Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,859	T→C	Y110C (TAT→TGT)	puuP ←	putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,859	0	T	C	100.0%	62.8 / NA	21	Y110C (TAT→TGT)	puuP	putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/10);  total (11/10)

CCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAG  >  NC_000913/1358628‑1359094                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            ccTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCa                                                                                                                                                                                                                          <  2:74804/251‑1 (MQ=255)                       gAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTg                                                                                                                                                                                                     <  2:41185/250‑1 (MQ=255)                             ccAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGtt                                                                                                                                                                                               >  1:74147/1‑250 (MQ=255)                              cAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGGGGGGAGAAAGATTTTCGCCAACAAGACGTTAATTATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGAAGGGCGGGTTa                                                                                                                                                                                              >  1:133202/1‑250 (MQ=255)                                                       gagaTTTGAACCAGTACAAACAGGGTATTGAAGTTACCGACGAGGCTAACGATATTCAGATTCGCGGCGGTTAAAAAGGCGACGAAGGTTACCACCCACACCCAAGGCGGCACTTCCAGGACGAGGGGGGAGAGATAGATTTTCGCCAACAAAACGCTAATCATAGGCAAAAAGAAACAATCCAGCAACGAAGACCAGCCGACCCTAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGCATAGGc                                                                                                                                                                    >  1:176703/1‑251 (MQ=255)                                                                             gggTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCCAAAAGAGACAAACCCGCAGCGAAGAACAGCCGACCATAAATCCGACGTGCGGGTTAATCCACTTTTGCGCGTAGGGATAGGCCGAACCGGCCTCCGGGAACTgg                                                                                                                                              >  2:89977/1‑251 (MQ=255)                                                                                             gACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGt                                                                                                                              <  2:242208/251‑1 (MQ=255)                                                                                                 aGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCt                                                                                                                          <  1:65496/251‑1 (MQ=255)                                                                                                    ttGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGAt                                                                                                                       >  1:111040/1‑251 (MQ=255)                                                                                                                       cggcggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACAccc                                                                                                    <  2:53318/251‑1 (MQ=255)                                                                                                                            gTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgccag                                                                                               <  2:111040/251‑1 (MQ=255)                                                                                                                                          gaAGGATACCACCCACACCCACGGCGGCACTTCCGGGAAGCGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCCAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGAATAGGACGAACCGGCCTCCGGAACCTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGACAGCAAATAgg                                                                                 >  2:13256/1‑251 (MQ=255)                                                                                                                                                          acccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGAcc                                                                 >  1:262378/1‑251 (MQ=255)                                                                                                                                                             cacGGCGGCACTTCCGGGAAGAGGGCGGAGAAATAGATTTCCGCCAAAAAGACGTTAATCATCGGCAAAAAGAGACAAGCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCGGAACCGGCCTCCGGAAACTGGCGAACCATTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTc                                                              <  1:145247/251‑1 (MQ=255)                                                                                                                                                                          ccGGGAAGGGGGCGGAGAAATAAATTTTCGCCAACAAGACGTTAACCATCGGAAAAAAGAGACAACCCAGCGGAGATGCCCAGCCGACCATAATCCCGCCGGGCGGTTAAATCGTCTTTGGCGGGAGGGTATAGCCCGACCCGGCCCCCGGAAACTGGCGAACCAGTTTGCGGTAGCGTATAGCGTTAAACACCCCACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGgag                                                 <  2:17563/251‑1 (MQ=255)                                                                                                                                                                                agagGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGAATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAAAAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAAt                                            >  1:38885/1‑250 (MQ=255)                                                                                                                                                                                                      cGCCAACAAGACGTTAATCATCGGCAACAAGATACTCTCCCGCAGCGATGACCAGCCGACCCTAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACCGGCAAACCAGTTTGCCGAAGCTGCTAGCGAAAAACAGCACACCCGCCAGCGCAAGAAAATAGGCCCCCGGACCGGGACCGTCGCTAATGCCGGAAACACTTCCCCAAGCATCTAATACGGTc                      >  2:175606/1‑250 (MQ=255)                                                                                                                                                                                                                  gTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCg                                                                                                                                            >  1:241091/1‑120 (MQ=255)                                                                                                                                                                                                                  gTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCg                                                                                                                                            <  2:241091/120‑1 (MQ=255)                                                                                                                                                                                                                         atcGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGcc    >  1:77838/1‑249 (MQ=255)                                                                                                                                                                                                                         accgccAAAAAGAGACAATCCAGCAGCGATGCCCAGCCGACCATAAATCCGACGGGCGGGTTAATCGACTTTGGCGCGTAGGTATAGGCCGACCCGGCCTCCGGAAACGGGCGACCCAGTTTGCCTTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAg  <  1:138627/246‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            CCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAG  >  NC_000913/1358628‑1359094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑