Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,401,893	G→A	Q455* (CAA→TAA)	abgB ←	p‑aminobenzoyl‑glutamate hydrolase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,401,893	0	G	A	100.0%	71.7 / NA	23	Q455* (CAA→TAA)	abgB	p‑aminobenzoyl‑glutamate hydrolase subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/10);  total (13/10)

CAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACATCAGTCGATGCCGCAAGCACGTTATCGGTGGCGGCATATGG  >  NC_000913/1401666‑1402133                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 cAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTATCATTCTTGCAATTGCCCTGAAt                                                                                                                                                                                                                           <  2:278728/251‑1 (MQ=255)            agagCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAag                                                                                                                                                                                                                   >  2:290310/1‑248 (MQ=255)              agCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGAtt                                                                                                                                                                                                              <  1:228155/251‑1 (MQ=255)                      aGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTgg                                                                                                                                                                                                      >  1:24141/1‑251 (MQ=255)                                       tACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGCGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTc                                                                                                                                                                                     >  1:278611/1‑251 (MQ=255)                                                     aCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCAt                                                                                                                                                                       >  1:149159/1‑251 (MQ=255)                                                        cAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTcc                                                                                                                                                                    <  1:129992/251‑1 (MQ=255)                                                                  gCTTCCCGGATTGGGAGGACGACGGTATGGATGACATAATCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCa                                                                                                                                                          <  2:72902/251‑1 (MQ=255)                                                                                      gacgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCtt                                                                                                                                                                                                                                          >  1:266212/1‑151 (MQ=255)                                                                                      gacgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCtt                                                                                                                                                                                                                                          <  2:266212/151‑1 (MQ=255)                                                                                                                   tCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTa                                                                                                          <  1:270403/250‑1 (MQ=255)                                                                                                                       cGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCg                                                                                                       <  2:24141/249‑1 (MQ=255)                                                                                                                        ggTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACg                                                                                                    >  1:250715/1‑251 (MQ=255)                                                                                                                                               tgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTg                                                                             >  2:266159/1‑251 (MQ=255)                                                                                                                                                  tATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGc                                                                          >  2:294048/1‑251 (MQ=255)                                                                                                                                                        aaaGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAgg                                                                    >  1:3431/1‑251 (MQ=255)                                                                                                                                                                 ggTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTcc                                                            >  1:10612/1‑250 (MQ=255)                                                                                                                                                                                           gTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACATCAGTCGATg                                 <  1:294048/251‑1 (MQ=255)                                                                                                                                                                                             gATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACATCAGTCGATGcc                               >  1:122016/1‑251 (MQ=255)                                                                                                                                                                                                       ggtgtCCGTGACTTGATGATGGTCTTGTTACCATTCTTAAAAGATCCCTGAACCAAGGAAGAGAGTGACTGTGGTTTCGTCAATGGTTTTCTCCGCCAGCATCATTCTGGTATGAGCAATAGATCTTCGCCTCTGTCTAACCAGTTGCCTCGCATGTTGCGGTGTACCGACGGGATAACATGGTCTGAAACAGTGGGTAACAGGCAGTTTCCAACTGACGTCGCCGACTTCAGTCGAGGccactatcgcgt                     <  2:41480/250‑11 (MQ=255)                                                                                                                                                                                                           tCCGTTACTTTCTGATGCTCTTGTTAGTATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGATTGTGGTTGCTGTCATAGTTTTCGCCGCCAGCAGTATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGTCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACGTACGTCGCCGACATCAGTCGATGCTGCATGCACGTTAt                  <  2:194623/250‑1 (MQ=255)                                                                                                                                                                                                                     gCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACATCAGTCGATGCCGCAAGCACGTTATCGGTggcggc        >  1:185177/1‑250 (MQ=255)                                                                                                                                                                                                                            ctctTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGCTTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACATCAGTCGATGCCGCAAGCACGTTATCGGTGGCGGCATATgg  >  1:188109/1‑249 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 CAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACATCAGTCGATGCCGCAAGCACGTTATCGGTGGCGGCATATGG  >  NC_000913/1401666‑1402133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑