Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,335,123	C→T	intergenic (+294/‑25)	cysB → / → ymiA	DNA‑binding transcriptional dual regulator CysB/uncharacterized protein YmiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,335,123	0	C	T	100.0%	46.7 / NA	15	intergenic (+294/‑25)	cysB/ymiA	DNA‑binding transcriptional dual regulator CysB/uncharacterized protein YmiA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/6);  total (9/6)

ACTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACG  >  NC_000913/1334893‑1335361                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               aCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCg                                                                                                                                                                                                                             >  1:212756/1‑250 (MQ=255)        tCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATg                                                                                                                                                                                                                     >  1:5200/1‑251 (MQ=255)            taCCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGt                                                                                                                                                                                                                 >  1:20957/1‑251 (MQ=255)                 ggCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGTCTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACTCCAAGCGTTCAACGTTTTGCTGATAAGTGATGGCGATTAGATGAGGTTAGCa                                                                                                                                                                                                            <  2:185369/250‑1 (MQ=255)                                        atTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAAc                                                                                                                                                                                      <  1:182421/250‑1 (MQ=255)                                                   aaaTTACCTGAAAAGTAGTGTCTAATTCCCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATc                                                                                                                                                                          <  1:73275/251‑1 (MQ=255)                                                         ccTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAAt                                                                                                                                                                    >  1:70354/1‑251 (MQ=255)                                                               aaGTAGTGTCTAATTCCCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAATTGAGGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGACCCCCGCCGCGATCCTGAATTAAAAc                                                                                                                                                              <  1:162526/251‑1 (MQ=255)                                                                                               cAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGAt                                                                                                                                                                                                                                        >  1:43748/1‑145 (MQ=255)                                                                                               cAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGAt                                                                                                                                                                                                                                        <  2:43748/145‑1 (MQ=255)                                                                                                           atCTATTTCAGCTCAACAGGTTGAGTTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGACTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGCTTTTCTTGGTTCGGCATTTTTCTgg                                                                                                                  >  2:306337/1‑251 (MQ=255)                                                                                                                    aGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTc                                                                                                         >  1:170494/1‑251 (MQ=255)                                                                                                                                            tGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCCCTTTTCTGGGTGGTTGTCGCACTGCTGATTTTGAAAGTGTgg                                                                                 >  2:230269/1‑251 (MQ=255)                                                                                                                                                  cGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATa                                                                            >  1:26949/1‑250 (MQ=255)                                                                                                                                                                                                                           aTCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGAAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAGaaacgaaacg  <  1:78100/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               ACTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACG  >  NC_000913/1334893‑1335361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑