Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,322,759	G→A	A63V (GCT→GTT)	trpE ←	anthranilate synthase subunit TrpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,322,759	0	G	A	93.8%	45.1 / ‑5.8	16	A63V (GCT→GTT)	trpE	anthranilate synthase subunit TrpE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (8/7);  minor base T (1/0);  total (9/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAGCTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTT  >  NC_000913/1322523‑1322962                                                                                                                                                                                                                                             |                                                                                                                                                                                                            ctcGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATgcgcagc                                                                                                                                                                                               >  2:16698/1‑251 (MQ=255)     ttcttcCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTACAACTGAAATGCGCAGCGCAc                                                                                                                                                                                           >  1:159190/1‑251 (MQ=255)                      tCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACcagcagcag                                                                                                                                                                           <  2:68310/250‑1 (MQ=255)                        aaCAGATTCTGCAATAAACGGAAAGCGTCAAAAATGGAATGGGAGCATAAGCGGGCGTCTTCATGCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTATCTTTGCACACCCGCAGGCAGGGCGTAATCCAGTTGTGCCAGGAAGGCTTCGCCGTTGCCGGTAAGTGCCTGGATTGTGACAGTGTCGACTAAATCTGTAATGAACAGCGCATTGTCTACCAGCAGCATGCt                                                                                                                                                                        >  2:279527/1‑251 (MQ=255)                                 tGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAatc                                                                                                                                                               >  1:168302/1‑251 (MQ=255)                                  gCAATAAACGGAAAGCGTCAAAAACCGAAAGGGGGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCGCACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCATCAGGCTTTTTAAatca                                                                                                                                                              <  2:263373/251‑1 (MQ=255)                                  gCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAat                                                                                                                                                                <  1:16698/249‑1 (MQ=255)                                                            gAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGAACGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTc                                                                                                                                    >  1:152679/1‑251 (MQ=255)                                                               aGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTcc                                                                                                                                   >  2:3501/1‑249 (MQ=255)                                                                             ggCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACCCCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCg                                                                                                                   <  2:253960/251‑1 (MQ=255)                                                                             ggCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCg                                                                                                                   >  1:114796/1‑251 (MQ=255)                                                                                                ggACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTg                                                                                                >  1:154361/1‑251 (MQ=255)                                                                                                         ggggggAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAg                                                                                       <  2:5141/251‑1 (MQ=255)                                                                                                                                 tttGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGGAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATg                                                                                                                                                                                                     >  2:156132/1‑117 (MQ=255)                                                                                                                                 tttGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATg                                                                                                                                                                                                     <  1:156132/117‑1 (MQ=255)                                                                                                                                                                                                tCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAAttt  <  2:114796/249‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                            CTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAGCTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTT  >  NC_000913/1322523‑1322962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑