC  >  NC_000913/88324‑88813                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   agaagaTCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGc                                                                                                                                                                                                                                                 >  2:256409/1‑251 (MQ=255)    agaTCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCTTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGCCCCGTT‑CCCGATTTTCGCCCTGGACC‑GCGCCCTCGATCGTGACCACTTCCCCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAAGGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCttt                                                                                                                                                                                                                                              <  1:133976/251‑1 (MQ=255)            cAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTggg                                                                                                                                                                                                                                      >  2:159530/1‑250 (MQ=255)            cAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGt                                                                                                                                                                                                                                      >  1:96992/1‑251 (MQ=255)                 aaCGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGCCGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCt                                                                                                                                                                                                                                 >  1:34953/1‑251 (MQ=255)                   cGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCAGAGACGATGCTTTATCTTGGTGCGCTAc                                                                                                                                                                                                                               >  1:159643/1‑251 (MQ=255)                          cGGTGCATTGCGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGGGCt                                                                                                                                                                                                                        >  1:54345/1‑251 (MQ=255)                             tGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTc                                                                                                                                                                                                                     >  1:276335/1‑251 (MQ=255)                                                     cagttggATGCCATTAGTGTGTCGACGGCGGCCCCTCCGGAGCACCCTTTTTAACAACGCGGGGCTACCGCCCCTTC‑CCCGATTGTCGGGC‑GGACCCGCGCCATCGTTGGGGAACCTTTCACCAGCGTGGTTGGTGCGGATCAGGATGCTGCCGAAAGGCGGGGGGAAGAGTTCCGTAAGTTTTCCTCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAgg                                                                                                                                                                                             <  2:144531/245‑1 (MQ=255)                                                     cAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCTTCCTTTTTATCAACGCTGGGCTACCGACCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAgg                                                                                                                                                                                             <  1:119331/251‑1 (MQ=255)                                                       ggTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGtt                                                                                                                                                                                           >  2:49807/1‑251 (MQ=255)                                                         tgtaTGCCATTATTGTTTCGGCGTCGTTGCCCCCGGAGCCTCCTTTTTACCAACGCGGGGCTAACGCCCCGTT‑CCCGTTTGTTGCGCTGGCCC‑GCGCCCTCTATCGTGAACACTCCCCCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTCCGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGttt                                                                                                                                                                                          <  2:42041/247‑1 (MQ=255)                                                            aTGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGt                                                                                                                                                                                      >  1:246945/1‑251 (MQ=255)                                                                cATTATTGTTTCGACGTCGTTGCCTCCTGACCATCCTTTTTATCACCGCTGGGCTAACG‑CCCGTTTCCCGATTGTCGCGCTGGACG‑GCGCCCTCGATCGGGACCACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTCCGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTg                                                                                                                                                                                  <  1:241764/251‑1 (MQ=255)                                                                  ttattGTTTCGACGTCTTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTT‑CCCGATTGTCGCGCGGGCCC‑GCGCCCTCGATCGTGAACACTTCCCCAGCGGGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGc                                                                                                                                                                                 <  2:57515/250‑1 (MQ=255)                                                                            gACGTCGTTGCCCCCTGAGCATCCTTTTTACCAACGCTGGGCTAACGCCCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACATTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAATTGCTGGGGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTGCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAgatg                                                                                                                                                                      <  2:276335/251‑1 (MQ=255)                                                                             aCGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAgatg                                                                                                                                                                      >  1:99334/1‑250 (MQ=255)                                                                                       ctcctGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCAGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGa                                                                                                                                                            >  2:79231/1‑250 (MQ=255)                                                                                               cATCCTTTTCATCAACGCCGGGATAAAGACCCGAT‑ACCGATTGTCGCGCTGGACA‑GCGACCTCGATAGTGAACACTTCACCAGCGTGGTTGGTCCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGCCAGTTTCCCGCCGAGACGATGCCTTATCTTGGTGCGCTACCGGACCTTTCTGTCAGCTTCCTACGTGAACAAGGTTCCCGTACTGCTTGGAAAGATGATCCGCGCGAAGTGCAttt                                                                                                                                                   >  2:277454/1‑251 (MQ=255)                                                                                                          caaaCGCTGGGCTAACGCCCCGTT‑CCCGATTGTCGCGCGGGACC‑GCGCCCTCGATCGTAAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGGGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCa                                                                                                                                        <  2:96992/249‑1 (MQ=255)                                                                                                             ccGCTGGGCTAACGCCCCGTT‑CCCGATTGGGGCGCTGGCCC‑GCGCCCTGGATCGTAAACCCTTCACCAGCGTGGTTGGTGCGGATCAGGATGATGCCAAAATGCTGGGGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACa                                                                                                                                     <  2:276724/250‑1 (MQ=255)                                                                                                             aCGCTGGGCTAACGACCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAAc                                                                                                                                      >  1:255598/1‑250 (MQ=255)                                                                                                                 tGGGCTAACGACCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAgat                                                                                                                                                                       >  1:91907/1‑213 (MQ=255)                                                                                                                                          gTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCAAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGtt                                                                                                         <  1:175203/251‑1 (MQ=255)                                                                                                                                                                    gggaaccctgcACCGGCGTGGTTGGTGCGGGTCGGGATGAGGCCGAAATGGTGGCGGAGGAGTTACGAAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACCGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCAt                                                                                <  2:244318/241‑1 (MQ=255)                                                                                                                                                                      aaccacTTCCCCTGCGTGGTTGGTGCCGATCAGAATGATCCCGAAATCCGGGGGAAATATTTCCGTAGGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATcc                                                                              <  2:94066/248‑1 (MQ=255)                                                                                                                                                                                  aGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGAAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGgc                                                                  <  1:216800/251‑1 (MQ=255)                                                                                                                                                                                   gggtggtgGGTGCAGATCAGGATGATGCCGAAATGCTGTGGGAAGAGTTACGTAAGTTTGCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGgcg                                                                 <  1:186591/249‑1 (MQ=255)                                                                                                                                                                                     gTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCt                                                               <  1:228694/251‑1 (MQ=255)                                                                                                                                                                                          tggtGCCGTTCAGGATGATGCCGAAATGCTGGCGGAGGAGTTACGTAGTTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTc                                                           <  1:233374/250‑1 (MQ=255)                                                                                                                                                                                                        gtgatgCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCg                                            <  2:70396/250‑1 (MQ=255)                                                                                                                                                                                                            tgCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCgttgt                                        >  1:9284/1‑251 (MQ=255)                                                                                                                                                                                                                      cTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAgg                              >  2:165943/1‑250 (MQ=255)                                                                                                                                                                                                                            gAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTgatgga                        <  1:114048/251‑1 (MQ=255)                                                                                                                                                                                                                                 gTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTgatggatg                      >  2:251988/1‑248 (MQ=255)                                                                                                                                                                                                                                 gTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTgatggatg                      <  1:251988/248‑1 (MQ=255)                                                                                                                                                                                                                                   tACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACg                 >  1:209369/1‑251 (MQ=255)                                                                                                                                                                                                                                     cGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCt               >  1:305683/1‑251 (MQ=255)                                                                                                                                                                                                                                          gTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTc          >  1:112041/1‑251 (MQ=255)                                                                                                                                                                                                                                          gTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGGTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTc          >  2:154231/1‑251 (MQ=255)                                                                                                                                                                                                                                              cccGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCGTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAATGAGTGATGGATGTCACGCTGCGTcgcg       <  1:81675/250‑1 (MQ=255)                                                                                                                                                                                                                                                  ccgAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTCGCGACGGc  <  2:112041/251‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   AGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTT‑CCCGATTGTCGCGCTGGACC‑GCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTCGCGACGGC  >  NC_000913/88324‑88813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑