Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,419,414	C→T	G15S (GGT→AGT)	ydaG ←	uncharacterized protein YdaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,419,414	0	C	T	100.0%	22.1 / NA	11	G15S (GGT→AGT)	ydaG	uncharacterized protein YdaG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/6);  total (5/6)

CTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCA  >  NC_000913/1419171‑1419653                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                cTTCTCCAACCATCGGCGCGCCCCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTAATGGCAGTGAACGTTCCATCCTGGTTGGGGACCCCGCCGCACACCAGGGATTCTTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTAACATCTTGTTGCTACGTCTAGCGACTTCTGCATGCCAGAGATCACAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGcataa                                                                                                                                                                                                                                           >  2:141639/1‑247 (MQ=255)     tcCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCATTTGGTATACGTCATGGCAGTGAACGTTCCATCCTAGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTGGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCGACGTATAGCGACTTCTGCCTGCCAGAGAACCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGGTGTGAGTGATACTGCAACAATcc                                                                                                                                                                                                                                       >  1:21830/1‑250 (MQ=255)                  gcgcACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAac                                                                                                                                                                                                                         >  1:235810/1‑251 (MQ=255)                                                       ataCGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACAAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACg                                                                                                                                                                                    >  1:35952/1‑251 (MQ=255)                                                                                                       cacCAGGGATTCGTTGTGGCGGAGTTCTTTTTTTTGCTTTTTGCGAATCTCACATCTTGTTGCTACGTATGGCGTCTTCTGCCGGCAAGAGACCCCAGTCTTTGCTGCGAAAAGCCTGCACAGCCTTGTTGAAAGTGATCCTGCAAAAATCCATAAAATACGGACCTCCTTCGTAATGCCCCATCTTACCTCCCCCCTTACCGCCGGGTGGCGAAACTAActgctgaaagtaaaaattggatgcccgccgt                                                                                                                                    <  2:68996/251‑26 (MQ=255)                                                                                                                           cGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGggc                                                                                                                >  1:146978/1‑251 (MQ=255)                                                                                                                                                          agatattgtttCTACGTATAGCGATTTCTGCCCGCCCGAGACACCAGTTGTTGCTCCGTAAAGCCTGCACATCCTGTTTGTAAGTGATACTTCAACAATCCATCAATTTCTTATCTACTTCGTAAGGCACCATCTTATCTCTCCCTTTAACGCCGGGTGGCGGAACTACCTGTTGCACTGCAAAATTTGAACCCCGCCGTCATGTTCATACACCTCGTGCTTGCTACTTAACCCCTTACCACTGCCTGGTa                                                                                 <  1:135215/246‑1 (MQ=255)                                                                                                                                                                                              agataTCCCAGCCGTTGCTGCGTAAATCCTGCACAGCCTGGTTGTAAGTGATACTGCAAAAATCCATCAAATCCTGAACTACTTCGTAAGGCACCATCTTATCTCTCCCCTTACCGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGgggt                                             <  2:87940/247‑1 (MQ=255)                                                                                                                                                                                                                     aaaGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACAGAACTACTTCGTAATGCCCCATCTTATCGCTCCCCTGAACGCCGGGTGGCGGAACGAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGATGGAACTATAg                       <  2:209671/250‑1 (MQ=255)                                                                                                                                                                                                                         ccTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAAt                  <  1:100146/251‑1 (MQ=255)                                                                                                                                                                                                                                         gTAAGTGATACTGCAACAATCCATCAAATACGTAACTGCTGCGTAATGCACCATCTTAGCTCGCCCCTTAACGCCGTGTGGCGGAACGAACTGCTTCACGTCAAAATTTGAACCCCGCCGTCATGTTCATACGCCTCGTGCTGGCTACTTAACCCCTTACCACGGCCGGGTAACTCGAAGTATTGCCCGGCGTTCGGTTGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCa  <  1:192997/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                CTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCA  >  NC_000913/1419171‑1419653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑