Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,460,345	C→T	A431V (GCC→GTC)	paaH →	3‑hydroxyadipyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,460,345	0	C	T	100.0%	99.2 / NA	30	A431V (GCC→GTC)	paaH	3‑hydroxyadipyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/9);  total (21/9)

GACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGT  >  NC_000913/1460109‑1460574                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                      gACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTgggg                                                                                                                                                                                                                                 <  2:282765/243‑1 (MQ=255) gACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTgggg                                                                                                                                                                                                                                 >  1:282765/1‑243 (MQ=255) gACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAAc                                                                                                                                                                                                                         >  2:51516/1‑251 (MQ=255) gACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAAc                                                                                                                                                                                                                         >  2:51506/1‑251 (MQ=255)                   ggTGAACCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAatat                                                                                                                                                                                                       >  2:108791/1‑251 (MQ=255)                    gTGAACCCGGACTCAGCGACCCGCAAGGCCATTTCTTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATAtt                                                                                                                                                                                                      >  1:26077/1‑251 (MQ=255)                       aaCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTa                                                                                                                                                                                                     >  1:127238/1‑249 (MQ=255)                         cccGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTTATTTGGCGAACGGTAGCAATGATCATCAATGAAGCACTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTTGGGAGCGCAACTTGGCTGGCAGCGTATATTAAGGc                                                                                                                                                                                                 <  2:185701/251‑1 (MQ=255)                                 cAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGa                                                                                                                                                                                          >  1:294271/1‑250 (MQ=255)                                                       ttaCCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCg                                                                                                                                                                   >  2:230879/1‑251 (MQ=255)                                                             gCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCgaaga                                                                                                                                                               <  1:75291/249‑1 (MQ=255)                                                               aaCAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGc                                                                                                                                                           >  1:238172/1‑251 (MQ=255)                                                                                                      cAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTg                                                                                                                    <  1:108791/251‑1 (MQ=255)                                                                                                         gAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTgg                                                                                                                   >  2:222712/1‑249 (MQ=255)                                                                                                           aTGCTGATTTGGCGAACGGTAGCAATGATCATCCATGAAGCCCTTGATGCGCTTCACAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGgag                                                                                                                 >  2:179806/1‑249 (MQ=255)                                                                                                           aTGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGgag                                                                                                                 >  2:88175/1‑249 (MQ=255)                                                                                                           aTGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGagag                                                                                                               <  2:15486/251‑1 (MQ=255)                                                                                                                                 cAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTg                                                                                         >  2:236351/1‑251 (MQ=255)                                                                                                                                        catcaATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAAt                                                                                  >  1:150633/1‑251 (MQ=255)                                                                                                                                         atcaATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATg                                                                                 <  2:61687/251‑1 (MQ=255)                                                                                                                                            aaTGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGccc                                                                              >  1:229579/1‑251 (MQ=255)                                                                                                                                                                tCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGAt                                                          >  1:136091/1‑251 (MQ=255)                                                                                                                                                                             gCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCGTGCGCCaa                                               >  1:132182/1‑249 (MQ=255)                                                                                                                                                                                   gAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCt                                         <  1:17174/249‑1 (MQ=255)                                                                                                                                                                                       atgATATCGATACCGCCATGCGGCTTGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCAt                                   <  2:101467/249‑1 (MQ=255)                                                                                                                                                                                                                tGGGGTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAg            <  2:15304/249‑1 (MQ=255)                                                                                                                                                                                                                  gggTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCtt        >  1:61956/1‑251 (MQ=255)                                                                                                                                                                                                                    gTGAATTATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTAATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCATGGCATCGACATTATCTCAATGGATGAAGGCTTTg      >  1:8588/1‑251 (MQ=255)                                                                                                                                                                                                                          tATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGt  >  1:23707/1‑249 (MQ=255)                                                                                                                                                                                                                          tATCCATATGGCCCACTTGTCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGt  >  1:209205/1‑249 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                      GACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGT  >  NC_000913/1460109‑1460574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑