Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,520,526	C→T	R89C (CGC→TGC)	mcbR →	DNA‑binding transcriptional dual regulator McbR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,520,526	0	C	T	100.0%	38.0 / NA	13	R89C (CGC→TGC)	mcbR	DNA‑binding transcriptional dual regulator McbR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/7);  total (6/7)

TGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAGGTATGAGTATTACACCTGTGCGTGAAGCATTATTACGTCTGGTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTG  >  NC_000913/1520309‑1520735                                                                                                                                                                                                                          |                                                                                                                                                                                                                  tGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAGGTATGAGTATTACACCTGTGCGTGAAGCATTATTACGTCTGGTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCTGCTACGAACTGGAATTAATGGCAGTTGCTCTg                                                                                                                                                                                   <  2:44361/250‑1 (MQ=255)  gagaACGACCTGAAACATCAGCTTAGTCTTCGCGCATTAAACCCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAGGTATGAGTATTACACCTGTGCGTGAAGCATTATTACGTCTGGTTTCGGTGAATGCGCTTTCTGTCGCACCTGCCCAAGCATTTACAGTTCCGGAAGGGGGGAAACGTCACTTAGACGAAATCAATCGGATCTGCTACGAACTGGAATTAATGGCAGTTGATCTGGc                                                                                                                                                                                 >  2:132070/1‑251 (MQ=255)         aCCTGAAACATCAGCTTAGTATTGGCGCATTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAGGTATGAGTATTACACCTGTGCGTGAAGCATTATTACGTCTGGTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCTGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGa                                                                                                                                                                           >  1:77511/1‑250 (MQ=255)              aaaCATCAGCTTAGTATTGGCGAATTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAGGTATGAGTATTACACCTGTGCGTTAAGCATTATTACGTCTGGTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCTGCTCCGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAATCCt                                                                                                                                                                     <  2:170101/251‑1 (MQ=255)                                 gcgtaTTAAAACCTGGCGCACGCCTGATTAATAAAAATCTGGCGGAGCAATTAGGTATGAGTATTACACCTGTGCGTGAAGCATTATTACGTCTGGTTTCGGTGAATGCGCTTTCTGTCGAATCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCTGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCg                                                                                                                                                  <  2:255350/247‑1 (MQ=255)                                                                                             gTATTACACCTGTGCGTGAAGCATTATTACGTCTGGTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCTGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaa                                                                                      <  2:222175/251‑1 (MQ=255)                                                                                                   cacCTGTGCGTGAAGCATTATTACGTCTGGTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCTGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCAtt                                                                                <  1:219480/251‑1 (MQ=255)                                                                                                                            tatGGTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCTGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCtt                                                         >  2:65205/3‑249 (MQ=255)                                                                                                                                   tCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGTGGAAACGTCAATTAGACGAAATCAATCGGATCTGCTACGAACTGGAATTAATGGCAGTTGCTCTGTCTGTTGAAAACCTCACCCCTCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAttt                                                 <  1:23250/250‑1 (MQ=255)                                                                                                                                             cgcTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCTGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTc                                       >  1:225798/1‑250 (MQ=255)                                                                                                                                                          aCCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTAAATTAGACGAAATCAATCGGATCTGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCAGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGAAATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGAGATTTATCATCGCTCAAATATGCCCATcc                         >  2:307652/1‑251 (MQ=255)                                                                                                                                                                                ccGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCTGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACt   <  1:231917/251‑1 (MQ=255)                                                                                                                                                                                 cGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCTGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACtg  >  2:78841/1‑251 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                  TGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAGGTATGAGTATTACACCTGTGCGTGAAGCATTATTACGTCTGGTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTG  >  NC_000913/1520309‑1520735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑