Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,453,992	G→A	W22* (TGG→TGA)	paaA →	phenylacetyl‑CoA 1,2‑epoxidase, monooxygenase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,453,992	0	G	A	100.0%	47.6 / NA	16	W22* (TGG→TGA)	paaA	phenylacetyl‑CoA 1,2‑epoxidase, monooxygenase subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/4);  total (12/4)

ACATTAATGCACTGATAAATAATGATTTATAAAAATAGGGTGCGAAATCCGTCACAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGGATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAA  >  NC_000913/1453759‑1454212                                                                                                                                                                                                                                          |                                                                                                                                                                                                                             aCATTAATGCACTGATAAATAATGATTTATAAAAATAGGGTGCGAAATCCGTCACAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCg                                                                                                                                                                                                             >  1:90316/1‑251 (MQ=255)      aaTGCACTGATAAATAATGATTTATAAAAATAGGGTGCGAAATCCGTCACAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGaa                                                                                                                                                                                                           >  2:224117/1‑248 (MQ=255)                                                             aaaCATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGAGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCACGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCAAGATTGGGCAGCATGCGCACTCCGAGCTTGTTGGTATGTTGcc                                                                                                                                                 >  1:26463/1‑251 (MQ=255)                                                                   aCAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAgg                                                                                                                                           >  1:184517/1‑251 (MQ=255)                                                                       aaTTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAAc                                                                                                                                       >  2:288608/1‑251 (MQ=255)                                                                              gATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATc                                                                                                                                 >  2:124002/1‑250 (MQ=255)                                                                                    aCTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTcgcg                                                                                                                          <  1:124002/251‑1 (MQ=255)                                                                                                 gtAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTgcg                                                                                                             <  1:42605/251‑1 (MQ=255)                                                                                                              aaCAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCa                                                                                                                     <  1:65062/230‑1 (MQ=255)                                                                                                              aaCAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCa                                                                                                                     >  2:65062/1‑230 (MQ=255)                                                                                                               aCAATGTGATTCGTTTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTc                                                                                               <  1:257376/251‑1 (MQ=255)                                                                                                                           gtgtTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCaa                                                                                     >  1:72993/1‑249 (MQ=255)                                                                                                                                                      gAATCGTTAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACATTGCGGCGTAAAGCCATTCTGTTGGCCACAGTGCAGGATGAAGCCGGTCATGGTCTGt                                                        >  2:132068/1‑251 (MQ=255)                                                                                                                                                                   gatgaCGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAgcgc                                           >  1:183211/1‑251 (MQ=255)                                                                                                                                                                                aagaagAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTg                              >  2:126444/1‑251 (MQ=255)                                                                                                                                                                                                             cAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCaaa  >  1:202181/1‑250 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                             ACATTAATGCACTGATAAATAATGATTTATAAAAATAGGGTGCGAAATCCGTCACAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGGATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAA  >  NC_000913/1453759‑1454212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑