Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,387,908	C→T	P397S (CCG→TCG)	tyrR →	DNA‑binding transcriptional dual regulator TyrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,387,908	0	C	T	96.5%	94.3 / NA	29	P397S (CCG→TCG)	tyrR	DNA‑binding transcriptional dual regulator TyrR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (15/13);  minor base G (0/1);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGC  >  NC_000913/1387662‑1388153                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      cTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGa                                                                                                                                                                                                                                                    >  2:143752/1‑250 (MQ=255)          ggCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACAGCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCg                                                                                                                                                                                                                                           <  1:174193/250‑1 (MQ=255)                    gTCGGGTTGGCGAAGACCATGAGGTGACTGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACGGGTGCAAAAAGGCATGTTCCGTGAATATCTCTATTATCGTCTGAACGTGTTAACGCGCAATCGGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAAt                                                                                                                                                                                                                                <  2:85047/251‑1 (MQ=255)                       gggTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACt                                                                                                                                                                                                                             >  2:4309/1‑251 (MQ=255)                             gggAAGACCATGGGGTGCTTGTCGATGTGGGGGTGATTTGCGCTACGAGGAAGAATCTGGTCGATCGGGTCCAAAAAGGAATGTTCCGTGAAGATCTCTATTATCTTCTGAAGGTGTTGACTCTAAATCGGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCTCGCCTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCTGCTGACCGGAATACTGTACtt                                                                                                                                                                                                                       <  2:215730/249‑1 (MQ=255)                               gAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTAc                                                                                                                                                                                                                     <  1:210507/251‑1 (MQ=255)                                        gAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGc                                                                                                                                                                                                            >  2:159326/1‑251 (MQ=255)                                             gCATGTCGATGTGCGGGTTATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGGGCCGCGTTCGAAACTGGCCGCTGACCTGAATTCTGTACTTACGCGTTATGCGTGGc                                                                                                                                                                                                       <  1:265110/251‑1 (MQ=255)                                                     aTGTGCGGGTGATTTGCGCTACGCAGAAGAATATGGTTGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATATGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTAGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAAt                                                                                                                                                                                               >  1:18259/1‑251 (MQ=255)                                                                     cgcTACGCAGAAGAATTTGGTCGAACGGGTGCAAAAAGGAATGTTCCGTGAAGATCTCTTTTATGGTCGGAAGGTGTTGACGCTCAATCTGCCGCCGCTGCGGGACTGTCCGCAGGAAATCATGCCGTTAACTGAGCTGGTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGa                                                                                                                                                                               <  2:39868/251‑1 (MQ=255)                                                                              aaagaaTCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGGGAAGATCTCTATTATCGTCGGAACGGGTTTACGCGCAAGCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACGGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTGCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGctatct                                                                                                                                                                      <  2:38881/250‑1 (MQ=255)                                                                                        gTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACt                                                                                                                                                            >  1:25311/1‑251 (MQ=255)                                                                                             aCTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGacac                                                                                                                                                       <  1:109289/251‑1 (MQ=255)                                                                                                        aaGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACCTCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGt                                                                                                                                            >  2:302379/1‑251 (MQ=255)                                                                                                          ggCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTTCTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTa                                                                                                                                          <  1:307528/251‑1 (MQ=255)                                                                                                                 tCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCt                                                                                                                                    <  1:143752/250‑1 (MQ=255)                                                                                                                           tctcTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAgg                                                                                                                         >  2:101450/1‑251 (MQ=255)                                                                                                                                attatCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATAtt                                                                                                                    >  1:227571/1‑251 (MQ=255)                                                                                                                                     cGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTttgtt                                                                                                               <  2:246143/251‑1 (MQ=255)                                                                                                                                                 ttGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATg                                                                                                    >  1:161443/1‑250 (MQ=255)                                                                                                                                                          aaTCTGCCGCCGCTACGTGACTGTCCGCAGGACACCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAACCTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTAACGCGCACTGACACAACTGGACGGTTATGAGCTGCGTACACAGGATATTTTGTTGCCGGATTATGACGCAGCAAc                                                                                          >  1:284818/1‑251 (MQ=255)                                                                                                                                                                 cgccgcTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGcc                                                                                   <  2:119342/251‑1 (MQ=255)                                                                                                                                                                          gTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGa                                                                           >  1:19590/1‑250 (MQ=255)                                                                                                                                                                                                               gTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGCAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGcc                                     >  2:287816/1‑251 (MQ=255)                                                                                                                                                                                                               gTTCGTCGCCCGATTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGcc                                     >  2:31586/1‑251 (MQ=255)                                                                                                                                                                                                                                 cgacgaGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTAt                   <  2:23496/251‑1 (MQ=255)                                                                                                                                                                                                                                     gaGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAAc               >  2:252764/1‑251 (MQ=255)                                                                                                                                                                                                                                      aGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAAcc              >  1:208022/1‑251 (MQ=255)                                                                                                                                                                                                                                                  cgcgTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCGCCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGc  <  2:25311/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      CTTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGC  >  NC_000913/1387662‑1388153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑