Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,491,105	C→T	T68T (ACC→ACT)	cybB →	superoxide oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,491,105	0	C	T	100.0%	47.8 / NA	17	T68T (ACC→ACT)	cybB	superoxide oxidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/10);  total (7/10)

TTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTTTGGTTTGACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGT  >  NC_000913/1490864‑1491341                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             ttATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCACGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTCTCGCAGCGTATTGCGCACTGGAGTTTCGTGGTTTATTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTAGTCTTTTGTTAAGGCTGTAATACCCAACTCCGCAGAtt                                                                                                                                                                                                                                     >  1:144835/1‑251 (MQ=255)         acgtaaaaTTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGGTAGCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTGCTTCCCACTTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCGCGTGCTGATGGTCTGTCGTCTTGTTTTAAGGCTGAAATACCCATGTCCGCCGATTATACCTaa                                                                                                                                                                                                                             <  2:29848/247‑1 (MQ=255)                                         ggAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCTTGGTTTCTTCCCACGTAGCGATCGGCCGCTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCAt                                                                                                                                                                                              <  2:119941/250‑1 (MQ=255)                                                           gTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCCATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGGGAttt                                                                                                                                                                           >  2:3207/1‑251 (MQ=255)                                                            ttACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCAACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGCGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGGGAttt                                                                                                                                                                           >  1:27338/1‑250 (MQ=255)                                                                                         tttACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGAtt                                                                                                                                              <  2:295074/250‑1 (MQ=255)                                                                                                gTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGAttggtttggt                                                                                                                                      <  2:157372/251‑1 (MQ=255)                                                                                                        aGCGTATTTCGAAATGGAGTTTCGTGGTTTCTTACCATGTAGCGATCGGCCACTACTCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGGGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTACGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGACAGTGATTGGTTTTGTGatgatgg                                                                                                                              >  2:129490/1‑250 (MQ=255)                                                                                                                    aaTGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTg                                                                                                                                                       <  1:220068/214‑1 (MQ=255)                                                                                                                    aaTGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTg                                                                                                                                                       >  2:220068/1‑214 (MQ=255)                                                                                                                                                    aTCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCCTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTTTGGTTTGACGATGCCt                                                                                  >  1:26764/1‑251 (MQ=255)                                                                                                                                                        gCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTTTGGTTTGACGATGCCTTACg                                                                              <  1:171825/251‑1 (MQ=255)                                                                                                                                                          cACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTTTGGTGTGACGATGCCTTACGCt                                                                            <  2:13575/251‑1 (MQ=255)                                                                                                                                                              catcaACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTTTGGTTTGACGATGCCTTACGCTTc                                                                          <  1:36923/249‑1 (MQ=255)                                                                                                                                                                                                            agggTCGTTCGTATTTTGTTAAGGCGGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTTCTGTTTAGTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTGTGGTTTGACGATGCCTTACGCTTCAGAGGCCAAGTTCGAACGGGTAGAGAGCTTAAAGTCGTGGCATGAAAc                          <  2:38450/249‑1 (MQ=255)                                                                                                                                                                                                                  gttcgtCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTGTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTTTGGTTTGACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCAGGTAACGCTGGc                    <  2:126971/251‑1 (MQ=255)                                                                                                                                                                                                                                    cTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTTTGGTTTGACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGt  >  1:88160/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             TTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTTTGGTTTGACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGT  >  NC_000913/1490864‑1491341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑