Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,514,985 C→T A75V (GCG→GTG)  ydcV → putative ABC transporter membrane subunit YdcV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,514,9850CT93.3% 32.4 / ‑6.0 15A75V (GCG→GTG) ydcVputative ABC transporter membrane subunit YdcV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (5/9);  minor base G (1/0);  total (6/9)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGTCTGGGAGCGTTCGATGCACTCTGAACGCGCACCGTTTTTCCTCAAACTGGCGGCCTGGGGCGGCGTTGTTTTCCTACATTTTCCCATCCTGATAATCGCCGCCTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATC  >  NC_000913/1514744‑1515220
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           
aaCGTCTGGGAGCGTTCGATGCACTCTGAACGCGCACCGTTTTTCCTCAAACTGGCGGCCTGGGGCGGCGTTGTTTTCCTACATTTTCCCATCCTGATAATCGCCGCCTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAAt                                                                                                                                                                                                                                    >  1:14080/1‑251 (MQ=255)
                           gAACGCGCACCGTTTTTCCTCAAACTGGCGGCCTGGGGCGGCGTTGTTTTCCTACATTTTCCCATCCTGATAATCGCCGCCTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATAGTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCa                                                                                                                                                                                                           <  2:156047/249‑1 (MQ=255)
                             aCGCGCACCGTTTTTCCTCAAACTGGCGGCCTGGGGCGGCGTTGTTTTCCTACATTTTCCCATCCTGATAATCGCCGCCTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTg                                                                                                                                                                                                       <  2:174491/251‑1 (MQ=255)
                                                         gcCTGGGGCGGCGTTGTTTTCCTACATTTTCACATCATGAGAGTAGCCGCCTATACCTTCAACACTGAAGCTACGGCGTTTAGTTTTACACCGCAGAGCATGCCGCCGCGCTGGTTTAGCGTGGCAGCACAGAGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGGGCTGGTGACATTAATTGCGCTGGTGTTAGGGCCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCgg                                                                                                                                                                           >  1:192331/1‑251 (MQ=255)
                                                                   gcgTTGTTTTCCTACATTTTCCCATCCTGATAATCGCCGCCTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGcc                                                                                                                                                                 <  2:263661/251‑1 (MQ=255)
                                                                                                                            aaGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCg                                                                                                        <  2:14080/251‑1 (MQ=255)
                                                                                                                                    gcgTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGGGACATTAATTGCGCTGGGGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTTTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGGGTTATTAAc                                                                                                >  2:223926/1‑251 (MQ=255)
                                                                                                                                                 cACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGGGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTATCACTGGTCTGGCGTTATTAACCGCCTTTAAAAcc                                                                                   <  1:199209/251‑1 (MQ=255)
                                                                                                                                                                                 gTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGCCTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGt                                                   <  2:76427/251‑1 (MQ=255)
                                                                                                                                                                                   ggCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTgg                                                 <  1:104211/251‑1 (MQ=255)
                                                                                                                                                                                           aGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCAt                                         >  1:60122/1‑251 (MQ=255)
                                                                                                                                                                                                    atatTCTTGATGCCGTGACTCTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGGGTTAGTGACGCTGGCAGCTGCCGCGCTGGGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCGGTTACTGCTGCTGCCCATTGCGCGGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACtttt                                <  1:274436/251‑1 (MQ=255)
                                                                                                                                                                                                            gATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGt                        >  1:279684/1‑251 (MQ=255)
                                                                                                                                                                                                               gCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGTGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTtgt                     >  1:292884/1‑251 (MQ=255)
                                                                                                                                                                                                                                  aaGTTGCGGCGCTGGTTGCATGAATTGCGCTGGTGTGAGGGCCGCTGGGATCTGCGGCGCTGTGGCGACGAGCCTTTTTCGGCAAAAACGCCATTTCGCTGTTTCTGCTGCTGCCCATTGCGCGGCCGGGCATTGTCACTGGTTGGGCGTTATTAGCCGCCGTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCTTGGTCGGTCATGCGACTTGTGGTTTAGTTGTGGTGTTTAACAATGTCATc  <  2:277963/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           
AACGTCTGGGAGCGTTCGATGCACTCTGAACGCGCACCGTTTTTCCTCAAACTGGCGGCCTGGGGCGGCGTTGTTTTCCTACATTTTCCCATCCTGATAATCGCCGCCTATGCGTTTAACACTGAAGATGCGGCGTTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATC  >  NC_000913/1514744‑1515220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑