BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,418,807	C→T	T46I (ACT→ATT)	sieB →	phage superinfection exclusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,418,807	0	C	T	100.0%	59.5 / NA	19	T46I (ACT→ATT)	sieB	phage superinfection exclusion protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (10/9); total (10/9)

GTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTGAGAGTTATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATACTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGTATTTAAAGATTTCCTTAAAACAGGAAATCTTATTATCACTTCTCCTTGCCGTAACCCGGTTATGAAAAAATTAGAACGGAAG  >  NC_000913/1418563‑1419042

gtgCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTGAGAGTTATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAAt                                                                                                                                                                                                                                       >  2:138570/1‑251 (MQ=255)

         tttATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTGAGAGTTATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATGCCTTGGc                                                                                                                                                                                                                               <  1:112656/250‑1 (MQ=255)

            aTTTCCCCCCCTCCGGGCTTCGGTGGTCTCGGCTATCCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTGAGAGTTATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCtt                                                                                                                                                                                                                           <  1:285003/251‑1 (MQ=255)

            aTTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTGAGAGTTATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGctct                                                                                                                                                                                                                            <  2:89359/250‑1 (MQ=255)

                          gggCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCATTCCAGTTGAGAGTGATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTAGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGttt                                                                                                                                                                                                             >  1:181287/1‑251 (MQ=255)

                                 ggtggtCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTGAGAGTTATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCtt                                                                                                                                                                                                      <  1:14159/251‑1 (MQ=255)

                                                                  tgtgTTAACATTTCAATACCCTTACAGTTGAGAGTTATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGAt                                                                                                                                                                      <  1:138570/250‑1 (MQ=255)

                                                                   gtgtTAACATTTAAATACACTTAAAGTTGAGAGTACTTGATATGTTGGATGTATTTACTCCATTGTTTAAACTTTTTGTTAATGAGATTCTTGAAAGCCTTTTGTGTAAGTTTATCATTTTTGGTCTCACTCTCTGGTTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGACATACCTAGGCTCTTGCAGATTATAGTTTTTGCATTTTCTTTCGTGGTCTCAATTTCATTTTCAAgtttt                                                                                                                                                                    >  2:242867/1‑247 (MQ=255)

                                                                         aCATTTCAATACCCTTACATTTGAGAGTTATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGca                                                                                                                                                              <  1:252624/251‑1 (MQ=255)

                                                                                                  agTTATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTTCTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATtac                                                                                                                                     >  2:212003/1‑251 (MQ=255)

                                                                                                       ttGATAGGTTGGAAGTATTTACTCCATTGTTTAACCTTTTTGCTAACGCGCCACTCGAAAGACTCCAGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACGGAAAGAGTTTCCTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCTTTTTTTCCTTTTCTTTAGTGGCCGCCATTTCCTTCTCAAGCTTGAGAGACCATCTTCAAAAGCATTATTCATTACTAcc                                                                                                                                 >  2:135215/1‑250 (MQ=255)

                                                                                                                                                       aGCCAATCGACAGACTTATGTATACGATTATAATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGTAttt                                                                                >  2:46037/1‑251 (MQ=255)

                                                                                                                                                            cTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGTATTTaa                                                                              >  2:295095/1‑248 (MQ=255)

                                                                                                                                                                                gATTATCCTTTTTGGTCTCACTCTCTGGCTGCTACCGACAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGTATTTAAAGATTTCCTTAAAACAGGAAATc                                                       >  2:33236/1‑251 (MQ=255)

                                                                                                                                                                                     tCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGTATTTAAAGATTTCCTTAAAACAGGAAATCttatt                                                  >  1:209671/1‑251 (MQ=255)

                                                                                                                                                                                                        ctGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATTCTTATATTGAAATAACTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTCTCGTGGTCGCCATTTCCGTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACTAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGTATTTAAAGATGTCCTTAAAACAGTAAATCTTATTATCACTTCTCCTTGcc                                  <  2:199153/248‑1 (MQ=255)

                                                                                                                                                                                                          ggCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGTATTTAAAGATTTCCTTAAAACAGGAAATCTTATTATCACTTCTCCTTGCCGTAAc                             >  2:2922/1‑251 (MQ=255)

                                                                                                                                                                                                          ggCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACAAATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGTATTTAAAGATTTCCTTAAAACAGGAAATCTTATTATCACTTCTCCTTGCCGTa                               <  2:207906/249‑1 (MQ=255)

                                                                                                                                                                                                                                     cATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGTATTTAAAGATTTCCTTAAAACAGGAAATCTTATTATCACTTCTCCTTGCCGTAACCCGGTTATGAAAAAATTAGAACGGAAg  <  2:27416/251‑1 (MQ=255)

GTGCCCGGTTTTATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTGAGAGTTATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATACTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGTATTTAAAGATTTCCTTAAAACAGGAAATCTTATTATCACTTCTCCTTGCCGTAACCCGGTTATGAAAAAATTAGAACGGAAG  >  NC_000913/1418563‑1419042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`